Nucleic Acid Sample Preparation for Food Traceability

Pereira, L. S.; Gomes, Sónia; Martins-Lopes, Paula

doi:10.1007/978-1-4939-3185-9_14

Cited by 1 publication

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“…Grapevine varietal identification is easily guaranteed with the use of nuclear molecular markers, namely, Simple Sequence Repeat (SSR) approved and supported by the International Organization of Vine and Wine (OIV;OIV, 2007). Although SSR markers have been used for food authenticity purposes several problems have arisen related to the DNA quality, a result of the extraction procedures (reviewed by Pereira, Gomes, & Martins-Lopes, 2016). When considering must/wine matrices the presence of large quantities of polyphenols, polysaccharides and proteins sometimes inhibit PCR reactions (Is ßçi, Yildirim, & Altindisli, 2014).…”

Section: Introductionmentioning

confidence: 99%

High Resolution Melting (HRM) applied to wine authenticity

et al. 2017

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Wine authenticity methods are in increasing demand mainly in Denomination of Origin designations. The DNA-based methodologies are a reliable means of tracking food/wine varietal composition. The main aim of this work was the study of High Resolution Melting (HRM) application as a screening method for must and wine authenticity. Three sample types (leaf, must and wine) were used to validate the three developed HRM assays (Vv1-705bp; Vv2-375bp; and Vv3-119bp). The Vv1 HRM assay was only successful when applied to leaf and must samples. The Vv2 HRM assay successfully amplified all sample types, allowing genotype discrimination based on melting temperature values. The smallest amplicon, Vv3, produced a coincident melting curve shape in all sample types (leaf and wine) with corresponding genotypes. This study presents sensitive, rapid and efficient HRM assays applied for the first time to wine samples suitable for wine authenticity purposes.

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Section: Introductionmentioning

confidence: 99%