Nucleic acids and protein metabolism in barley seedlings IV. Translocation of ribonucleic acids

Ledoux, L; Huart, R.

doi:10.1016/0926-6550(62)90081-6

Cited by 8 publications

(6 citation statements)

References 8 publications

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“…The fact that nio net gain in ntucleic acid-P occturs in the entire plant is differenit from the restults obtaiined with corn bl) Ingle and Hlageman (11) and for wheat b))y AIatsuishita (14). However is similar to restults obtained for barley by Ledoulx and Huart (13) and for bean by Oota and Takota (18). These restults wouild then stuggest that either nucleic acids moved intact from the reserve tissule into the developing axis or that nucleic acid synthesis in the axis was solely at the expense of nitcleotides derived from the reserve nuicleic acids.…”

Section: Methodssupporting

confidence: 73%

“…Information on 3P or nucleotide incorporation into nucleotides and nucleic acids are needed to clarify this point. From the variable reports in the literature (4,7,11,13,14,17,18), however, it wouild appear that some species simply possess the capacity for a net synthesis of nutcleic acids duiring dark germination while others do not.…”

Section: Discussionmentioning

confidence: 99%

“…This is especially stuggeste(d in the case of ntucleic acid (figs 10,14). WVhether the ntucleic acids move as macromolecules (13,17,18) or are first degraded and resynthesized in the embryonic axis (4, 7,1 1, 14) is impossible to ascertain from this typo of data. Information on 3P or nucleotide incorporation into nucleotides and nucleic acids are needed to clarify this point.…”

Section: Discussionmentioning

confidence: 99%

“…This stems primarily from the uncertainty of the natture of the mobilization, transfer and tutilization of other reserve phosphate containing compouinds. For example, some investigators believe that ntucleic acids are transported from reserve tissule to axis intact or as the nticleotides (13,17,18) while others feel that some de novo synthesis of nucleic acids does occur in the axis (4,7,11). Similarly, it is not c!ear whether phospholipids move from the reserve tissue to the axis or whether they are synthesized de novo in the axis, althotugh the latter appeared to be the case for cotton germination (5).…”

mentioning

confidence: 99%

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Phosphorus Metabolism of Germinating Oat Seeds

Hall

Hodges

1966

Plant Physiol.

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Summary. An investigation has been made of the changes in the major phosphorus containing stubstances in Avena sativa during the first 8 days of dark germination. The endosperm, roots, and shoots were analyzed separately for acid soluble-P, phytic acid-P, inorganic-P, lipid-P, nucleic acid-P, and protein-P. Phytic acid-P comprised 53 % of the total seed phosphate, while the sum of lipid-P, nucleic acid-P and protein-P comprised 27 % of the seed phosphate. All these reserve phosphate materials were mobilized and transferred to the developinig axis. The phosphate from phytic acid appeared almost entirely as inorganic-P in the roots and shoots. A close stoichiometry existed between the rate of loss of nucleic acid-P from the endosperm and its rate -Of appearance in the roots and shoots. Thus no net synthesis of nucleic acid occurred during the 8-day period examined. The rate of synthesis of lipid-P in the roots and shoots exceeded its rate of disappearance from the endosperm during the first 4 days of germination. Protein-P increased in the roots and shoots during germination, btit at a rate less than its rate of disappearance from the endosperm. The results provide a relatively complete description of the associated with germination of oats.The major metabolic processes associated with seed germination are the mobilization of storage materials in the reserve tissule and their subsequLent transfer to and utilization by the developing embryonic axis. This mobilization, transport, and ultilization has been studied for a variety of substances (cf. 15, 23), buit primarily has been concerned with the various N-containing suibstances suich as proteins and nucleic acids. In light of the importance of various phosphorylated substances in metabolism it seemed desirable to characterize the germinatioln process with respect to the time sequence of changes in the major phosphorylated substances. Althoulgh specific aspects of phosphoruis metabolism dturing germination have been examined such as changes in phytic acid, nucleic acid, phospholipids, etc. (1, 3, 11, 20) over-all aspects of phosphorus metabolism presuimably enters into various synthetic reactioniv occuirring in the developing axis. However, the extent of this inorganic-P uitilization during (lark germination for synthetic reactions is uinclear. This stems primarily from the uncertainty of the natture of the mobilization, transfer and tutilization of other reserve phosphate containing compouinds. For example, some investigators believe that ntucleic acids are transported from reserve tissule to axis intact or as the nticleotides (13, 17, 18) while others feel that some de novo synthesis of nucleic acids does occur in the axis (4,7,11). Similarly, it is not c!ear whether phospholipids move from the reserve tissue to the axis or whether they are synthesized de novo in the axis, althotugh the latter appeared to be the case for cotton germination (5). It has also been shown that phosphoproteins occuir itn seeds (5,12,24), however, their mobilization and utilization du...

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Section: Methodssupporting

confidence: 73%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

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Phosphorus Metabolism of Germinating Oat Seeds

Hall

Hodges

1966

Plant Physiol.

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“…On the contrary in the non-photosynthetic sprout rRNA is high (350 mg/s; fresh weight) and many ribosomal aggregates are present (Figure 4 A). This fact could suggest that, as found in the seeds (Barker and Douglas 1960, Ledoux and Huart 1962, Ingle and Hageman 1965 there is a probable translocation of protein and RNA materials from the tuber to the sprout. In this period, in fact, the sprout is not photosynthetic and it is dependent on the tuber reserves.…”

Section: Fractionsmentioning

confidence: 78%

Content and Aggregation of Ribosomes during Formation, Dormancy and Sprouting of Tubers of Helianthus tuberosus

Bagni

Donini

Fracassini

1972

Physiologia Plantarum

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The ribosomes and their qualitative (monosomes‐polysomes) and quantitative variations over a whole vegetative period of the tuber of Helianthus tuberosus L. (cv. OB 1) were examined. Tubers in different phases of growth, dormancy and sprouting or slices of dormant tubers activated with 2 x 10‐6M indol‐3‐acetic acid were used. The ribosomes were analyzed by a linear sucrose gradient. During flowering, polysomes of tuber disappeared almost completely and rRNA decreased in comparison with the level present at the beginning of tuber formation. After flowering, there was a new synthesis of monosomes and polysomes until the onset of dormancy; this last period was characterized by a marked increase in polysomes and a proportional increase in monosomes. The level remained almost constant till the break of dormancy. When the tubers sprouted, ribosomes, present almost exclusively as monosomes, decreased considerably; on the contrary the non‐photosynthetic sprouts contained many monosomes and polysomes. The first phases of activation (3 h) of tuber slices were characterized by a RNA synthesis, which occurred during one hour, in the subunit region of the gradient. Successively (10 h of activation) the 32P incorporation was seen also in the polysome region and increased with time. Some possible interpretations of these last results are discussed.

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