Nucleic acid-based electrochemical nanobiosensors

Abi, Alireza; Mohammadpour, Zahra; Zuo, Xiaolei; Safavi, Afsaneh

doi:10.1016/j.bios.2017.11.019

Cited by 87 publications

(47 citation statements)

References 131 publications

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“…To enhance electroanalysis sensitivity, chemical modification of working electrodes with nanostructured carbon materials such as carbon nanotubes (CNTs) and, in particular, multi-walled CNTs (MWCNTs), graphene, ordered mesoporous carbon, or carbon nanofibers, is widely used [17][18][19]. Besides, commercial availability MWCNTs imparts the modified electrodes a number of beneficial properties, e.g., increased surface area, improved conductivity, and an expanded potential window.…”

Section: Introductionmentioning

confidence: 99%

Long-term stable poly(ionic liquid)/MWCNTs inks enable enhanced surface modification for electrooxidative detection and quantification of dsDNA

Sigolaeva

Булко

Kozin

et al. 2019

Polymer

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This work demonstrates the use of imidazolium-based poly(ionic liquid)s (PILs) as efficient dispersants of multi-walled carbon nanotubes (MWCNTs). With these polymeric dispersants, highly stable fine dispersions of MWCNTs (inks) can be easily prepared in aqueous media and applied for rather simple but efficient surface modification of screen-printed electrodes (SPEs). Such a modification of SPEs remarkably increases the electroactive surface area and accelerates the electron transfer rate due to synergistic combination of specific features of MWCNTs such as strong adsorptive property and high specific surface with the advantages of PILs like ion conductivity and dispersability. We further show that the PIL/MWCNT-modified SPEs can be beneficially utilized for direct electrochemical analysis of double stranded DNA (dsDNA). Specifically, it is exemplified by the direct electrooxidation of guanine and adenine bases in salmon testes dsDNA chosen as a model system. The linear ranges for the determination of dsDNA correspond to 5-500 µg/mL for the oxidative peak of guanine and 0.5-50 µg/mL for the oxidative peak of adenine. This makes direct electrochemical dsDNA detection with the use of the easy-preparable PIL/MWCNT-modified SPEs strongly competing to currently applied spectral and fluorescent techniques. Furthermore, we show that the developed constructs are capable of sensing a single point mutation in the 12-bases single-stranded DNA fragments. Such detection is of high clinical significance in choosing an adequate anticancer treatment, where the electrochemical identification of the point mutation could offer time and cost benefits.

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Section: Introductionmentioning

confidence: 99%

Long-term stable poly(ionic liquid)/MWCNTs inks enable enhanced surface modification for electrooxidative detection and quantification of dsDNA

Sigolaeva

Булко

Kozin

et al. 2019

Polymer

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“…Although electrical aptasensors offer advantages such as simplicity, low cost, good sensitivity and selectivity, at present, their use in clinical laboratories remains limited [29]. A comprehensive review about the recent progress and challenges in designing electrochemical aptasensors, their effectiveness in protein disease biomarkers or contaminants detection in food and the environment is reported in [30][31][32][33].…”

Section: Aptamers In Electrical Nanobiosensorsmentioning

confidence: 99%

Modelling and Development of Electrical Aptasensors: A Short Review

2018

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Aptamers are strands of DNA or RNA molecules, chemically synthetized and able to bind a wide range of targets, from small molecules to live cells, and even tissues, with high affinity and specificity. Due to their efficient targeting ability, they have many different kinds of applications. Particularly attractive is their use in biotechnology and disease therapy, in substitution of antibodies. They represent a promising way for early diagnosis (aptasensors), but also for delivering imaging agents and drugs and for inhibiting specific proteins (therapeutic aptamers). Starting by briefly reviewing the most recent literature concerning advances in biomedical applications of aptamers and aptasensors, the focus is on the issues of a theoretical/computational framework (proteotronics) for modelling the electrical properties of biomolecules. Some recent results of proteotronics concerning the electrical, topological and affinity properties of aptamers are reviewed.

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“…It has been proved that, nucleic acid (DNA/RNA) detection and analysis is highly essential not only for obtaining genetic information but also for the sake of diagnosis, identification and classification of various diseases and genetic disorders (Abi et al 2018;Zhai et al 1797 Generally, in the nucleic acid ECBs, sensor probe (recognition platform) consists of ssDNA or ssRNA covalently self-assembled onto the electrode surface, which should be conductive, biocompatible and have a low over potential (Odenthal and Gooding 2007). The probe sensor is then immersed into the solution containing target ssDNA/ssRNA, which is complementary to the probe strand, to form a double stranded nucleic acid helix.…”

Section: Dna-based Electrochemical Biosensor For Oligonucleotide Detementioning

confidence: 99%

Fabrication of Electrochemical-Based Bioelectronic Device and Biosensor Composed of Biomaterial-Nanomaterial Hybrid

Park

Min

Sohn

et al. 2018

Advances in Experimental Medicine and Biology

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Nucleic acid-based electrochemical nanobiosensors

Cited by 87 publications

References 131 publications

Long-term stable poly(ionic liquid)/MWCNTs inks enable enhanced surface modification for electrooxidative detection and quantification of dsDNA

Long-term stable poly(ionic liquid)/MWCNTs inks enable enhanced surface modification for electrooxidative detection and quantification of dsDNA

Modelling and Development of Electrical Aptasensors: A Short Review

Fabrication of Electrochemical-Based Bioelectronic Device and Biosensor Composed of Biomaterial-Nanomaterial Hybrid

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