Nuclease-resistant DNA aptamer on gold nanoparticles for the simultaneous detection of Pb2+ and Hg2+ in human serum

Chung, Chan Ho; Kim, Joong Hyun; Jung, Juyeon; Chung, Bong Hyun

doi:10.1016/j.bios.2012.10.026

Cited by 128 publications

(42 citation statements)

References 39 publications

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“…A number of methods have been reported to synthesize Au NCs by using specific capping agents such as thiol ligands [42], or using templates such as DNA [7,43] and polymers [44]. These Au NCs have been used in the determination of various heavy metals such as Hg 2+ [8,34,35,45,46], Ag + [14,15,38], Cu 2+ [30], Fe 3+ [47], As 3+ [48], and Pb 2+ [49] with advances of being simple, rapid, and economical. Thus it is possible to utilize Au NCs as a fluorescent probe for selective determination of Ag + and Hg 2+ , which is valuable for monitoring the relevant environment and human health [10,50].…”

Section: Introductionmentioning

confidence: 99%

Cytidine-stabilized gold nanocluster as a fluorescence turn-on and turn-off probe for dual functional detection of Ag+ and Hg2+

Zhang

Jiang

Wang

2015

Analytica Chimica Acta

129

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Section: Introductionmentioning

confidence: 99%

Cytidine-stabilized gold nanocluster as a fluorescence turn-on and turn-off probe for dual functional detection of Ag+ and Hg2+

Zhang

Jiang

Wang

2015

Analytica Chimica Acta

129

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“…Summing up all aforesaid, the majority of the described test systems requires an additional step of the conjugation between colloidal gold and an affine-recognizing molecule on the surface (Chung et al 2013;Liu, Lu 2003) to provide high specificity and sensitivity to lead. A smaller number of the developed test systems are based on the use of non-conjugated colloidal gold particles.…”

Section: Introductionmentioning

confidence: 99%

“…Various nanoparticle modification processes have been used to develop methods for the determination of lead, and include DNA, oligonucleotides Chung et al 2013), DNAzymes (Kim, Han, Chung 2011;Liu, Lu 2003;Miao, Ling, Shuai 2012), and peptides (Zhu et al 2012) as modifiers. Mercapto compounds have also been widely used for the development of analytical methods for mercury and lead.…”

Section: Introductionmentioning

confidence: 99%

Colorimetric Determination of Lead Using Gold Nanoparticles

et al. 2014

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A combined homogeneous assay and colorimetric determination method using gold nanoparticles was developed for rapid determination of lead(II) in contaminated natural waters. The presence of lead(II) in the colloidal gold suspension causes a change in the absorbance of the suspension. An increase in the absorption property at 595 nm is accompanied by a change in the size of the gold nanoparticles. High concentrations of lead cause aggregation of the gold colloids. Colloidal gold nanoparticles were synthesized using tannic acid as the reducing agent; this reagent allowed selective determination of lead in 10 µL of water, with a detection limit of 310 ng mL −1 within an analysis time of 5 min. The coefficient of variation for lead(II) within the working range of the assay (520 ng mL −1 13 µg mL −1 ) varied from 1.3 to 9.2%. The limit of detection using this method with a sample volume of 50 µL was 60 ng mL −1 . The coefficient of variation for lead over the working range of the determined concentrations (80 ng mL −1 25 µg mL −1 ) varied from 0.2 to 9.3%, while the values for the interday assay (n=8) were less than 10%. The method was employed for the analysis of river, lake, marsh, and Downloaded by [University of Washington Libraries] at 02:13 29 November 2014 2 spring water; the recovery of lead was determined to be 72.5-130% for 10 µL of water and 93.6 -114.7% for 50 µL.

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“…32 However, one of the most important properties of DNA-coated AuNPs is there great intracellular stability and increased resistance towards degradation by nucleases. 30,31,33 Research that involves the use of nucleic acids in biological environments is often hindered by the process of enzymatic hydrolysis, which leads to their degradation and renders them inactive. 34 DNA-coated AuNPs have shown an increased resistance to nucleases such as DNaseI.…”

Section: Introductionmentioning

confidence: 99%

Investigating the stability of DNA-coated gold nanoparticles

Kyriazi

Kanaras

2018

Colloidal Nanoparticles for Biomedical Applications XIII

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In this paper we investigate how the density of oligonucleotides around spherical 15 nm gold nanoparticles (AuNPs) influences their stability against degradation by DNase I. We show that a significant decrease in the number of oligonucleotides attached to the AuNP surface is directly correlated with an increase in the DNA degradation by DNase I. Our experimental observations suggest that a close packing of oligonucleotides into a 3d arrangement at the surface of AuNPs endows the probes with the necessary stability required for their use in intracellular applications.

show abstract

Nuclease-resistant DNA aptamer on gold nanoparticles for the simultaneous detection of Pb2+ and Hg2+ in human serum

Cited by 128 publications

References 39 publications

Cytidine-stabilized gold nanocluster as a fluorescence turn-on and turn-off probe for dual functional detection of Ag+ and Hg2+

Cytidine-stabilized gold nanocluster as a fluorescence turn-on and turn-off probe for dual functional detection of Ag+ and Hg2+

Colorimetric Determination of Lead Using Gold Nanoparticles

Investigating the stability of DNA-coated gold nanoparticles

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