NUCLEOLAR ORGANIZER REGIONS IN THE RABBIT (ORYCTOLAGUS CUNICULUS) AS SHOWN BY SILVER STAINING

Pa, Martin-DeLeon; Dl, Petrosky; Me, Fleming

doi:10.1139/g78-043

Cited by 11 publications

(3 citation statements)

References 3 publications

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“…For example, in the human these genes are found on five pairs of autosomes, in the mouse on eight pairs, in cattle also on five pairs, and in pig and rat on three chromosome pairs. Our data are in agreement with previous studies in the rabbit in which nucleolar organizer regions were identified at the same locations by sequential Qbanding and silver staining (Martin-DeLeon et al, 1978). Furthermore, the present data confirm the localization that was achieved by hybridization of RNA probes in radioactive in situ hybridization experiments (Martin-DeLeon, 1980).…”

Section: Resultssupporting

confidence: 93%

Fourteen chromosomal localizations and an update of the cytogenetic map of the rabbit

Zijlstra¹,

Haan²,

Korstanje

et al. 2002

Cytogenet Genome Res

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In order to improve the informativeness of the cytogenetic map of the rabbit genome, fourteen markers were regionally mapped to individual chromosomes. The localizations comprise eleven gene loci (PRLR, GHR, HK1, ACE, TF, 18S+28S rDNA, CYP2C4, PMP2, TCRB, ALOX15 and MT1) and three microsatellite loci (Sat13, Sol33 and D1Utr6). Five of the genes contain known microsatellite sequences. To achieve these localizations, homologous and heterologous small insert clones, and clones from a rabbit Bacterial Artificial Chromosome (BAC) library were used as probes for fluorescence in situ hybridization experiments. Results indicate that especially BAC clones are a valuable tool for cytogenetic mapping. Some of the genes were selected for mapping on the basis of human- rabbit comparative painting data, to achieve localizations on gene-poor rabbit chromosomes. Our data are, in general, in agreement with the human-rabbit comparative painting data. By mapping microsatellite sequences that have also been used in linkage studies, links are provided between the genetic and physical maps of the rabbit genome. Linkage groups I, VI and XI could be assigned to chromosomes 1, 5 and 3 respectively. Moreover, in this paper we give an overview of the current status of the rabbit cytogenetic map. This map now comprises 62 physically mapped genes, which are scattered over all autosomes, except chromosome 2, and the X chromosome.

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Section: Resultssupporting

confidence: 93%

Fourteen chromosomal localizations and an update of the cytogenetic map of the rabbit

Zijlstra¹,

Haan²,

Korstanje

et al. 2002

Cytogenet Genome Res

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“…The only possible comparison of Ag-NOR position is with Oryctolagus cuniculus (Martin-DeLeon 1980 ;Martin-DeLeon et al 1978 ;Monteagudo and Arruga 1991); this species shows the occurence of Ag-NORs in three pairs of chromosomes which might correspond to those of L. starcki, but also in a pair of small acrocentrics which do not find any homologue in our data.…”

Section: Resultsmentioning

confidence: 53%

Karyotypes of two endemic species of hare from Ethiopia, Lepus habessinicus and L. starcki (Lagomorpha, Leporidae). A comparison with L. europaeus

Puccetti¹,

Corti²,

Scanzani³

et al. 1996

Mammalia

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Karyotypes of two endemic species of hare from Ethiopia, Lepus habessinicus and L. starcki were studied and compared to that of L. europaeus. L. starcki has a fragmented range in the high Ethiopian plateaus and is considered a relict form of L. europaeus. In contrast, L. habessinicus occurs at lower altitudes, and is an arid-country species. The species share a 2n = 48 and a FNa = 88 common to all representatives of the genus. No sign of karyotypic differentiation is evident from G-banding and Ag-NOR location. These data support the hypothesis of pronounced karyotypic conservativism in the genus Lepus.Resume. -On a etudie le caryotype de deux especes de lievres endemiques d'Ethiopie, Lepus habessinicus et Lepus starcki et on les a compares ä celui de Lepus europaeus. L. starcki est distribue irregulierement sur le plateau ethiopien et est considere comme une forme relicte de L. europaeus, tandis que L. habessinicus vit ä des altitudes plus basses et est considere comme une espece xerophile. Les especes ethiopiennes partagent le meme caryotype ä 48 chromosomes (NFa = 88), commun a l'ensemble du genre Lepus. L'analyse des bandes G et Ag-NOR n'a mis en evidence aucune difference caryotypique entre les 3 especes. Ces donnoes confirment 1'hypothese que, chez les lievres, le caryotype est fortement conserve.

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“…The karyotype chromosomes are divided into three morphological groups: metacentric (34 autosomes), acrocentric (8 autosomes) and submetacentric (sex chromosomes) (ROBINSON et al 2002). The rabbit karyotype pattern of G, Q, R, C and NOR banded chromosomes has been determined (HAGELTRON & GUSTAVSSON 1979;POULSEN et al 1988;MARTIN-DELEON et al 1978;OEWITOÑSKI et al 1982;YERLE et al 1987;RqNNE 1995;KUCHTA et al 2009) and banding patterns have also been established. The rabbit karyotype has been used for comparisons with the human karyotype (DUTRILLAUX 1980).…”

mentioning

confidence: 99%

The Effect of Bromodeoxyuridine on Spontaneous Sister Chromatid Exchange Frequency in Rabbit (Oryctolagus cuniculus) and Coypu (Myocastor coypu) Chromosomes

Kuchta-Gładysz¹,

Wójcik²,

Szeleszczuk³

et al. 2016

folia biol (krakow)

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The effect of bromodeoxyuridine on spontaneous sister chromatid exchange frequency in rabbit (Oryctolagus cuniculus) and coypu (Myocastor coypu) chromosomes. Folia Biologica (Kraków) 64: 89-96. The sister chromatid exchange test is regarded as a highly sensitive cytogenetic assay. It measures chromosome sensitivity to particular damage factors and provides information on control and repair mechanism performance. It is instrumental in the early identification of the effects of noxious factors present in the habitat. This investigation was aimed at identifying sister chromatid exchange sites in coypu and rabbit chromosomes, as well as determining the spontaneity of the process by applying different BrdU doses. The chromosomes were obtained from an in vitro culture of blood lymphocytes, supplemented with 4 different BrdU doses: 0.25/0.5/1.0/2.5 µg/ml in order to identify spontaneous sister chromatid exchanges in both animal species. The chromosomes were stained according to the FPG method. Spontaneous SCEs were observed in coypu at a concentration of 1.0 µg/ml, and in rabbits at 0.5 µg/ml. The mean SCE/cell incidence was 1.41±1.15 in coypu, and 2.69±2.14 in rabbits. Differences in SCE incidence were identified between the analysed animal species and the applied BrdU doses.

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NUCLEOLAR ORGANIZER REGIONS IN THE RABBIT (ORYCTOLAGUS CUNICULUS) AS SHOWN BY SILVER STAINING

Cited by 11 publications

References 3 publications

Fourteen chromosomal localizations and an update of the cytogenetic map of the rabbit

Fourteen chromosomal localizations and an update of the cytogenetic map of the rabbit

Karyotypes of two endemic species of hare from Ethiopia, Lepus habessinicus and L. starcki (Lagomorpha, Leporidae). A comparison with L. europaeus

The Effect of Bromodeoxyuridine on Spontaneous Sister Chromatid Exchange Frequency in Rabbit (<I>Oryctolagus cuniculus</I>) and Coypu (<I>Myocastor coypu</I>) Chromosomes

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