Nucleocytoplasmic shuttling of the La motif-containing protein Sro9 might link its nuclear and cytoplasmic functions

Röther, Susanne; Burkert, Cornelia; Brünger, Katharina M.; Mayer, Andreas; Kieser, Anja; Sträßer, Katja

doi:10.1261/rna.2089110

Cited by 19 publications

(26 citation statements)

References 50 publications

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“…The integrity of the TAPtagged and slf1 deletion strain was verified by PCR. The strains SRO9-TAP MEX67 and SRO9-TAP mex67-5 have been described earlier (Rother et al 2010). Strains SLF1-TAP MEX67 (MATa;15;112; trp1-1; SLF1-TAPTTRP1-KL, mex67THIS3; pUN100-MEX67) and 15;112; trp1-1; SLF1-TAPTTRP1-KL, mex67THIS3; pUN100-mex67-5) were obtained by integration of the TAP-tag at the C-terminal end of the SLF1 ORF into the genome of the MEX67 shuffle strain by homologous recombination (Puig et al 2001).…”

Section: Methodsmentioning

confidence: 99%

“…The Slf1p paralog Sro9p is implicated in both transcription and translation (Sobel and Wolin 1999;Tan et al 2000) and has recently been shown to shuttle between the nucleus and the cytoplasm (Rother et al 2010). In order to elucidate the mode of Slf1p-mediated mRNA expression regulation, we first investigated the cellular localization of Slf1p under normal growth conditions and after inhibition of mRNA export, a condition in which Sro9p was shown to accumulate in the nucleus (Rother et al 2010). Slf1 was TAP-tagged in WT and isogenic mex67-5 cells, bearing a temperaturesensitive mutant allele of the mRNA export factor Mex67, which is thought to be the primary mRNA export factor in yeast.…”

Section: Slf1p Is Localized To the Cytoplasm And Affects The Decay Ofmentioning

confidence: 99%

“…The subcellular localization of Slf1p and Sro9p under conditions where mRNA export is inhibited was analyzed using strains SLF1-TAP MEX67, SLF1-TAP mex67-5, SRO9-TAP MEX67, and SRO9-TAP mex67-5 by indirect immunofluorescence according to the method described by Rother et al (2010).…”

Section: Western Blots and Immunofluorescencementioning

confidence: 99%

“…Sro9p has been implicated in golgi secretion (Tsukada and Gallwitz 1996), budding (Imai et al 1996), cytoskeleton formation (Kagami et al 1997), and RNAP II-mediated transcription (Tan et al 2000). Recently, it has been reported that Sro9p shuttles between the nucleus and the cytoplasm and that it associates with actively transcribed chromatin, supporting a model in which the RBP guides mRNAs through the process of gene expression, from nuclear transcription to cytoplasmic translation (Rother et al 2010). However, potential Sro9p mRNA targets have not been identified to date.…”

Section: Introductionmentioning

confidence: 96%

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La-motif–dependent mRNA association with Slf1 promotes copper detoxification in yeast

Schenk

Meinel²,

Sträßer³

et al. 2012

RNA

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The La-motif (LAM) is an ancient and ubiquitous RNA-binding domain defining a superfamily of proteins, which comprises the genuine La proteins and La-related proteins (LARPs). In contrast to La, which binds and stabilizes pre-tRNAs and other RNA polymerase III transcripts, data on function and RNA targets of the LARPs have remained scarce. We have undertaken a global approach to elucidate the previously suggested role of the yeast LARP Slf1p in copper homeostasis. By applying RNA-binding protein immunopurification-microarray (RIP-Chip) analysis, we show that Slf1p and its paralog Sro9p copurify with overlapping sets of hundreds of functionally related mRNAs, including many transcripts coding for ribosomal proteins and histones. Interestingly, among these potential RNA targets were also mRNAs coding for proteins critical for protection of cells against elevated copper concentrations. Mutations introduced in the conserved aromatic patch of the LAM in Slf1p drastically impaired both association with its targets and Slf1-mediated protection of cells against toxic copper concentrations. Furthermore, we show that Slf1p stabilizes copper-related mRNA targets in a LAM-dependent manner. These results provide the first evidence for post-transcriptional regulation of factors/pathways implicated in copper homeostasis by a cytoplasmic RBP.

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Section: Methodsmentioning

confidence: 99%

Section: Slf1p Is Localized To the Cytoplasm And Affects The Decay Ofmentioning

confidence: 99%

Section: Western Blots and Immunofluorescencementioning

confidence: 99%

Section: Introductionmentioning

confidence: 96%

See 2 more Smart Citations

La-motif–dependent mRNA association with Slf1 promotes copper detoxification in yeast

Schenk

Meinel²,

Sträßer³

et al. 2012

RNA

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“…The ChIP experiments were performed as in Rother et al (2010), and the ChIP-chip experiments were performed as in Meinel et al (2013). RPC25 and rpc25-S100P cells were grown for 10 h at 25°C or shifted to 37°C.…”

Section: Generation Of a Nab2 Temperature-sensitive Allelementioning

confidence: 99%

The poly(A)-binding protein Nab2 functions in RNA polymerase III transcription

2015

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RNA polymerase III (RNAPIII) synthesizes most small RNAs, the most prominent being tRNAs. Although the basic mechanism of RNAPIII transcription is well understood, recent evidence suggests that additional proteins play a role in RNAPIII transcription. Here, we discovered by a genome-wide approach that Nab2, a poly(A)-binding protein important for correct poly(A) tail length and nuclear mRNA export, is present at all RNAPIII transcribed genes. The occupancy of Nab2 at RNAPIII transcribed genes is dependent on transcription. Using a novel temperature-sensitive allele of NAB2, nab2-34, we show that Nab2 is required for the occupancy of RNAPIII and TFIIIB at target genes. Furthermore, Nab2 interacts with RNAPIII, TFIIIB, and RNAPIII transcripts. Importantly, impairment of Nab2 function causes an RNAPIII transcription defect in vivo and in vitro. Taken together, we establish Nab2, an important mRNA biogenesis factor, as a novel player required for RNAPIII transcription by stabilizing TFIIIB and RNAPIII at promoters.[Keywords: RNA polymerase III; Nab2; tRNA; ncRNA; gene expression; TFIIIB] Supplemental material is available for this article. RNA polymerase III (RNAPIII) is responsible for the production of most noncoding RNAs (ncRNAs) such as tRNAs, the 5S rRNA (encoded by RDN5), the RNA of the signal recognition particle (SCR1), the spliceosomal U6 snRNA (SNR6), and the RNA component of RNase P (RPR1). Transcription by RNAPIII is initiated at three classes of promoters, which are highly diverse, have a relatively small number of cis-acting elements, and require comparably few transcription factors (TFs) (White 2011;Orioli et al. 2012;Acker et al. 2013 and references therein). Type 1 promoters are exclusively present at 5S rRNA-encoding genes (RN5S in mammals, and RDN5 in yeast). Type 1 promoters have three internal elements: the A box, which is recognized by TFIIIC, and the intermediate element (IE) and the C box, which are recognized by TFIIIA. The most common RNAPIII promoters are of type 2 and drive transcription of tRNA genes as well as, e.g., the SCR1 and RPR1 genes. Type 2 promoters consist of A-and B-box elements, which together recruit TFIIIC. TFIIIC recruits TFIIIB to the transcription start site at type 1 and type 2 promoters. Type 3 promoters are relatively rare, and, in contrast to type 1 and 2 promoters, all of their elements lie 5 ′ of the transcription start site: the distal sequence element (DSE), the proximal sequence element (PSE), and the TATA box. The DSE recruits the TFs Oct1 and STAF, whereas the PSE recruits SNAP c , which in turn binds TFIIIB and recruits it together with the TATA box to the promoter (Schramm and Hernandez 2002 and references therein). Type 3 promoters are only present in vertebrates; e.g., at the U6 snRNA gene. The promoter of the Saccharomyces cerevisiae U6 snRNA gene SNR6 is of type 2 and contains an upstream TATA box in addition to the canonical A and B boxes (Brow and Guthrie 1990;Eschenlauer et al. 1993). At all three types of promoters, TFIIIB recruits RNAPIII. TFIIIB i...

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Genetic interaction between glyoxylate pathway regulator UCC1 and La‐motif‐encoding SRO9 regulates stress response and growth rate improvement in Saccharomyces cerevisiae

Pandita

Shoket

Rakewal

et al. 2021

J Biochem & Molecular Tox

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Nonavailability of glucose as a carbon source results in glyoxylate pathway activation, which metabolizes nonfermentable carbon for energy generation in Saccharomyces cerevisiae. Ucc1p of S. cerevisiae inhibits activation of the glyoxylate pathway by targeting Cit2p, a key glyoxylate enzyme for ubiquitin-mediated proteasomal degradation when glucose is available as a carbon source. Sro9p, a Lamotif protein involved in RNA biogenesis, interacts physically with the messenger RNA of UCC1; however, its functional relevance is yet to be discovered. This study presents binary epistatic interaction between UCC1 and SRO9, with functional implication on the growth rate, response to genotoxic stress, resistance to apoptosis, and petite mutation. Cells with ucc1Δsro9Δ, as their genetic background, exhibit alteration in morphology, improvement in growth rate, resistance to apoptosis, and petite mutation. Moreover, the study indicates a cross-link between ubiquitinproteasome system and RNA biogenesis and metabolism, with applications in industrial fermentation and screening for cancer therapeutics.

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Nucleocytoplasmic shuttling of the La motif-containing protein Sro9 might link its nuclear and cytoplasmic functions

Cited by 19 publications

References 50 publications

La-motif–dependent mRNA association with Slf1 promotes copper detoxification in yeast

La-motif–dependent mRNA association with Slf1 promotes copper detoxification in yeast

The poly(A)-binding protein Nab2 functions in RNA polymerase III transcription

Genetic interaction between glyoxylate pathway regulator UCC1 and La‐motif‐encoding SRO9 regulates stress response and growth rate improvement in Saccharomyces cerevisiae

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