Nucleic Acid and Protein Profile of Bacteriophages that Infect Pseudomonas syringae pv. glycinea, Bacterial Blight on Soybean

Addy, Hardian Susilo; Wahyuni, Wiwiek Sri

doi:10.1016/j.aaspro.2016.02.166

Cited by 5 publications

(4 citation statements)

References 28 publications

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“…Several phages infecting Psg have been previously reported in Refs. [ 15 , 16 , 17 ], although their description has been limited to their biological properties. Complete genome sequencing is an affordable and useful step for characterising candidate bacteriophages.…”

Section: Discussionmentioning

confidence: 99%

“…Some phage-based products are applied commercially against bacterial phytopathogens. Several phages that are effective against Psg have been described in the literature [ 15 , 16 , 17 ], although in-depth genomic analysis of the phages is missing in these publications and their ex vivo efficacy against the bacterial blight of plants has not been studied. The present research aims to fill some gaps in knowledge of the basic aspects of phage control of soy bacterial blight caused by Psg.…”

Section: Introductionmentioning

confidence: 99%

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Bacteriophage Control of Pseudomonas savastanoi pv. glycinea in Soybean

Tarakanov

Lukianova

Evseev

et al. 2022

Plants

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Bacterial viruses (bacteriophages) have been considered as potential agents for the biological control of bacterial phytopathogens due to their safety and host specificity. Pseudomonas savastanoi pv. glycinea (Psg) is a causative agent of the bacterial spotting of soybean (Glycine max Willd). The harm caused by this bacterium to crop production and the development of antibiotic resistance in Psg and other pathogenic microorganisms has led to the pursuit of alternative management strategies. In this study, three Psg-specific lytic bacteriophages were isolated from soybean field soil in geographically distant regions of Russia, and their potential for protective action on plants was assessed. Sequencing of phage genomes has revealed their close relatedness and attribution to the genus Ghunavirus, subfamily Studiervirinae, family Autographiviridae. Extensive testing of the biological properties of P421, the representative of the isolated phage group, has demonstrated a relatively broad host range covering closely related Pseudomonas species and stability over wide temperature (4–40 °C) and pH (pH 4–7) ranges, as well as stability under ultraviolet irradiation for 30 min. Application of the phages to prevent, and treat, Psg infection of soybean plants confirms that they are promising as biocontrol agents.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Bacteriophage Control of Pseudomonas savastanoi pv. glycinea in Soybean

Tarakanov

Lukianova

Evseev

et al. 2022

Plants

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“…In 2016, Addy and Wahyuni carried out characterization of phages ϕSK2a, ϕSK2b, ϕSK2c, and ϕMGX1, which showed the ability to generate lysis at 1 × 10 12 PFU/mL in P. syringae pv. glycinea, which causes diseases in soybeans and other legumes (Table 1) [80]. For the control of bacterial brown spot, studies have been carried out using Pseudomonas phage phi6.…”

Section: Bacteriophages In the Biocontrol Of Pseudomonas Sppmentioning

confidence: 99%

Phage Therapy for Crops: Concepts, Experimental and Bioinformatics Approaches to Direct Its Application

Villalpando-Aguilar

Matos-Pech

López-Rosas

et al. 2022

IJMS

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Phage therapy consists of applying bacteriophages, whose natural function is to kill specific bacteria. Bacteriophages are safe, evolve together with their host, and are environmentally friendly. At present, the indiscriminate use of antibiotics and salt minerals (Zn2+ or Cu2+) has caused the emergence of resistant strains that infect crops, causing difficulties and loss of food production. Phage therapy is an alternative that has shown positive results and can improve the treatments available for agriculture. However, the success of phage therapy depends on finding effective bacteriophages. This review focused on describing the potential, up to now, of applying phage therapy as an alternative treatment against bacterial diseases, with sustainable improvement in food production. We described the current isolation techniques, characterization, detection, and selection of lytic phages, highlighting the importance of complementary studies using genome analysis of the phage and its host. Finally, among these studies, we concentrated on the most relevant bacteriophages used for biocontrol of Pseudomonas spp., Xanthomonas spp., Pectobacterium spp., Ralstonia spp., Burkholderia spp., Dickeya spp., Clavibacter michiganensis, and Agrobacterium tumefaciens as agents that cause damage to crops, and affect food production around the world.

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“…glycinea and P. savastanoi pv. phaseolicola, respectively (Addy & Wahyuni, 2016;Marques & Samson, 2016).…”

Section: Introductionmentioning

confidence: 99%

Detection and characterization of endophytic bacteria causing knot in young olive trees

Khezri

Ghasemi²,

Ahangaran³

2019

AAS

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Olive knot is an important disease in most countries where olives are commercially grown. In the spring of 2015, some galls were observed on the trunk and branches of 4-year-old olive trees in the north of Iran. The bacteria were isolated from galls and all isolates were gram-negative, aerobic, and capable of producing florescent pigment. Other phenotypic characteristics of the isolates were assessed. Pathogenicity tests were carried out on olive branches incubated with different isolates. Primary symptoms were observed after two weeks. Sequences of 16S rRNA and RNA polymerase beta subunit genes of pathogenic isolates were completely similar to Pseudomonas savastanoi pv. savastanoi (Smith 1908) Young et al. 1978 in GenBank. Based on the results from phenotypic analyses, pathogenicity tests and phylogenetic data, the isolates were identified as P. savastanoi pv. savastanoi. The host range of our isolates was specific to olive trees. None of the inoculated oleander (Nerium oleander L.), winter jasmine (Jasminum nudiflorum <a title="John Lindley" href="https://en.wikipedia.org/wiki/John_Lindley">Lindl.</a>), Japanese privet (Ligustrum japonicum Thunb.) and ash (Fraxinus excelsior L.) developed disease symptoms. No difference in disease resistance was observed between six studied olive cultivars. There was no olive tree or orchard around the studied orchard as far as more than one kilometer. As the disease agent listed in Iran’s foreign quarantine pests and diseases list, appropriate quarantine and phytosanitary measures were undertaken to eradicate the disease.

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Nucleic Acid and Protein Profile of Bacteriophages that Infect Pseudomonas syringae pv. glycinea, Bacterial Blight on Soybean

Cited by 5 publications

References 28 publications

Bacteriophage Control of Pseudomonas savastanoi pv. glycinea in Soybean

Bacteriophage Control of Pseudomonas savastanoi pv. glycinea in Soybean

Phage Therapy for Crops: Concepts, Experimental and Bioinformatics Approaches to Direct Its Application

Detection and characterization of endophytic bacteria causing knot in young olive trees

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