Nucleolar Organizer Region Activity in Human Chromosomes and Interphase Nuclei of Normal., Leukemi C., and Tumor Cells as Evaluated by Silver Staining

Мамаев, Н Н; Mamaeva, Stella

doi:10.1016/s0074-7696(08)60661-0

Cited by 19 publications

(11 citation statements)

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“…It is likely that cells need rDNA expression, ribosome formation, and protein synthesis not only for proliferation but also for diVerentiation, maturation, repair, and so on. 12 Hence, the age related AgNOR positive proportion of cells may decrease in haemopoietic precursors to compensate/repair haemopoiesis in relation to insults to the haemopoietic system without a decrease in the proliferative activity of erythroid and granulocytic lineages. This would be in agreement with many clinical findings.…”

Section: Discussionmentioning

confidence: 99%

“…2 Studies on the AgNOR staining pattern in bone marrow cells from donors and patients with haematological malignancies have revealed significant decreases of modal AgNOR classes as well as a high percentage of AgNOR negative mitoses (mean, 48%; range, 34-70%) compared with PHA stimulated lymphocytes from the same subjects. [3][4][5][6][7][8][9][10][11][12][13] On the basis of these data, a hypothesis was put forward which related the AgNOR negative mitoses in bone marrows from healthy individuals and patients with leukaemias to polychromatic normoblasts and myelocytes, whose activity with regard to the synthesis of rRNA was clearly decreased. 4-6 12 14 This hypothesis has been verified by analysing the pattern of AgNOR staining in interphase nuclei of bone marrow erythroid and granulocytic elements of various maturities obtained from healthy donors and patients with leukaemias.…”

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confidence: 99%

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Age related decrease of NOR activity in bone marrow metaphase chromosomes from healthy individuals

Pedrazzini

Мамаев

Slavutsky

1998

Molecular Pathology

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Section: Discussionmentioning

confidence: 99%

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confidence: 99%

Age related decrease of NOR activity in bone marrow metaphase chromosomes from healthy individuals

Pedrazzini

Мамаев

Slavutsky

1998

Molecular Pathology

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“…Another important feature of change in the physiological status of a cell is the state of its nucleolar apparatus. There has been increasing interest in the nucleolus and its structure over the last 20 years (Jordan, 1987(Jordan, , 1991Mamaev and Mamaeva, 1990), for several reasons. Firstly, size, form and number of nucleoli are very important indices of the differentiation level and functional condition of a cell.…”

Section: Introductionmentioning

confidence: 99%

New indices in morphometry of nuclear structure in the NIH 3T3 cell line

Karalyan

2003

Cell Biology International

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We analyzed the connection of changes in nucleus ploidy with changes in nucleolar apparatus of NIH 3T3 cells. The quantity of nucleoli does not depend on the quantity of nucleolar DNA, but instead depends on euploidy: the majority of euploid cells have 1-3 nucleoli. The quantity of DNA in the nucleolus is correlated with the quantity of nucleolar DNA, and does not depend on ploidy changes. The nucleolar area has a tendency to increase in line with an increase in their numbers in the nucleus. The relationship of the quantity of DNA in the nucleolus with that of the nucleus is stable. During the process of increase in the number of nucleoli in a nucleus, there is a corresponding decrease in the quantity of DNA in each nucleolus, and there is likewise no increase in the sum of nucleolar DNA. The ratio of sums of the nucleolar perimeters to nuclear perimeter is a significant factor, which increases linearly along with an increase in the number of nucleoli in a nucleus.

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“…Li (Crocker 1990;Derenzini and Ploton 1991;Mamaev and Mamaeva 1990). Li (Crocker 1990;Derenzini and Ploton 1991;Mamaev and Mamaeva 1990).…”

Section: Introductionmentioning

confidence: 99%

Argyrophilic nucleolar organizer regions

Hacker

Danscher

et al. 1995

Histochem Cell Biol

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Silver staining techniques developed to demonstrate argyrophilic nucleolar organizer regions (Ag-NORs) have been widely applied in a variety of cell kinetic studies, using the mean number of AgNORs in tumour cells as a marker for malignancy of certain types of neoplasms. However, the AgNOR techniques currently available are not entirely satisfactory, as unspecific silver precipitates readily form in the sections. On the other hand, the contrast staining may be so weak as to render identification of the AgNORs difficult. In the present study, some of the key factors influencing the outcome of AgNOR staining were evaluated in a more systematic way. A modified AgNOR staining procedure is now proposed, giving highly contrasting AgNORs with minimal unspecific silver precipitation, thus facilitating both manual and computerized counting. The new technique involves the use of microwave irradiation in order to shorten the processing time, the use of gelatin as a protective colloid, and a Farmer's solution to optimize the specificity of the technique.

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Nucleolar Organizer Region Activity in Human Chromosomes and Interphase Nuclei of Normal., Leukemi C., and Tumor Cells as Evaluated by Silver Staining

Cited by 19 publications

References 145 publications

Age related decrease of NOR activity in bone marrow metaphase chromosomes from healthy individuals

Age related decrease of NOR activity in bone marrow metaphase chromosomes from healthy individuals

New indices in morphometry of nuclear structure in the NIH 3T3 cell line

Argyrophilic nucleolar organizer regions

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