Nuclease expression in efficient polyhydroxyalkanoates-producing bacteria could yield cost reduction during downstream processing

Gamero, Jesus Enrique Rodriguez; Favaro, Lorenzo; Pizzocchero, Valentino; Lomolino, Giovanna; Basaglia, Marina; Casella, Sergio

doi:10.1016/j.biortech.2018.04.021

Cited by 27 publications

(11 citation statements)

References 40 publications

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“…183 This approach became object of a patent 184 and later the same nuclease gene has been integrated and expressed in two efficient PHA-producing bacteria: C. necator and D. acidovorans. 185 While PHA production was not affected in both hosts, the viscosity of the lysates deriving from recombinant C. necator cells was greatly reduced, indicating that the engineered strain is expected to yield large reduction costs in PHAs downstream processing. Moreover, a system for increasing oxygen availability was successfully developed by cloning the VHb gene (encoding Vitreoscilla hemoglobin) in Halomonas under the strong low-oxygen inducible promoter P 8vgb , which also controls phaCAB operon.…”

Section: Further Approaches To Reduce Pha Production Costsmentioning

confidence: 94%

“…Integrating a nuclease‐encoding gene of Staphylococcus aureus into the genomes of several PHA producers was successfully achieved . This approach became object of a patent and later the same nuclease gene has been integrated and expressed in two efficient PHA‐producing bacteria: C. necator and D. acidovorans . While PHA production was not affected in both hosts, the viscosity of the lysates deriving from recombinant C. necator cells was greatly reduced, indicating that the engineered strain is expected to yield large reduction costs in PHAs downstream processing.…”

Section: Further Approaches To Reduce Pha Production Costsmentioning

confidence: 99%

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Improving polyhydroxyalkanoate production from inexpensive carbon sources by genetic approaches: a review

Favaro

Basaglia

Casella

2018

Biofuels Bioprod Bioref

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137

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Polyhydroxyalkanoates (PHAs) are a family of biodegradable intracellular polyesters that a number of Eubacteria and Archaea can accumulate for energy and carbon storage. Most of the genetic modifications to the producing bacterial species have been accomplished to clarify basic biochemical, genetic, and metabolic aspects of PHA metabolism. However, due to its plastic‐like properties and complete biodegradability, this bio‐based polymer has attracted the attention of a variety of manufacturers. A number of genetic approaches have therefore been reported, aimed at improving the performance of the microorganisms with a potential for use in a production process. Indeed, genetic tools may find useful applications in all the phases of the PHA production chain, from the isolation and characterization of new microbial strains through all the production steps until they reach the downstream processes. The substrates generally used for PHA production are expensive, so the search for low‐cost feedstock is necessary. These materials, possibly deriving from agri‐food processes, are unfortunately not easily degraded or converted directly into PHAs. Thus, the development of engineered microbes is in progress to process waste streams and covert them to valuable polymers. This review will summarize the most relevant results obtained through genetic engineering tools for the production of PHAs from cheap carbon sources in view of possible industrial applications. © 2018 Society of Chemical Industry and John Wiley & Sons, Ltd.

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Section: Further Approaches To Reduce Pha Production Costsmentioning

confidence: 94%

Section: Further Approaches To Reduce Pha Production Costsmentioning

confidence: 99%

Improving polyhydroxyalkanoate production from inexpensive carbon sources by genetic approaches: a review

Favaro

Basaglia

Casella

2018

Biofuels Bioprod Bioref

Self Cite

137

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“…The gas carrier was helium at a flow rate 1.2 mL/min and the split/splitless injector with a split ratio of 1:30 was set at 250 • C. The FID and oven temperature was set at 270 and 150 • C, respectively. Benzoic acid was used as an internal standard whereas the external standards, 3-hydroxybutyric acid (3HB), poly(3-hydroxybutyric acid-co-3-hydroxyvaleric acid P(3HB-co-12 mol% 3HV) and poly(3-hydroxybutyric acid-co-4-hydroxybytyric acid) P(3HB-co-11.2 mol% 4HB), were purchased from Sigma-Aldrich (Italy) [50].…”

Section: Phas Analysismentioning

confidence: 99%

Conversion of Starchy Waste Streams into Polyhydroxyalkanoates Using Cupriavidus necator DSM 545

et al. 2020

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Due to oil shortage and environmental problems, synthetic plastics have to be replaced by different biodegradable materials. A promising alternative could be polyhydroxyalkanoates (PHAs), and the low-cost abundant agricultural starchy by-products could be usefully converted into PHAs by properly selected and/or developed microbes. Among the widely available starchy waste streams, a variety of residues have been explored as substrates, such as broken, discolored, unripe rice and white or purple sweet potato waste. Cupriavidus necator DSM 545, a well-known producer of PHAs, was adopted in a simultaneous saccharification and fermentation (SSF) process through an optimized dosage of the commercial amylases cocktail STARGEN™ 002. Broken rice was found to be the most promising carbon source with PHAs levels of up to 5.18 g/L. This research demonstrates that rice and sweet potato waste are low-cost feedstocks for PHAs production, paving the way for the processing of other starchy materials into bioplastics.

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“…To cut down costs, nucleases encoding genes from Staphylococcus aureus were inserted in PHA producing strains such as P. putida and C. necator. In both cases, the lysate viscosity was successfully was reduced without compromising the PHA production [103,104].…”

Section: High Pressure Homogenizationmentioning

confidence: 97%

A sustainable approach for the downstream processing of bacterial polyhydroxyalkanoates: State-of-the-art and latest developments

Pérez-Rivero

López‐Gómez

Roy

2019

Biochemical Engineering Journal

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Bioplastics have emerged as a platform to reduce our dependence on fossil fuels. Polyhydroxyalkanoates (PHAs) are a family of biodegradable polyesters with large potential in consumer goods and medical applications. These polymers accumulate in prokaryotic microbes and their recovery is a challenging, often under explored, operation. In the past, oil-derived solvents and chemicals have been widely used as extracting agents, compromising the "environmentally-friendly" claim of bioplastics. Furthermore, the large amount of chemicals and solvents required at the industrial level would negatively impact the economics of the process. The present review presents the latest advances in the field of downstream operations for PHA recovery emphasizing those green technologies with scaling-up feasibility. As for the upstream and fermentation stages, the extraction process needs to be carefully optimized to accomplish a competitive production of PHAs.

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Nuclease expression in efficient polyhydroxyalkanoates-producing bacteria could yield cost reduction during downstream processing

Cited by 27 publications

References 40 publications

Improving polyhydroxyalkanoate production from inexpensive carbon sources by genetic approaches: a review

Improving polyhydroxyalkanoate production from inexpensive carbon sources by genetic approaches: a review

Conversion of Starchy Waste Streams into Polyhydroxyalkanoates Using Cupriavidus necator DSM 545

A sustainable approach for the downstream processing of bacterial polyhydroxyalkanoates: State-of-the-art and latest developments

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