Nuclear transporters in a multinucleated organism: functional and localization analyses in <i>Aspergillus nidulans</i>

Markina-Iñarrairaegui, Ane; Etxebeste, Oier; Herrero-García, Erika; Araújo‐Bazán, Lidia; Fernández-Martı́nez, Javier; Flores, Jairo A.; Osmani, Stephen A.; Espeso, Eduardo A.

doi:10.1091/mbc.e11-03-0262

Cited by 36 publications

(58 citation statements)

References 75 publications

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“…In this way, the cell ensures that CreA is always correctly localized. Similar observations have been made for the alkaline pH response transcription factor and for CrzA, a transcription factor involved in modulating the cellular response to calcium levels and alkaline pH stress (Fernández-Martínez et al 2003;Markina-Iñarrairaegui et al 2011;Hernández-Ortiz and Espeso 2013). No nuclear localization signal (NLS) was predicted to be contained within CreA in this work or in previous studies (Roy et al 2008).…”

Section: Discussionsupporting

confidence: 85%

Diverse Regulation of the CreA Carbon Catabolite Repressor in Aspergillus nidulans

et al. 2016

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Carbon catabolite repression (CCR) is a process that selects the energetically most favorable carbon source in an environment. CCR represses the use of less favorable carbon sources when a better source is available. Glucose is the preferential carbon source for most microorganisms because it is rapidly metabolized, generating quick energy for growth. In the filamentous fungus Aspergillus nidulans, CCR is mediated by the transcription factor CreA, a C 2 H 2 finger domain DNA-binding protein. The aim of this work was to investigate the regulation of CreA and characterize its functionally distinct protein domains. CreA depends in part on de novo protein synthesis and is regulated in part by ubiquitination. CreC, the scaffold protein in the CreB-CreC deubiquitination (DUB) complex, is essential for CreA function and stability. Deletion of select protein domains in CreA resulted in persistent nuclear localization and target gene repression. A region in CreA conserved between Aspergillus spp. and Trichoderma reesei was identified as essential for growth on various carbon, nitrogen, and lipid sources. In addition, a role of CreA in amino acid transport and nitrogen assimilation was observed. Taken together, these results indicate previously unidentified functions of this important transcription factor. These novel functions serve as a basis for additional research in fungal carbon metabolism with the potential aim to improve fungal industrial applications.

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Section: Discussionsupporting

confidence: 85%

Diverse Regulation of the CreA Carbon Catabolite Repressor in Aspergillus nidulans

et al. 2016

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“…One possibility is that AreA may use alternative importins for nuclear import under different growth conditions due to differential expression of importins. A. nidulans has 17 nuclear importins, but the expression of these across different growth conditions has not been determined (12). Alternatively, multiple NLSs could allow for more-efficient nuclear import.…”

Section: Discussionmentioning

confidence: 99%

Multiple Nuclear Localization Signals Mediate Nuclear Localization of the GATA Transcription Factor AreA

et al. 2014

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“…Strain MAD1425 (pyrG89,argB2;pyroA4,nkuA∆::argB) [28] was used as a recipient for the generation of strains expressing GFP tagged versions of GskA, driven by the gskA endogenous promoter, strain MAD5515 (pyrG89,argB2;pyroA4,nkuA∆::argB;gskA-gfp-pyrG Af ), and by the alcA M A N U S C R I P T…”

Section: Strains and Mediamentioning

confidence: 99%

“…Strains CM237, AF293, CBS144 are wildtype prototrophs of A. fumigatus. A. nidulans strains were cultivated in appropriately supplemented standard minimal medium (AMM) and complete medium (ACM) for propagation and obtaining conidiospores, as described previously [28]. For total protein extractions, strains were cultivated in fermentation media [12] supplemented with 1% Glucose or 1% Ethanol for 18h…”

Section: A C C E P T E D Accepted Manuscriptmentioning

confidence: 99%

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New applications for known drugs: Human glycogen synthase kinase 3 inhibitors as modulators of Aspergillus fumigatus growth

Sebastián-Pérez

Manoli

Pérez

et al. 2016

European Journal of Medicinal Chemistry

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Invasive aspergillosis (IA) is one of the most severe forms of fungi infection. IA disease is mainly due to Aspergillus fumigatus, an air-borne opportunistic pathogen. Mortality rate caused by IA is still very high (50-95%), because of difficulty in early diagnostics and reduced antifungal treatment options, thus new and efficient drugs are necessary. The aim of this work is, using Aspergillus nidulans as non-pathogen model, to develop efficient drugs to treat IA. The recent discovered role of glycogen synthase kinase-3 homologue, GskA, in A. fumigatus human infection and our previous experience on human GSK-3 inhibitors focus our attention on this kinase as a target for the development of antifungal drugs. With the aim to identify effective inhibitors of colonial growth of A. fumigatus we use A. nidulans as an accurate model for in vivo and in silico studies. Several well-known human GSK-3β inhibitors were tested for inhibition of A. nidulans colony growth. Computational tools as docking studies and binding site prediction was used to explain the different biological profile of the tested inhibitors. Three of the five tested hGSK3β inhibitors are able to reduce completely the colonial growth by covalent bind to the enzyme. Therefore these compounds may be useful in different applications to eradicate IA.

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Nuclear transporters in a multinucleated organism: functional and localization analyses in Aspergillus nidulans

Cited by 36 publications

References 75 publications

Diverse Regulation of the CreA Carbon Catabolite Repressor in Aspergillus nidulans

Diverse Regulation of the CreA Carbon Catabolite Repressor in Aspergillus nidulans

Multiple Nuclear Localization Signals Mediate Nuclear Localization of the GATA Transcription Factor AreA

New applications for known drugs: Human glycogen synthase kinase 3 inhibitors as modulators of Aspergillus fumigatus growth

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