Nucleic Acid Extraction Methods and Technologies for On-Site Plant–Pathogen Diagnostics

Abstract

Global climate change has drastically affected agricultural production. Moreover, pandemics, wars, and rapid decreases in natural resources point to the emerging need for famine prevention by increasing smart agriculture and food production and decreasing food loss and waste. To achieve this, plant and food diseases should be detected early and rapidly treated. From this perspective, we focus on recent, simple, and instrument-free, nucleic acid extraction techniques, which are capable of isolating high-quality nucleic acids from plant tissues, to be either easily used in the field or simply integrated into various nucleic acid detection platforms or sensors.

Emerging Extraction and Diagnostic Tools for Detection of Plant Pathogens: Recent Trends, Challenges, and Future Scope

“…NA-based diagnostic tools, particularly those based on the PCR, are rapid, specific, and accurate tools. Plant pathologists understand that NA-based techniques are becoming more relevant for addressing applied concerns, and the use of these techniques has been explored over the years . However, efficient implementation of these approaches creates challenges, ranging from trained manpower, high cost of chemicals, and expensive equipment that necessitate a comprehensive understanding of the procedures of critical extraction associated with nucleic acids.…”

“…Plant pathologists understand that NA-based techniques are becoming more relevant for addressing applied concerns, and the use of these techniques has been explored over the years. 38 However, efficient implementation of these approaches creates challenges, ranging from trained manpower, high cost of chemicals, and expensive equipment that necessitate a comprehensive understanding of the procedures of critical extraction associated with nucleic acids. Therefore, several modern approaches for plant pathogen detection based on nucleic acids have been discussed below.…”