Nuclease Activity via Self-Activation and Anticancer Activity of a Mononuclear Copper(II) Complex: Novel Role of the Tertiary Butyl Group in the Ligand Frame

Abstract

Copper complex [Cu(tBuPhimp)(Cl)] (1) derived from tridentate ligand tBuPhimpH having N2O donors was synthesized and molecular structure was determined. Phenoxyl radical complex was generated in solution at room temperature using Ce(IV) salt. Nuclease activity and anticancer activity of 1 was investigated. Roles of tert-butyl group and singlet oxygen in DNA cleavage activity were also discussed.

Oxidation chemistry of C–H bond by mononuclear iron complexes derived from tridentate ligands containing phenolato function

“…This also supports that it is a metal based oxidation reaction [61][62][63][64][65][66]. It is interesting to note that the copper complex described in our previous report [33] the substrate and radical species convert the reactant into the corresponding product [79]. The possible mechanism for oxidation reaction using the complex 4 was shown above the removal of the chloride ion followed by the addition of peroxide molecule generate the active Fig.…”

“…synthesized by reacting 2-(1-phenylhydrazinyl)-pyridine with 2-hydroxy-5-methylbenzaldehyde, 2-hydroxy-5-nitrobenzaldehyde, 2-hydroxy-5-methoxybenzaldehyde and 2-hydroxy-5-methoxybenzaldehyde and 3,5-di-tert-butyl-2-hydroxybenzaldehyde respectively in methanol following reported procedure [33][34][35]. Details of their synthesis are described in the Supporting Information (Scheme S1) Complex 3 was synthesized following the same procedure described above using ligand (L 3 H) (159.00 mg, 0.5 mmol), NaH (12.00 mg, 0.5mmol) and FeCl 3 (82.00 mg , 0.5 mmol).…”

“…All the ligands were prepared following the same procedure [33]. The metal complexes (1−4) were synthesized according to the procedures reported by Ghosh and co-workers [35].…”

Spontaneous Reduction of Mononuclear High‐Spin Iron(III) Complexes to Mononuclear Low‐Spin Iron(II) Complexes in Aqueous Media and Nuclease Activity via Self‐Activation

“…In fact, during nuclease activity studies an oxidizing agent like hydrogen peroxide and/or reducing agent such as 2‐mercapto ethanol or ascorbic acid were utilized. We have investigated the nuclease activity during spontaneous reduction because the reduction process occurred in the absence of any reducing agent which probably could lead to DNA cleavage via self‐activation 10b,d. To the best of our knowledge, there is no report available in the literature where reactive species produced during spontaneous reduction process were utilized for nuclease activity studies.…”

Structural characterization, ROS-inductive and proteasome inhibitory properties of ternary and binary copper(II) complexes of N2- and N2O2-ligands

“…ascorbic acid) or oxidant (e.g. hydrogen peroxide H 2 O 2 ) but the ROS-generating ability of these copper(II) complexes are rarely quantitatively compared by using ROS-detection assay [19][20][21][22]. Similarly, there is seldom quantitative comparison of copper(II) complexes with the ROS-generating ability of their precursor copper(II) salts and free ligands.…”