Nuclear translocation of the p65 subunit of NF-κB in L5178Y sublines differing in antioxidant defense

Sochanowicz, Barbara; Szumiel, Irena; Grądzka, I

doi:10.1007/s004110050148

Cited by 4 publications

(2 citation statements)

References 29 publications

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“…During stress conditions, NF-κB transcription factor subunit (p65) translocates into the nucleus, where it binds to several regulatory proteins and regulates gene transcription. So, nuclear import is a key event of NF-κB activation ( 26 ) . Thus, deregulation of NF-κB and IκBα phosphorylation is crucial in inflammation and cancer ( 3 ) , and it has become a popular target for therapeutic applications.…”

Section: Resultsmentioning

confidence: 99%

Antioxidative effects of the spice cardamom against non-melanoma skin cancer by modulating nuclear factor erythroid-2-related factor 2 and NF-κB signalling pathways

Das

Acharya²,

Berry

et al. 2011

Br J Nutr

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The role of dietary factors in inhibiting or delaying the development of non-melanoma skin cancer (NMSC) has been investigated for many years. Cardamom, which is a dietary phytoproduct, has been commonly used in cuisines for flavour and has numerous health benefits, such as improving digestion and stimulating metabolism and having antitumorigenic effects. We have investigated the efficacy of dietary cardamom against 7,12-dimethylbenz[a]anthracene (DMBA)-induced skin papillomatogenesis in Swiss albino mice that closely resembles human NMSC. Mice were grouped into normal wild type (untreated), vehicle-treated (acetone), carcinogen-treated (DMBA), and DMBA and cardamom-treated (DMBA þ CARD) to delineate the role of cardamom against DMBA-induced papillomatogenesis. Oral administration of cardamom to DMBA-treated mice up-regulated the phase II detoxification enzymes, such as glutathione-S-transferase and glutathione peroxidase, probably via activation of nuclear factor erythroid-2-related factor 2 transcription factor in 'DMBA þ CARD' mice. Furthermore, reduced glutathione, glutathione reductase, superoxide dismutase and catalase were also up-regulated by cardamom in the same 'DMBA þ CARD' group of mice compared with DMBA-treated mice. Cardamom ingestion in DMBA-treated mice blocked NF-kB activation and down-regulated cyclo-oxygenase-2 expression. As a consequence, both the size and the number of skin papillomas generated on the skin due to the DMBA treatment were reduced in the 'DMBA þ CARD' group. Thus, the results from the present study suggest that cardamom has a potential to become a pivotal chemopreventive agent to prevent papillomagenesis on the skin.

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Section: Resultsmentioning

confidence: 99%

Antioxidative effects of the spice cardamom against non-melanoma skin cancer by modulating nuclear factor erythroid-2-related factor 2 and NF-κB signalling pathways

Das

Acharya²,

Berry

et al. 2011

Br J Nutr

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“…As a consequence -there is a substantial difference in the initial DNA damage and the lethal effect of hydrogen peroxide treatment (at micromolar concentrations). The status of the antioxidant defence: thiol content and the activity of catalase and 886 E. Bou¿yk and others 2000 Sochanowicz et al, 1999 glutathione peroxidase contribute to the difference, whereas the activation of NF-kB in the pair of LY sublines corresponds with the antioxidant status.…”

Section: Discussionmentioning

confidence: 98%

The response of L5178Y lymphoma sublines to oxidative stress: antioxidant defence, iron content and nuclear translocation of the p65 subunit of NF-kappaB.

Boużyk

Grądzka²,

Iwaneńko³

et al. 2000

Acta Biochim Pol

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We examined the response to hydrogen peroxide of two L5178Y (LY) sublines which are inversely cross-sensitive to hydrogen peroxide and X-rays: LY-R cells are radioresistant and hydrogen peroxide-sensitive, whereas LY-S cells are radiosensitive and hydrogen peroxide-resistant. Higher initial DNA breaks and higher iron content (potentially active in the Fenton reaction) were found in the hydrogen peroxide sensitive LY-R cells than in the hydrogen peroxide resistant LY-S cells, whereas the antioxidant defence of LY-R cells was weaker. In particular, catalase activity is twofold higher in LY-S than in LY-R cells. The content of monobromobimane-reactive thiols is 54% higher in LY-S than in LY-R cells. In contrast, the activity of glutathione peroxidase (GPx) is about two times higher in LY-R than in LY-S cells; however, upon induction with selenium the activity increases 15.6-fold in LY-R cells and 50.3-fold in LY-S cells.Altogether, the sensitivity difference is related to the iron content, the amount of the initial DNA damage, as well as to the efficiency of the antioxidant defence system. oxygen species, SOD, superoxide dismutase. ferential nuclear translocation of p65-NF-kB in LY sublines is due to the more efficient antioxidant defence in LY-S than in LY-R cells.Short arrows indicate the modified stages of activation (see text for explanations).

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Intraclonal recovery of ‘slow clones’—a manifestation of genomic instability: Are mitochondria the key to an explanation?

Szumiel

2014

Radiat Environ Biophys

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Intraclonal recovery following X-irradiation in an in vitro study of L5178Y-S murine leukaemic cells is reviewed. This phenomenon was first described in 1994 occurring in the slowly growing clones ('slow clones') present among the survivors in irradiated cell populations. An attempt to explain these experimental data is given in terms of the present knowledge of the role of mitochondria in nontargeted radiation effects, with the focus on genomic instability and mtDNA-related epigenetic modifications of the nuclear genome. An understanding of this intraclonal recovery may be important in preventing tumour regrowth following radiotherapy, as well as in decreasing the risk of secondary radiation-induced malignancies.

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Nuclear translocation of the p65 subunit of NF-κB in L5178Y sublines differing in antioxidant defense

Cited by 4 publications

References 29 publications

Antioxidative effects of the spice cardamom against non-melanoma skin cancer by modulating nuclear factor erythroid-2-related factor 2 and NF-κB signalling pathways

Antioxidative effects of the spice cardamom against non-melanoma skin cancer by modulating nuclear factor erythroid-2-related factor 2 and NF-κB signalling pathways

The response of L5178Y lymphoma sublines to oxidative stress: antioxidant defence, iron content and nuclear translocation of the p65 subunit of NF-kappaB.

Intraclonal recovery of ‘slow clones’—a manifestation of genomic instability: Are mitochondria the key to an explanation?

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