Nuclear Topology of Murine, Cerebellar Purkinje Neurons: Changes as a Function of Development

Martou, Glyka; Boni, U. De

doi:10.1006/excr.1999.4793

Cited by 68 publications

(69 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Image Analysis-The extent of co-localization of ClC-2 with EEA1 was quantified using a modification of methods described previously (44,45). Briefly, background-corrected EEA1-specific immunofluorescence was subtracted from ClC-2-specific immunofluorescence to yield a "difference" image (using Scion Image; Scion Corporation).…”

Section: Methodsmentioning

confidence: 99%

Evidence for a Functional Interaction between the ClC-2 Chloride Channel and the Retrograde Motor Dynein Complex

Dhani

Mohammad-Panah

Ahmed

et al. 2003

Journal of Biological Chemistry

View full text Add to dashboard Cite

Section: Methodsmentioning

confidence: 99%

Evidence for a Functional Interaction between the ClC-2 Chloride Channel and the Retrograde Motor Dynein Complex

Dhani

Mohammad-Panah

Ahmed

et al. 2003

Journal of Biological Chemistry

View full text Add to dashboard Cite

“…Different postmitotic cells might have distinct nuclear architectures and characteristic higher-order chromosomal organizations (29,30), and there appears to be a close correlation between chromatin structure and gene activation (31)(32)(33). During cellular differentiation, the changes in gene expression patterns may require local or more global alterations of chromatin structures, such as condensation or decondensation of specific chromosomal regions to different degrees of compaction, and thus may require the participation of a type II DNA topoisomerase.…”

Section: Discussionmentioning

confidence: 99%

Aberrant lamination in the cerebral cortex of mouse embryos lacking DNA topoisomerase IIβ

Lyu

Wang²

2003

Proc. Natl. Acad. Sci. U.S.A.

107

115

View full text Add to dashboard Cite

We have examined corticogenesis in mouse embryos lacking DNA topoisomerase II␤ (II␤) in the brain or in all tissues. The absence of II␤, a type II DNA topoisomerase normally expressed in postmitotic cells in the developing cortex, severely affects cerebral stratification: no subplate is discernible, and neurons born at later stages of corticogenesis fail to migrate to the superficial layers. This abnormal pattern of neuron positioning in the cerebral cortex is reminiscent of that observed in mouse mutants defective in the reelin-signaling pathway. Significantly, the level of reelin in the neocortex is much reduced when II␤ is absent. These results implicate a role of II␤ in brain development. The enzyme may be required in implementing particular genetic programs in postmitotic cells, such as reelin expression in Cajal-Retzius cells, perhaps through its action on nucleoprotein structure of particular chromosomal regions.M ammalian DNA topoisomerase II␤ (II␤) is a type II DNA topoisomerase that catalyzes the transport of one DNA double-helix through another (1-3). Unlike the II␣ isozyme, which is specifically expressed in proliferating cells and most likely performs the essential function of resolving intertwined chromosome pairs during mitosis (4-6), II␤ is apparently dispensable in cell growth (reviewed in ref. 7). Targeted disruption of the murine TOP2␤ gene showed; however, that II␤ has a critical role in neural and neuromuscular development (8). Whereas top2␤ ϩ/⌬ mice are phenotypically TOP2␤ ϩ and indistinguishable from their TOP2␤ ϩ/ϩ littermates, top2␤ ⌬/⌬ nullizygotes die of a breathing impairment at birth, most likely owing to neural and neuromuscular defects (8).In mammals, II␣ and II␤ are largely expressed in proliferating cells and nonproliferating cells, respectively (7). In a detailed examination of the expression of the two forms during postnatal development of rat cerebellum, a sharp transition from II␣ to II␤ expression was observed in cell populations that had undergone the final division and committed to differentiate into Purkinje or granule cells (9). The amount of DNA-bound II␤ becomes undetectable, however, when cells reach terminal differentiation (10).To further test the role of II␤ in neural development, we have examined cerebral corticogenesis in embryos of mouse lines lacking II␤ in all tissues or specifically in certain brain structures including the telencephalon. We report here that II␤ has a remarkable role in neurogenesis. The induction of II␤ expression, after the final division of cells committed to a neuronal fate, appears necessary for the developmentally regulated expression of certain specific genes in these cells. The role of II␤ is unlikely to be limited to neuronal development and may also be important in the genetic programming of other postmitotic cells. Materials and MethodsConstruction of the Targeting Vector. The left and right arms of TOP2␤-derived sequences, for targeted insertion of DNA sequences between them into the mouse genome, were obtained by PCR. The right ...

show abstract

“…These data suggest a tissue-specific organization of centromeric heterochromatin. During postnatal development of mice, dynamic clustering processes of centromeres associated with various stages of differentiation were demonstrated in Purkinje cells (21). A centromere repositioning to the nuclear periphery was observed during L6E9 myoblast differentiation (22).…”

mentioning

confidence: 99%

Spatial distribution patterns of interphase centromeres during retinoic acid‐induced differentiation of promyelocytic leukemia cells

et al. 2002

View full text Add to dashboard Cite

Background:The pericentromeric heterochromatin is an important element for the regulation of gene silencing. Its spatial distribution during interphase appears to be celltype specific. This study analyzes three-dimensional (3D) centromere distribution patterns during cellular differentiation along the neutrophil pathway. Methods: Differentiation of the promyelocytic leukemia cell line NB4 was induced by retinoic acid. Centromeres in interphase nuclei were visualized by immunofluorescence staining of centromere-associated proteins with CREST serum. 3D images of nuclei were obtained by confocal microscopy. Automated methods for the segmentation of point-like objects in 3D images were implemented to detect the position of centromeres. Features of centromere localization patterns were determined by constructing the minimal spanning tree of the centromere distribution.

show abstract

Nuclear Topology of Murine, Cerebellar Purkinje Neurons: Changes as a Function of Development

Cited by 68 publications

References 35 publications

Evidence for a Functional Interaction between the ClC-2 Chloride Channel and the Retrograde Motor Dynein Complex

Evidence for a Functional Interaction between the ClC-2 Chloride Channel and the Retrograde Motor Dynein Complex

Aberrant lamination in the cerebral cortex of mouse embryos lacking DNA topoisomerase IIβ

Spatial distribution patterns of interphase centromeres during retinoic acid‐induced differentiation of promyelocytic leukemia cells

Contact Info

Product

Resources

About