Nucleated Red Blood Cells Contribute to the Host Immune Response Against Pathogens

Chico, V.; Nombela, Iván; Puente-Marín, Sara; Ortega-Villaizán, M.

doi:10.5772/intechopen.80545

Cited by 9 publications

(16 citation statements)

References 74 publications

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“…Notably, we observed a small part of nucleated erythrocytes unexpectedly expressing signatures related to innate immune (neutrophil, phagocytosis and inflammatory response) and adaptive immune (antigen processing and presentation) reactions. It has been reported that nucleated erythrocytes of human cord blood suppressed the production of inflammatory cytokines from monocytes in a lipopolysaccharide (LPS)-mimicked system, to avoid a vigorous innate immune reaction [ 24 ]. Our results from adult bone marrow further confirmed the mediator roles of nucleated erythrocytes in immune responses.…”

Section: Discussionmentioning

confidence: 99%

Single-cell transcriptomic landscape of human blood cells

Xie

Liu

Zhang

et al. 2020

National Science Review

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High throughput single-cell RNA-seq has been successfully implemented to dissect the cellular and molecular features underlying hematopoiesis. However, an elaborate and comprehensive transcriptome reference of the whole blood system is lacking. Here, we profiled the transcriptomes of 7,551 human blood cells representing 32 immunophenotypic cell types, including hematopoietic stem cells, progenitors and mature blood cells derived from 21 healthy donors. With high sequencing depth and coverage, we constructed a single-cell transcriptional atlas of blood cells (ABC) on the basis of both protein-coding genes and long noncoding RNAs (lncRNAs), and showed a high consistence between them. Notably, putative lncRNAs and transcription factors regulating hematopoietic cell differentiation were identified. While common transcription factor regulatory networks were activated in neutrophils and monocytes, lymphoid cells dramatically changed their regulatory networks during differentiation. Furthermore, we showed a subset of nucleated erythrocytes actively expressing immune signals, suggesting the existence of erythroid precursors with immune functions. Finally, a web portal offering transcriptome browsing and blood cell type prediction has been established. Thus, our work provides a transcriptional map of human blood cells at single-cell resolution, thereby offering a comprehensive reference for the exploration of physiological and pathological hematopoiesis.

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Section: Discussionmentioning

confidence: 99%

Single-cell transcriptomic landscape of human blood cells

Xie

Liu

Zhang

et al. 2020

National Science Review

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“…Nucleated red blood cells (RBCs) of fish contain the transcriptional and translational machinery necessary to produce characteristic molecules of the immune system to respond against various infectious agents and play an active role in maintaining homeostasis of the fish immune system [95]. The nucleated RBC are reportedly involved in both innate and adaptive immune responses in fish [96]. Nucleated RBCs are able to phagocytose, acts as antigen-presenting cells [97,98], recognizes pathogen associated molecular pattern (PAMPs) by specific pathogen recognition receptors (PRRs), modulate leukocyte activity, release cytokine-like factors [99,100] and also induces interferon in fish [101].…”

Section: Red Blood Cellsmentioning

confidence: 99%

Immune System of Fish: An Evolutionary Perspective

Sahoo¹,

Banu²,

Prakash³

et al. 2021

Antimicrobial Immune Response

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Fishes are the most successful and diverse group of vertebrate animals, first appeared during Devonian period. Despite of certain differences, the immune system of fish is physiologically similar to that of higher vertebrates. The heterogenous group of fishes are the apparent link between innate immunity and the first appearance of the adaptive immune response. Importantly, fishes have immune organs homologous to that of mammalian immune system. In comparison to higher vertebrates, fishes live free in their environment from the early embryonic stage and during that time mostly they are dependent on non-specific immune system for their survival. In the fishes, non-specific immunity is the fundamental defense mechanism, therewith acquired immunity also plays key role in maintaining homeostasis by activation though a system of receptors proteins, which identify pathogen associated molecular pattern typical of pathogenic microorganism includes lipopolysaccharides, peptidoglycans, DNA, RNA and other molecules that are typically not present on the surface of multicellular organism. There are several external factors like environmental factors, biological factors, stress and internal factors like genetic makeup, age and sex, maternal effect etc. can affect immunological defense capabilities of the fishes.

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“…Avian nucleated red blood cells (RBCs) contain transcriptional and translational requirements for producing the characteristic molecules of the immune system to defend against pathogenic attacks. Therefore, nucleated RBCs seem to be involved in the regulation of both innate and adaptive immune responses, which highlights the crucial role of RBCs in host defence against pathogens [ 19 ]. To the best of our knowledge, there are no reports on gene expression in peripheral blood cells in nematode-infected chickens with different genetic backgrounds.…”

Section: Introductionmentioning

confidence: 99%

Differentially Expressed Gene Patterns in Ascarid-Infected Chickens of Higher- or Lower-Performing Genotypes

Kilic

Stehr

Hennies³

et al. 2021

Animals

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Here, we describe the first transcriptomic investigation of the peripheral blood of chickens exposed to Ascaridia galli and Heterakis gallinarum infections. We investigated differentially expressed gene (DEG) patterns in two chicken genotypes with either a higher (Lohmann Brown Plus, LB) or lower (Lohmann Dual, LD) laying performance level. The hens were experimentally coinfected with A. galli and H. gallinarum, and their worm burdens and infection parameters were determined six weeks post infection. Based on most representative infection parameters, the hens were clustered into lower- and higher-infection intensity classes. We identified a total of 78 DEGs contributing to infection-related phenotypic variation in the two genotypes. Our data showed significant upregulation of Guanylate Binding Protein 7 (GBP7) in LD hens, making it a promising candidate for tolerance to ascarid infections in chickens. Gene ontology analysis revealed higher transcriptome activity related to biological processes such as “response to external stimulus” in LB hens, implying a higher stress response in this genotype. In contrast, LD hens showed higher transcriptomic expression of genes related to ontology classes that are possibly associated with a higher tolerance to infections. These findings may help explain why lower-performing genotypes (i.e., LD) are less sensitive to infections in terms of maintaining their performance.

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Nucleated Red Blood Cells Contribute to the Host Immune Response Against Pathogens

Cited by 9 publications

References 74 publications

Single-cell transcriptomic landscape of human blood cells

Single-cell transcriptomic landscape of human blood cells

Immune System of Fish: An Evolutionary Perspective

Differentially Expressed Gene Patterns in Ascarid-Infected Chickens of Higher- or Lower-Performing Genotypes

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