Nucleic acid aptamers as aptasensors for plant biology

Tungsirisurp, Sireethorn; O’Reilly, Rachel K.; Napier, Richard

doi:10.1016/j.tplants.2022.10.002

Cited by 11 publications

(4 citation statements)

References 90 publications

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“…The G-quadruplex (G4) structure is an extremely stable quadruple-stranded DNA and RNA secondary structure held together by non-canonical guanine base pairs [27]. To further improve the stability of CSSD9, we added G4 to both ends of the sequence when designing the cyclic single-stranded DNA.…”

Section: Discussionmentioning

confidence: 99%

MSC microvesicles loaded G-quadruplex-enhanced circular single-stranded DNA-9 inhibits tumor growth by targeting MDSCs

Han,

Qin,

Zheng

et al. 2024

J Nanobiotechnol

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Background Myeloid-derived suppressor cells (MDSCs) promote tumor growth, metastasis, and lead to immunotherapy resistance. Studies revealed that miRNAs are also expressed in MDSCs and promote the immunosuppressive function of MDSCs. Currently, few studies have been reported on inducible cellular microvesicle delivery of nucleic acid drugs targeting miRNA in MDSCs for the treatment of malignant tumors. Results and conclusion In this study, we designed an artificial DNA named G-quadruplex-enhanced circular single-stranded DNA-9 (G4-CSSD9), that specifically adsorbs the miR-9 sequence. Its advanced DNA folding structure, rich in tandem repeat guanine (G-quadruplex), also provides good stability. Mesenchymal stem cells (MSCs) were prepared into nanostructured vesicles by membrane extrusion. The MSC microvesicles-encapsulated G4-CSSD9 (MVs@G4-CSSD9) was delivered into MDSCs, which affected the downstream transcription and translation process, and reduced the immunosuppressive function of MDSCs, so as to achieve the purpose of treating melanoma. In particular, it provides an idea for the malignant tumor treatment. Graphical Abstract

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Section: Discussionmentioning

confidence: 99%

MSC microvesicles loaded G-quadruplex-enhanced circular single-stranded DNA-9 inhibits tumor growth by targeting MDSCs

Han,

Qin,

Zheng

et al. 2024

J Nanobiotechnol

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“…However, it must be highlighted that engineering phytohormone signalling pathways is challenging and delicate, due to the large genetic redundancy and complexity of the signalling pathways ( Braguy and Zurbriggen, 2016 ). Nonetheless, we envision that the increased and accelerated adoption of advanced Synbio tools such as the genetically encoded phytohormone signaling manipulators (GEPHMans) ( Waadt, 2020 ), synthetic hormone reporters and biosensors ( Zhao et al., 2021 ), genetically encoded aptamers and heterologous systems ( Tungsirisurp et al., 2023 ), coupled with modelling tools, will simplify spatial and temporal monitoring of phytohormones and other analytes such as sugars, helping in the precise identification, analysis and regulation of interconnectivities between multiple pathways ( Isoda et al., 2021 ).…”

Section: Key Pathways Targeted For Manipulationmentioning

confidence: 99%

“…Therefore, each targeted modification will require regulators to monitor metabolic flux changes and associated downstream effects. Promisingly, as already highlighted, increased adoption of GEPHMans, synthetic biosensors, and heterologous systems, together with modelling tools, will simplify spatio-temporal monitoring of metabolic flux changes and aid precise identification, analysis and regulation of several crosstalking pathways ( Waadt, 2020 ; Isoda et al., 2021 ; Zhao et al., 2021 ; Tungsirisurp et al., 2023 ). Moreover, these Synbio-based tools have opened up prospects for multiple-traits or pathways modification and creation of novel plant systems custom-designed for specific climate environments ( Batista-Silva et al., 2020 ; Zhu et al., 2020 ; Shelake et al., 2022 ; Zhu et al., 2022b ).…”

Section: Perspectives On Metabolic Engineering Targeted Approaches As...mentioning

confidence: 99%

Metabolic pathways engineering for drought or/and heat tolerance in cereals

Liu,

Zenda,

Tian

et al. 2023

Front. Plant Sci.

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Drought (D) and heat (H) are the two major abiotic stresses hindering cereal crop growth and productivity, either singly or in combination (D/+H), by imposing various negative impacts on plant physiological and biochemical processes. Consequently, this decreases overall cereal crop production and impacts global food availability and human nutrition. To achieve global food and nutrition security vis-a-vis global climate change, deployment of new strategies for enhancing crop D/+H stress tolerance and higher nutritive value in cereals is imperative. This depends on first gaining a mechanistic understanding of the mechanisms underlying D/+H stress response. Meanwhile, functional genomics has revealed several stress-related genes that have been successfully used in target-gene approach to generate stress-tolerant cultivars and sustain crop productivity over the past decades. However, the fast-changing climate, coupled with the complexity and multigenic nature of D/+H tolerance suggest that single-gene/trait targeting may not suffice in improving such traits. Hence, in this review-cum-perspective, we advance that targeted multiple-gene or metabolic pathway manipulation could represent the most effective approach for improving D/+H stress tolerance. First, we highlight the impact of D/+H stress on cereal crops, and the elaborate plant physiological and molecular responses. We then discuss how key primary metabolism- and secondary metabolism-related metabolic pathways, including carbon metabolism, starch metabolism, phenylpropanoid biosynthesis, γ-aminobutyric acid (GABA) biosynthesis, and phytohormone biosynthesis and signaling can be modified using modern molecular biotechnology approaches such as CRISPR-Cas9 system and synthetic biology (Synbio) to enhance D/+H tolerance in cereal crops. Understandably, several bottlenecks hinder metabolic pathway modification, including those related to feedback regulation, gene functional annotation, complex crosstalk between pathways, and metabolomics data and spatiotemporal gene expressions analyses. Nonetheless, recent advances in molecular biotechnology, genome-editing, single-cell metabolomics, and data annotation and analysis approaches, when integrated, offer unprecedented opportunities for pathway engineering for enhancing crop D/+H stress tolerance and improved yield. Especially, Synbio-based strategies will accelerate the development of climate resilient and nutrient-dense cereals, critical for achieving global food security and combating malnutrition.

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“…Moreover, aptamers can be easily functionalized onto various sensing platforms, including optical, electrochemical, microfluidic, and surface plasmon resonance platforms. Aptasensors exploit aptamers, RNA, or single‐stranded DNA (ssDNA) molecules with high affinity for specific biotargets such as mycotoxin (Chen, Li et al., 2022; Tungsirisurp et al., 2022). These sensors offer advantages like rapid results, high sensitivity, and miniaturization capability.…”

Section: Introductionmentioning

confidence: 99%

Upconversion nanoparticles‐modified aptasensors for highly sensitive mycotoxin detection for food quality and safety

Bahari,

Mousavi Khaneghah,

Eş

2024

Comp Rev Food Sci Food Safe

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Mycotoxins, highly toxic and carcinogenic secondary metabolites produced by certain fungi, pose significant health risks as they contaminate food and feed products globally. Current mycotoxin detection methods have limitations in real‐time detection capabilities. Aptasensors, incorporating aptamers as specific recognition elements, are crucial for mycotoxin detection due to their remarkable sensitivity and selectivity in identifying target mycotoxins. The sensitivity of aptasensors can be improved by using upconversion nanoparticles (UCNPs). UCNPs consist of lanthanide ions in ceramic host, and their ladder‐like energy levels at f‐orbitals have unique photophysical properties, including converting low‐energy photons to high‐energy emissions by a series of complex processes and offering sharp, low‐noise, and sensitive near‐infrared to visible detection strategy to enhance the efficacy of aptasensors for novel mycotoxin detection. This article aims to review recent reports on the scope of the potential of UCNPs in mycotoxin detection, focusing on their integration with aptasensors to give readers clear insight. We briefly describe the upconversion photoluminescence (UCPL) mechanism and relevant energy transfer processes influencing UCNP design and optimization. Furthermore, recent studies and advancements in UCNP‐based aptasensors will be reviewed. We then discuss the potential impact of UCNP‐modified aptasensors on food safety and present an outlook on future directions and challenges in this field. This review article comprehensively explains the current state‐of‐the‐art UCNP‐based aptasensors for mycotoxin detection. It provides insights into potential applications by addressing technical and practical challenges for practical implementation.

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Nucleic acid aptamers as aptasensors for plant biology

Cited by 11 publications

References 90 publications

MSC microvesicles loaded G-quadruplex-enhanced circular single-stranded DNA-9 inhibits tumor growth by targeting MDSCs

MSC microvesicles loaded G-quadruplex-enhanced circular single-stranded DNA-9 inhibits tumor growth by targeting MDSCs

Metabolic pathways engineering for drought or/and heat tolerance in cereals

Upconversion nanoparticles‐modified aptasensors for highly sensitive mycotoxin detection for food quality and safety

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