Nuclear Transfer: Preservation of a Nuclear Genome at the Expense of Its Associated mtDNA Genome(s)

Bowles, Emma J.; Campbell, Keith; John, Justin C. St.

doi:10.1016/s0070-2153(06)77010-7

Cited by 49 publications

(31 citation statements)

References 226 publications

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“…Influences on the propagation of mitochondria and mtDNA could be of great importance in determining the early developmental potential of SCNT embryos [5,34]. Accordingly, many abnormalities observed in SCNT fetuses and offspring may be influenced by deficiencies in mitochondrial function.…”

Section: Discussionmentioning

confidence: 99%

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Comparative Proteomic Analysis of Liver Mitochondrial Proteins Derived from Cloned Adult Pigs Reconstructed with Meishan Pig Fibroblast Cells and European Pig Enucleated Oocytes

Takeda

Tasai

Iwamoto³

et al. 2012

Journal of Reproduction and Development

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Abstract. Somatic cell nuclear transfer (SCNT) has been exploited in efforts to clone and propagate valuable animal lineages. However, in many instances, recipient oocytes are obtained from sources independent of donor cell populations. As such, influences of potential nuclear-cytoplasmic incompatibility, post SCNT, are largely unknown. In the present study, alterations in mitochondrial protein levels were investigated in adult SCNT pigs produced by microinjection of Meishan pig fetus fibroblast cells into enucleated matured oocytes (maternal Landrace genetic background). Mitochondrial fractions were prepared from liver samples by mechanical homogenization and differential centrifugation. Liver mitochondria were then subjected to two-dimensional difference gel electrophoresis (2-D DIGE). Protein expression changes were confirmed with a volume ratio greater than 2 fold (P < 0.05). 2-D DIGE analysis further revealed differential expression of three proteins between the Meishan (n=3) and Landrace (n=3) breeds. Differential expression patterns of 16 proteins were detected in SCNT pig liver tissue (n=3) when compared with Meishan control samples. However, none of the 16 proteins correlated with the three differentially expressed Meishan and Landrace liver mitochondrial proteins. In summary, alteration of mitochondrial protein expression levels was observed in adult SCNT pigs that did not reflect the breed difference of the recipient oocytes. Comparative proteomic analysis represents an important tool for further studies on SCNT animals.

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Section: Discussionmentioning

confidence: 99%

“…Aberrant reprogramming of donor somatic cell nuclei may result in many severe problems in animal cloning [2,3]. From a mitochondrial biology perspective, the inability to establish functional interactions between the donor nucleus and recipient mitochondria is also likely responsible for such a developmental deficiency [4][5][6].…”

mentioning

confidence: 99%

Comparative Proteomic Analysis of Liver Mitochondrial Proteins Derived from Cloned Adult Pigs Reconstructed with Meishan Pig Fibroblast Cells and European Pig Enucleated Oocytes

Takeda

Tasai

Iwamoto³

et al. 2012

Journal of Reproduction and Development

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“…Não tem sido demonstrado ainda que os clones que apresentam DNA mitocondrial, do oócito e da célula somática, tenham maior probabilidade de apresentar alterações fenotípicas ou até perda embrionária. Mas algumas doenças metabólicas em animais clonados adultos poderiam ser decorrentes da presença de DNA mitocondrial da célula somática (Spikings et al, 2007Bowles et al, 2007).…”

Section: O Genoma Mitocondrialunclassified

Clonagem de embriões bovinos a partir de células somáticas

2014

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Titulo en español: Clonación de embriones bovinos a partir de células somáticastitulo en ingles: Cloning bovine embryos from somatic cellsResumo: No presente artigo revisa-se dedicadamente a literatura do tema da clonagem tentando levar o leitor paulatinamente através dos avanços da técnica até alcançar as mais novas alternativas propostas pelos cientistas, e incluindo os erros mais comumente acontecidos nelas. A finalidade é mostrar o amplio panorama que tem aberto esta biotecnologia para a humanidade toda.Palavras chave: Transferência nuclear, fissão, paraclonagem, clonagem verdadeira, genoma mitocondrial.Resumen: En el presente artículo se revisa detenidamente la literatura en el tema de clonación, intentando llevar al lector paulatinamente a través de los avances de la técnica hasta alcanzar las más nuevas alternativas propuestas por los investigadores, incluyendo los errores más comúnmente cometidos. La finalidad es mostrar el amplio panorama que a abierto esta biotecnología para la humanidad.Palabras clave: Transferência nuclear, fissão, paraclonagem, clonagem verdadeira, genoma mitocondrial.Abstract: The present article provides a review of the pertinent literature on the topic of cloning. It aims to take the reader gradually through the advances made so far regarding the technique, to the newest alternatives proposed by researchers, including the most commonly committed errors, to unveil the broad panorama this biotechnology has opened up for humankind.Key words: Nuclear transfer, fission, paraclone colony, true cloning, mitochondrial genome.

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“…The use of ES cell-like cells (induced pluripotent stem iPS cells) instead of human ES cells might provide wide applications in the field of regenerative medicine. Additionally, donor oocyte mitochondria may present another technical hurdle since they will put non-self antigens in the nuclear transfer cells (Bowles et al, 2007;Harvey et al, 2007).…”

Section: Human Es Cellsmentioning

confidence: 99%

Stem cell plasticity: Learning from hepatogenic differentiation strategies

Banas¹,

Yamamoto

Teratani³

et al. 2007

Developmental Dynamics

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Many studies on stem cell plasticity are challenging the concept that stem cells contain an intrinsically predefined, unidirectional differentiation program. This means that the developmental fate of a stem cell is dependent on the general potential of the cell (pre-determined stem cell fate) as well as on microenvironmental cues, such as stimuli from growth factors (stem cell niche). Here, we reviewed reports that examined the hepatocyte differentiation ability of stem cells from two different sources: embryonic stem cells and adult stem cells. All of those stem cells revealed the ability to give rise to hepatocyte-like cells using different induction strategies. However, it is still not clear which of those stem cells would be the best source for hepatocyte replacement or which would be the best protocol. We herein present the current knowledge regarding available protocols and factors used in order to obtain functional hepatocytes from stem cells. Developmental Dynamics 236:3228 -3241, 2007.

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Nuclear Transfer: Preservation of a Nuclear Genome at the Expense of Its Associated mtDNA Genome(s)

Cited by 49 publications

References 226 publications

Comparative Proteomic Analysis of Liver Mitochondrial Proteins Derived from Cloned Adult Pigs Reconstructed with Meishan Pig Fibroblast Cells and European Pig Enucleated Oocytes

Comparative Proteomic Analysis of Liver Mitochondrial Proteins Derived from Cloned Adult Pigs Reconstructed with Meishan Pig Fibroblast Cells and European Pig Enucleated Oocytes

Clonagem de embriões bovinos a partir de células somáticas

Stem cell plasticity: Learning from hepatogenic differentiation strategies

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