Nucleic acid compartmentalization within the cell nucleus by in situ transferase‐immunogold techniques

Thiry, Marc

doi:10.1002/jemt.1070310103

Cited by 24 publications

(7 citation statements)

References 148 publications

(208 reference statements)

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“…As a control, the dihydrofolate reductase gene at chromosome 5q12-13 was compared with coiled bodies and was not found associated with this nuclear domain in any of the 34 nuclei analyzed. In agreement with reports that coiled bodies do not contain DNA (Thiry, 1994(Thiry, , 1995, we never found any of the genes colocalizing with coiled bodies. Now that we had established that the snRNA and histone genes were often associated with coiled bodies in T24 cells, they seemed good candidates to be involved with cleavage bodies adjacent to coiled bodies.…”

Section: Cleavage Bodies Coincide With Histone Genes But Not With Snrsupporting

confidence: 93%

“…The most well-known example is the nucleolus where genes coding for rRNA are organized in and around specific nuclear compartments that are enriched in essential transcription and processing factors (Hernandez-Verdun, 1986). Similarly, Xing et al (1993, 1995 have shown for several highly expressed genes that they are associated with the periphery of domains enriched in RNA polymerase II, polyadenylation, and splicing factors, known as nuclear speckles. For coiled bodies we have recently shown that they contain elevated levels of the transcription factors PTF and TBP, which are both essential for the transcription of the neighboring U1 and U2 snRNA genes (Schul et al, 1998b).…”

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confidence: 93%

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Nuclear Domains Enriched in RNA 3′-processing Factors Associate with Coiled Bodies and Histone Genes in a Cell Cycle–dependent Manner

Schul

Kraan

Matera

et al. 1999

MBoC

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Nuclear domains, called cleavage bodies, are enriched in the RNA 3Ј-processing factors CstF 64 kDa and and CPSF 100 kDa. Cleavage bodies have been found either overlapping with or adjacent to coiled bodies. To determine whether the spatial relationship between cleavage bodies and coiled bodies was influenced by the cell cycle, we performed cell synchronization studies. We found that in G1 phase cleavage bodies and coiled bodies were predominantly coincident, whereas in S phase they were mostly adjacent to each other. In G2 cleavage bodies were often less defined or absent, suggesting that they disassemble at this point in the cell cycle. A small number of genetic loci have been reported to be juxtaposed to coiled bodies, including the genes for U1 and U2 small nuclear RNA as well as the two major histone gene clusters. Here we show that cleavage bodies do not overlap with small nuclear RNA genes but do colocalize with the histone genes next to coiled bodies. These findings demonstrate that the association of cleavage bodies and coiled bodies is both dynamic and tightly regulated and suggest that the interaction between these nuclear neighbors is related to the cell cycle-dependent expression of histone genes. INTRODUCTIONThe cell nucleus contains various distinct structural and functional domains, each with its own morphology and protein composition Lamond and Earnshaw, 1998). One well-studied nuclear domain is the coiled body, which owes its name to its appearance as a ball of coiled threads in the electron microscope (Monneron and Bernhard, 1969). Coiled bodies are small spherical structures, one to five per nucleus, with a diameter of 0.2-1.0 m. They are evolutionary conserved from plants to mammals, indicating that they have a crucial role in the nucleus (for reviews, see Lamond and Carmo-Fonseca, 1993;Gall et al., 1995). Over the years, many nuclear factors have been found concentrated in coiled bodies. Among these are nucleolar constituents, such as fibrillarin (Raška et al., 1990) and Nopp140 (Meier and Blobel, 1992), U3 small nucleolar RNA (Jiménez-García et al., 1994), and nucleoplasmic factors, such as the general transcription factors TFIIH and TFIIF (Grande et al., 1997;Jordan et al., 1997) and the RNA-processing factors U1, U2, U4/U6, and U7 small nuclear ribonuclear protein (Carmo-Fonseca et al., 1992;Wu and Gall, 1993;Frey and Matera, 1995). The protein p80-coilin is especially enriched inside coiled bodies Raška et al., 1991) and is a hallmark for this nuclear domain. It has become clear that coiled bodies are dynamic structures. Disruption of cellular processes, e.g., by heat shock or drug treatment, rapidly alters the distribution and number of coiled bodies (Carmo-Fonseca et al., 1992). Coiled bodies also undergo changes during the cell cycle. During mitosis they disassemble, and only small remnants are left in the mitotic cell . Coilin enters the nucleus in telophase, but coiled bodies are not formed until later in G1 Ferreira et al., 1994). Thus several lines of investigation indicate that t...

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Section: Cleavage Bodies Coincide With Histone Genes But Not With Snrsupporting

confidence: 93%

mentioning

confidence: 93%

Nuclear Domains Enriched in RNA 3′-processing Factors Associate with Coiled Bodies and Histone Genes in a Cell Cycle–dependent Manner

Schul

Kraan

Matera

et al. 1999

MBoC

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“…The nucleolus is composed of four major ultrastructural components: (a) a fibrillar component where rRNA genes are transcribed; (b) a granular component composed of preribosomes and ribosomes at different stages of maturation; (c) a fibrillar center(s) or interphase nucleolar organizer region; and (d) nucleolar matrix and intranucleolar non-ribosomal chromatin (Schwarzacher and Wachtler 1993;Thiry 1995). Exposure of cells to anti-metabolites or cytotoxic drugs often results in segregation of the cell nucleolus into its components (Farber and Roberts 1971;Simard et al 1974).…”

Section: Discussionmentioning

confidence: 99%

Nucleolar Changes and Fibrillarin Redistribution Following Apatone Treatment of Human Bladder Carcinoma Cells

Jamison

Gilloteaux²,

Perlaky

et al. 2010

J Histochem Cytochem.

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(KS) S U M M A R Y Ascorbate and menadione (Apatone) in a ratio of 100:1 kills tumor cells by autoschizis. In this study, vitamin-induced changes in nucleolar structure were evaluated as markers of autoschizis. Human bladder carcinoma (T24) cells were overlain with vitamins or with culture medium. Supernatants were removed at 1-hr intervals from 1 to 4 hr, and the cells were washed with PBS and prepared for assay. Apatone produced marked alterations in nucleolar structure including redistribution of nucleolar components, formation of ringshaped nucleoli, condensation and increase of the proportion of perinucleolar chromatin, and the enlargement of nucleolar fibrillar centers. Immunogold labeling of the nucleolar rRNA revealed a granular localization in treated and sham-treated cells, and immunogold labeling of the rDNA revealed a shift from the fibrillar centers to the condensed perinucleolar chromatin. Fibrillarin staining shifted from the fibrillar centers and adjacent regions to a more homogeneous staining of the entire nucleolus and was consistent with the percentage of autoschizic cells detected by flow cytometry. Because autoschizis entails sequential reactivation of DNase I and DNase II, and because the fibrillarin redistribution following DNase I and Apatone treatment is identical, it appears that the nucleolar and fibrillarin changes are markers of autoschizis. (J Histochem Cytochem 58:635-651, 2010)

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“…A similar pattern of capping of nucleoli with CBs was also observed in primary human fibroblasts, when grown at 29C, and in HeLa cells exposed to transcription inhibitors (Carmo-Fonseca et al 1993). There exists, in addition, a considerable similarity between CBs and the dense fibrillar component of the nucleolus (Lafarga et al 1983(Lafarga et al ,1991Thiry 1995). Both exhibit equal and specific argentophilic properties (Seite et al 1982;Lafarga et al 1983;Berciano et al 1996).…”

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confidence: 99%

“…In fact, the presence of several Sm antigens in CBs , and of specific U-snRNAs (Thiry 1995), points to a potential role for CBs in pre-mRNA metabolism or in snRNP assembly or disassembly.…”

mentioning

confidence: 99%

Changes in Morphology and Spatial Position of Coiled Bodies during NGF-induced Neuronal Differentiation of PC12 Cells

Janevski

Park

Boni

1997

J Histochem Cytochem.

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SUMMARYInterphase nuclei are organized into structural and functional domains. The coiled body, a nuclear organelle of unknown function, exhibits cell type-specific changes in number and morphology. Its association with nucleoli and with small nuclear ribonucleoproteins (snRNPs) indicates that it functions in RNA processing. In cycling cells, coiled bodies are round structures not associated with nucleoli. In contrast, in neurons, they frequently present as nucleolar "caps." To test the hypothesis that neuronal differentiation is accompanied by changes in the spatial association of coiled bodies with nucleoli and in their morphology, PC12 cells were differentiated into a neuronal phenotype with nerve growth factor (NGF) and coiled bodies detected by immunocytochemical localization of p80-coilin and snRNPs. The fraction of cells that showed coiled bodies as nucleolar caps increased from 1.6 Ϯ 0.9% (mean Ϯ SEM) in controls to 16.5 Ϯ 1.6% in NGF-differentiated cultures. The fraction of cells with ring-like coiled bodies increased from 17.2 Ϯ 5.0% in controls to 57.8 Ϯ 4.4% in differentiated cells. This was accompanied by a decrease, from 81.2 Ϯ 5.7% to 25.7 Ϯ 3.1%, in the fraction of cells with small, round coiled bodies. SnRNPs remained associated with typical coiled bodies and with ring-like coiled bodies during NGF-induced recruitment of snRNPs to the nuclear periphery. Together with the observation that coiled bodies are also present as nucleolar caps in sensory neurons, the results indicate that coiled bodies alter their morphology and increase their association with nucleoli during NGF-induced neuronal differentiation.

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Nucleic acid compartmentalization within the cell nucleus by in situ transferase‐immunogold techniques

Cited by 24 publications

References 148 publications

Nuclear Domains Enriched in RNA 3′-processing Factors Associate with Coiled Bodies and Histone Genes in a Cell Cycle–dependent Manner

Nuclear Domains Enriched in RNA 3′-processing Factors Associate with Coiled Bodies and Histone Genes in a Cell Cycle–dependent Manner

Nucleolar Changes and Fibrillarin Redistribution Following Apatone Treatment of Human Bladder Carcinoma Cells

Changes in Morphology and Spatial Position of Coiled Bodies during NGF-induced Neuronal Differentiation of PC12 Cells

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