Nucleic acid sensor for insecticide detection

Solanki, Pratima R.; Prabhakar, Nirmal; Pandey, Manoj Kumar; Malhotra, Bansi D.

doi:10.1002/jmr.888

Cited by 15 publications

(7 citation statements)

References 31 publications

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“…In the present studies, PANI has been electrochemically prepared in water using lithium perchlorate (LiClO

_{4}^{−}

) as dopant11 to improve the conductivity of matrix. The low lattice energy and completely dissociated ions (ClO

_{4}^{−}

) are free for conduction,12 providing tetra‐coordinate environment with PANI 13–14.…”

Section: Introductionmentioning

confidence: 99%

Horse radish peroxidase immobilized polyaniline for hydrogen peroxide sensor

Solanki¹,

Kaushik²,

Ansari³

et al. 2011

Polymers for Advanced Techs

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Horse radish peroxidase (HRP) has been electrochemically entrapped into perchlorate (ClO 4−) doped polyaniline (PANI) film deposited onto indium‐tin‐oxide (ITO) coated glass plate. This HRP‐PANI‐ClO 4−/ITO bioelectrode characterized using Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), UV‐Visible spectroscopy, cyclic voltammetry (CV), and electrochemical impedance spectroscopy (EIS) techniques has been utilized for estimation of hydrogen peroxide (H2O2). This H2O2 sensor exhibits response time of 5 s, linearity from 3 to 136 mM, sensitivity as 0.5638 µA mM−1 cm−2 with linear regression of 0.985. The value of the Michaelis–Menten constant (Km) has been obtained as 1.984 mM. Copyright © 2009 John Wiley & Sons, Ltd.

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“…In the present studies, PANI has been electrochemically prepared in water using lithium perchlorate (LiClO

_{4}^{−}

) as dopant11 to improve the conductivity of matrix. The low lattice energy and completely dissociated ions (ClO

_{4}^{−}

) are free for conduction,12 providing tetra‐coordinate environment with PANI 13–14.…”

Section: Introductionmentioning

confidence: 99%

Horse radish peroxidase immobilized polyaniline for hydrogen peroxide sensor

Solanki¹,

Kaushik²,

Ansari³

et al. 2011

Polymers for Advanced Techs

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“…Mannosylated aniline monomer was electrochemically polymerized to form manno-PANI on the ITO electrode in HEPES (pH=7.4) buffer as reported in neutral aqueous media (Chan et al, 1994; Nguyen et al, 1999; Solanki et al, 2008; Yue et al, 1991; Zhou et al, 2009). At pH (7.4), carbohydrates are uncharged and their bioactivities are maintained.…”

Section: Methodsmentioning

confidence: 99%

Glycosylated aniline polymer sensor: Amine to imine conversion on protein–carbohydrate binding

Wang

Sun

Vegesna

et al. 2013

Biosensors and Bioelectronics

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In this report, functionalized mannosylated aniline polymer (manno-PANI) was investigated as an electrochemical platform to study carbohydrate–protein interactions by exploiting the conductivity change of manno-PANI when the specific lectin binding occurs. A systematic study was performed to characterize the interconversion of polyaniline content (from amine to imine) in manno-PANI by UV–vis spectroscopy during its binding with concanavalin A (Con A). Both X-ray photoelectron spectrometry (XPS) and UV–vis results suggest that Con A binding with the manno-PANI film triggers the switching of amine functionalities in the polyaniline backbone, converting them to imine forms. Electrochemical impedance spectroscopy (EIS) was used to quantify the specific interactions between Con A and mannose by measuring the impedance change of manno-PANI film for the detection of Con A. A linear relationship between the impedance and Con A concentration was obtained, and the detection limit reaches to 0.12 nM Con A in a buffer solution (pH=7.4), whereas the addition of nonspecific control lectins to the same manno-PANI film gave very little impedance variations. Stability characterization of the manno-PANI film over 20 weeks shows a maximum drift of only 3% from the original signal. Thus, the uniquely constructed carbohydrate–PANI hybrid is a promising new carbohydrate recognition moiety for studying carbohydrate-protein interactions, presumably leading to a new electrochemical method for characterization of carbohydrate–protein interactions and carbohydrate-mediated intercellular recognitions.

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“…Solanki et al 141 have described a surface plasma resonant-based DNA biosensor for the detection of As(III). This sensor was fabricated using a selfassembled monolayer of β-merceptoethanol (MCE) deposited on a gold substrate.…”

Section: Flow-injection Analysismentioning

confidence: 99%

Metals in River Water

Crompton

2015

Determination of Metals in Natural Waters, Sediments and Soils

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Nucleic acid sensor for insecticide detection

Cited by 15 publications

References 31 publications

Horse radish peroxidase immobilized polyaniline for hydrogen peroxide sensor

Horse radish peroxidase immobilized polyaniline for hydrogen peroxide sensor

Glycosylated aniline polymer sensor: Amine to imine conversion on protein–carbohydrate binding

Metals in River Water

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