Nucleolytic Activities of Mycoplasmas

Generation of site-specific mutations in Mycoplasma

“…General nucleases are indispensable in all organisms. In mycoplasmas they reside on the membrane providing important functions, presumably in the acquisition of nucleic acid precursors (31,32). Despite the challenges of working with an organism that grows poorly with a complex surface architecture, a major membrane-associated nuclease of Mycoplasma penetrans has been purified and studied (33).…”