Nucleic Acids Chemistry and Engineering: Special Issue on Nucleic Acid Conjugates for Biotechnological Applications

Endoh, Tamaki; Rozners, Eriks; Ohtsuki, Takashi

doi:10.3390/app11083594

Cited by 1 publication

(1 citation statement)

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“…Synthetic oligonucleotides (ON), such as antisenses (ASOs), small interfering RNAs (siRNAs), or aptamers, not only support investigations of novel therapeutic approaches − but also pave the way for detection with high selectivity and sensitivity. − ON conjugates are currently used in a wide range of applications, including nanomaterials, structure analysis probes, and sensing technologies . They are also extensively studied as therapeutic agents, eliciting biological effects via different mechanisms. ,, In the field of functionalized materials, the use of hybrid polymer–ON conjugates is now widespread .…”

Section: Introductionmentioning

confidence: 99%

Electropolymerizable Thiophene–Oligonucleotides for Electrode Functionalization

Kassahun

Farias

Benizri

et al. 2022

ACS Appl. Mater. Interfaces

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Inserting complex biomolecules such as oligonucleotides during the synthesis of polymers remains an important challenge in the development of functionalized materials. In order to engineer such a biofunctionalized interface, a single-step method for the covalent immobilization of oligonucleotides (ONs) based on novel electropolymerizable lipid thiophene–oligonucleotide (L-ThON) conjugates was employed. Here, we report a new thiophene phosphoramidite building block for the synthesis of modified L-ThONs. The biofunctionalized material was obtained by direct electropolymerization of L-ThONs in the presence of 2,2′-bithiophene (BTh) to obtain a copolymer film on indium tin oxide electrodes. In situ electroconductance measurements and microstructural studies showed that the L-ThON was incorporated in the BTh copolymer backbone. Furthermore, the covalently immobilized L-ThON sequence showed selectivity in subsequent hybridization processes with a complementary target, demonstrating that L-ThONs can directly be used for manufacturing materials via an electropolymerization strategy. These results indicate that L-ThONs are promising candidates for the development of stable ON-based bioelectrochemical platforms.

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