Nucleic acid hybridization for detection of herpes viruses in clinical specimens

Schuster, Volker; Matz, Bertfried; Wiegand, Helga; Polack, Axel; Corsten, Brigitte; Neumann‐Haefelin, Dieter

doi:10.1002/jmv.1890190310

Cited by 22 publications

(8 citation statements)

References 17 publications

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“…Therefore, the whole plasmid DNA probe was used throughout in this study. In this connection, Schuster et al [1986a] reported that gel impurities used for the isolation of restriction enzyme-digested DNA fragment and contaminated during electrophoresis for separating DNA, might decrease the nucleotide incorpora-tion rate in nick translation, and thereby prevent the increase in sensitivity of the assay.…”

Section: Discussionmentioning

confidence: 99%

Detection of herpes simplex virus type 1 in herpetic ocular diseases by DNA‐DNA hybridization using a biotinylated DNA probe

Nago¹,

Hayashi²,

Ochiai³

et al. 1988

Journal of Medical Virology

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A diagnostic hybridization assay for detecting herpes simplex virus type 1 (HSV-1) in ocular specimens was developed using cloned viral DNA as a probe. This hybridization assay is based on visualizing a biotinylated probe that is hybridized to the target DNA by a streptavidin/alkaline phosphatase system. The time required for performing this assay system is only two days. This assay system could detect a probe which had been hybridized to as little as 1 pg of homologous DNA and did not cross-react with DNA of other human herpes viruses except that of herpes simplex virus type 2 (HSV-2) which showed weak cross-reactivity. The assay system was applied to experimental keratitis in albino rabbits and clinical specimens. In experimental keratitis in rabbits it was possible to detect HSV-1 DNA in the eye swab samples at least until the ninth day after virus inoculation. Five clinical specimens collected from patients with corneal ulcer or blepharitis contained HSV-1 DNA in spite of the failure of demonstration of viral antigen and/or virus isolation in two cases.

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Section: Discussionmentioning

confidence: 99%

Detection of herpes simplex virus type 1 in herpetic ocular diseases by DNA‐DNA hybridization using a biotinylated DNA probe

Nago¹,

Hayashi²,

Ochiai³

et al. 1988

Journal of Medical Virology

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“…Other agents for which nucleic acid probes have been developed and analyzed include viruses such as CMV (30,100,150,158), HSV (34,52,91,149), human papillomavirus (179), adenovirus (60,174), hepatitis B virus (18), Epstein-Barr virus (24), and HIV (65). Nucleic acid probes for bacteria such as Salmonella typhi (145), legionellae (40), Vibrio cholerae (167), and campylobacters (167) also have been developed.…”

Section: Application Of Nucleic Acid Probesmentioning

confidence: 99%

Clinical laboratory applications of monoclonal antibodies

Payne¹,

Marshall²,

Shockley³

et al. 1988

Clin Microbiol Rev

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Monoclonal antibody (MAb) technology is well recognized as a significant development for producing specific serologic reagents to a wide variety of antigens in unlimited amounts. These reagents have provided the means for developing a number of highly specific and reproducible immunological assays for rapid and accurate diagnosis of an extensive list of diseases, including infectious diseases. The impact that MAbs have had in characterizing infectious disease pathogens, as well as their current and future applications for use in clinical microbiology laboratories, is reviewed. In addition, the advantages (and disadvantages) of the use of MAbs in a number of immunoassays, such as particle agglutination, radioimmunoassays, enzyme-linked immunosorbent assays, immunofluorescent-antibody assays, and immunohistology, are explored, including the use of these reagents in novel test system assays. Also, nucleic acid probe technology is compared with the use of MAbs from the perspective of their respective applications in the diagnosis of infectious disease agents. There is no question that hybridoma technology has the potential to alter significantly the methods currently used in most clinical microbiology laboratories.

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“…For direct immunofluorescence, well prepared cellular smears are necessary. Standard DNA hybridization techniques, i.e., dot blot [Schuster et al, 1986;Vonsover et al, 19871, need radioactive equipment. Diagnosis by antibody detection is not possible in the early days of disease and is complicated by cross-reactions to herpes simplex virus (HSV).…”

Section: Introductionmentioning

confidence: 99%

Diagnosis of acute and latent varicella‐zoster virus infections using the polymerase chain reaction

Dlugosch

Eis‐Hübinger

Kleim

et al. 1991

Journal of Medical Virology

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A simplified assay for the diagnosis of varicella-zoster virus (VZV) infections based on the polymerase chain reaction (PCR) is described. Omitting the procedures for extraction and purification of DNA, the crude vesicle fluid materials were used for PCR. Moreover, hybridization was not necessary for detection of the amplification products because they were already visible after ethidium bromide staining of the electrophoresis gel. Results could therefore be obtained within one day. In comparison to virus isolation, PCR proved much more rapid, highly sensitive, and specific. DNA extraction and a double PCR assay with nested primers were necessary for detection of latent VZV infections in trigeminal and thoracic ganglia. The data suggest that the procedures described are universally applicable to several types of specimens dependent on the calculated amount of target DNA.

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Nucleic acid hybridization for detection of herpes viruses in clinical specimens

Cited by 22 publications

References 17 publications

Detection of herpes simplex virus type 1 in herpetic ocular diseases by DNA‐DNA hybridization using a biotinylated DNA probe

Detection of herpes simplex virus type 1 in herpetic ocular diseases by DNA‐DNA hybridization using a biotinylated DNA probe

Clinical laboratory applications of monoclonal antibodies

Diagnosis of acute and latent varicella‐zoster virus infections using the polymerase chain reaction

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