2009
DOI: 10.1074/jbc.m109.005736
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Nucleophosmin Blocks Mitochondrial Localization of p53 and Apoptosis

Abstract: Activation of p53 is an important mechanism in apoptosis. However, whether the presence of p53 in mitochondria plays an important role in p53-mediated apoptosis is unclear. Here, we demonstrate that overexpression of NPM (

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Cited by 59 publications
(43 citation statements)
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“…Recent studies proved that BCLAF1 has a major role in T cell activation (87,88). Besides, nucleolar NPM1 suppresses apoptosis by inhibiting caspase-mediated activation of DNase, favoring genomic stability and DNA repair (89), as well as by blocking p53 mitochondrial localization (90). NPM1 is known to interact with HIV-1 Rev (91) and Tat, being critical for Tat nuclear localization and Tat-mediated transcription (92).…”
Section: Discussionmentioning
confidence: 99%
“…Recent studies proved that BCLAF1 has a major role in T cell activation (87,88). Besides, nucleolar NPM1 suppresses apoptosis by inhibiting caspase-mediated activation of DNase, favoring genomic stability and DNA repair (89), as well as by blocking p53 mitochondrial localization (90). NPM1 is known to interact with HIV-1 Rev (91) and Tat, being critical for Tat nuclear localization and Tat-mediated transcription (92).…”
Section: Discussionmentioning
confidence: 99%
“…NPM binds PKR and inhibits its pro-apoptotic activities both in mouse embryo fibroblasts (MEFs) and in Fanconi-anemia derived lymphoblasts (Pang et al, 2003). Dhar and St Clair (2009), recently showed that, although NPM overexpression favors stabilization of the tumor suppressor p53 and, consequently, results in increased levels of its targets p21 and BCL2-associated X protein (BAX), it simultaneously prevents the proapoptotic accumulation of p53 in the mitochondria, thus limiting the apoptotic response (Dhar and St Clair, 2009). On the other hand, it has been reported that, in neuronal cells, NPM, following apoptotic stimuli (such as staurosporine in vitro and ischemia in vivo), translocates from the nucleolus to the cytoplasm where it binds to BAX and p53 favoring mitochondrial cytochrome-c release and apoptosis (Kerr et al, 2007).…”
Section: Nucleophosmin (Npm) Is a Multi-functional Cellular Chaperonementioning
confidence: 99%
“…Then, the cells were washed in PBS three times and counterstained with DAPI. Fluorescence images were captured under a confocal microscope [15] .…”
Section: Cytotoxicity Assaymentioning
confidence: 99%