Nucleic acids and growth of Calanus finmarchicus in the laboratory under different food and temperature conditions

Wagner, Melissa; Campbell, Robert G.; Boudreau, Carla A.; Durbin, Edward G.

doi:10.3354/meps221185

Cited by 72 publications

(70 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The growth models developed for Mysis relicta differ from the growth models developed for other cructaceans. Nucleic acid-based models developed for lobsters, copepods and cladocerans were based on specific life stages or a particular size of individual (Juinio et al 1992, Saiz et al 1998, Wagner et al 2001, Vrede et al 2002. They successfully related SGR or egg production rate to RNA:DNA changes within that size class.…”

Section: Growth Models: Inclusion Of Nucleic Acid and Protein Indicesmentioning

confidence: 99%

“…Physiological correlates of growth, such as enzyme activity, lipid level and nucleic acid concentration or ratios (Clarke & Walsh 1993, Houlihan et al 1993, Bergeron 1997) may provide an alternate means of detecting changes. We chose to study nucleic acids because they are closely linked to the production of body mass and appear to change at a time frame of interest for studying species interactions (Foster 1990, Schlechtriem et al 2008 Growth rate and protein synthesis rates are often correlated with RNA content, or ratios of RNA:DNA and RNA:protein in both vertebrates and invertebrates (Buckley 1979, Houlihan et al 1993, Dahlhoff & Menge 1996, Bergeron 1997, Saiz et al 1998, Wagner et al 2001. While ribosomes, which are composed largely of ribosomal RNA (rRNA), set the upper capacity for protein synthesis, the actual rate will vary with translational efficiency depending on short-term needs (Millward et al 1973, Preedy et al 1988.…”

Section: Introductionmentioning

confidence: 99%

“…copepod: Euchaeta norvegica Boeck, 1872 (Bamstedt & Skjoldal 1980); Calanus sp. (Wagner et al 2001); mysid shrimp: Mysis relicta (Nordin 2005). In crustaceans, molting further complicates the interpretation of nucleic acid concentrations and ratios because they may follow distinct patterns through the molt cycle; e.g.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Relating nucleic acid and protein indices to growth in Mysis relicta: ration, cycling temperature, and metabolism

Johannsson¹,

Bowen²,

Wood³

et al. 2008

Aquat. Biol.

View full text Add to dashboard Cite

We investigated growth rate, nucleic acid (DNA, RNA) and protein indices and respiration in juvenile (8.5 to 12 mm total body length, 7 to 20 mg wet wt) and young adult (12 to 14 mm, 20 to 30 mg wet wt) Mysis relicta, as a function of temperature, body mass and molt stage in order to develop methods to assess condition or growth in the field. Mysids were exposed to either a preferred temperature (6.5°C) and 3 ration levels, or a range of constant and dielly-cycling (DC) temperatures with ad libitum feeding. Mysid growth parameters (specific rates of growth [SGR], respiration [M O 2 ], and RNA content cell -1 ) integrated the DC temperature experienced as averaged responses weighted by the time spent at each temperature. M O 2 peaked at 12.7°C on acute temperature exposure from 4.2°C. M O 2 compensation with prolonged temperature exposure occurred at mean diel temperatures ≤ 8.5°C. Mysids could not survive at 16°C even for 5 h d -1 . These results confirm behavioral observations of temperature preferences. RNA concentration in M. relicta increased with ration and decreasing temperatures. Protein:DNA ratio, %protein and SGR increased with ration and then plateaued. Protein:DNA ratio, %protein and DNA:weight ratio did not change with temperature with unlimited feeding. Forward, stepwise, multiple regression models for each experiment and the combined data accounted for 31 to 72% of variability in SGR. Our experimental data provide guidance, a preliminary temperature-correction factor for RNA, and benchmarks for use of nucleic acid and protein indices in assessing growth or condition of M. relicta in the field.

show abstract

Section: Growth Models: Inclusion Of Nucleic Acid and Protein Indicesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Relating nucleic acid and protein indices to growth in Mysis relicta: ration, cycling temperature, and metabolism

Johannsson¹,

Bowen²,

Wood³

et al. 2008

Aquat. Biol.

View full text Add to dashboard Cite

show abstract

“…Biochemical indices of physiological variables are based on key components of synthetic or metabolic pathways directly or indirectly linked to cell proliferation and synthesis of important biological macromolecules. In particular, concentration of ribonucleic acid (RNA) has been widely used as a sensitive indicator of the rate of copepod growth, both reproductive (Nakata et al 1994;Saiz et al 1998;Holmborn andGorokhova 2008a, 2008b) and somatic (Wagner et al 2001), and as a measure of metabolic activity in response to nutritional conditions (Wagner et al 1998;Gorokhova et al 2007) and environmental stress (Calliari et al 2006), with the RNA concentration generally being expressed as the ratio of RNA to DNA or as the ratio of RNA to protein. The rationale is that total RNA content in a somatic cell is primarily a function of ribosome number correlating with protein synthesis, whereas the amount of DNA is quasiconstant and, therefore, an indicator of the cell number; the RNA:DNA ratio is thus an indicator of the amount of RNA per cell.…”

Section: Introductionmentioning

confidence: 99%

Biochemical proxies for growth and metabolism in Acartia bifilosa (Copepoda, Calanoida)

Holmborn

Dahlgren

Holeton

et al. 2009

Limnology & Ocean Methods

View full text Add to dashboard Cite

Biochemical proxies are becoming increasingly common for growth assessment in zooplankton. Their suitability is often unknown, however, and proper calibration is lacking. We investigated correlations between physiological variables (ingestion, egg production, and respiration rates) and biochemical indices related to protein synthesis (RNA content, RNA:DNA ratio, RNA:protein ratio, and protein specific aminoacyl-tRNA synthetases [spAARS] activity) in copepods Acartia bifilosa exposed to different algal concentrations (0-1200 µg C L -1). All variables assayed increased with increasing food concentration either linearly (spAARS) or nonlinearly (all other variables). Egg production and ingestion rates were significantly and positively correlated with RNA content and RNA:protein ratio, whereas correlations with spAARS and RNA:DNA ratio were weaker or nonsignificant. However, when RNA:DNA ratio and spAARS activity were used as predictors of ingestion, together they had higher explanatory value than did either variable separately. As there were substantial differences in saturating food concentrations among the assayed variables, applicability of biomarkers as proxies of physiological rates will be more reliable if restricted to the nonsaturated phase of the functional response of either variable, unless both variables saturate simultaneously. These findings contribute to methodology of zooplankton growth assessment and to our understanding of biochemical processes underlying growth and metabolism in copepods.

show abstract

“…In marine field studies, significant correlations between chlorophyll a concentrations (indicator of food), copepod egg production and the copepod RNA:DNA ratios have been described (Nakata 1990;Nakata et al 1994;Laabir et al 1998;Saiz et al 1998). In laboratory studies C. finmarchicus RNA:DNA ratios are good indicators of food availability (Wagner et al 1998(Wagner et al , 2001.…”

Section: Introductionmentioning

confidence: 99%

Impacts of copepods on marine seston, and resulting effects on Calanus finmarchicus RNA:DNA ratios in mesocosm experiments

et al. 2004

View full text Add to dashboard Cite

We investigated the impact of copepods on the seston community in a mesocosm set-up, and assessed how the changes in food quantity, quality and size affected the condition of the grazers, by measuring the RNA:DNA ratios in different developmental stages of Calanus finmarchicus. Manipulated copepod densities did not affect the particulate carbon concentration in the mesocosms. On the other hand, chlorophyll a content increased with higher copepod densities, and increasing densities had a positive effect on seston food quality in the mesocosms, measured as C:N ratios and x3:x6 fatty acid ratios. These food quality indicators were significantly correlated to the nutritional status of C. finmarchicus. In contrast to our expectations, these results suggest a lower copepod growth potential on higher quality food. However, in concordance with earlier studies, we found that when copepods were in high densities the large particles (>1000 lm 3 ) decreased and that the smaller particles (<1000 lm 3 ) increased in number. These patterns were closely linked to the condition of C. finmarchicus, which were of better condition (RNA:DNA ratios) with increasing biovolumes of large particles, and, conversely, lower RNA:DNA ratios with increasing biovolumes of smaller particles. Consequentially, the selective grazing by copepods stimulated increased biovolumes of smaller plankton, and this increase was responsible for the increased food quality, in terms of C:N and x3:x6 ratios. Thus, we conclude that the decreasing growth potentials of C. finmarchicus were a result of a decrease of favourably sized food particles, induced by copepod grazing.

show abstract

Nucleic acids and growth of Calanus finmarchicus in the laboratory under different food and temperature conditions

Cited by 72 publications

References 36 publications

Relating nucleic acid and protein indices to growth in Mysis relicta: ration, cycling temperature, and metabolism

Relating nucleic acid and protein indices to growth in Mysis relicta: ration, cycling temperature, and metabolism

Biochemical proxies for growth and metabolism in Acartia bifilosa (Copepoda, Calanoida)

Impacts of copepods on marine seston, and resulting effects on Calanus finmarchicus RNA:DNA ratios in mesocosm experiments

Contact Info

Product

Resources

About