Nucleoplasm staining patterns and cell cycle–associated expression of Ki-67 in middle ear cholesteatoma

Marcadores de hiperproliferação na epiderme do meato acústico externo

“…Both in the EAC and in the tympanic membrane, the expression of this maker coincides with that from cytokeratin 16, showing the hyperproliferative characteristic of these regions. Schilling et al 9 , Adamczyk et al 10 , Raynov et al 11 and Hwang et al [1]12 observed a strong positiveness for Ki-67 on the EAC epidermis, without however specifying the location of where the samples were obtained from ( Figure 2). …”

“…As to study designs, 14 (87.5%) were cross-sectional [1][2][3][4][5][6][7][8][9][10][11][12][13][14] and only two (12.5%) were experimental 15,16 . Among all the papers included in this review, five (31.25%) were published with the only goal of analyzing, by means of immunohistochemistry, the epidermis of the normal external auditory meatus 1,4,5,8,16 .…”

“…Among all the papers included in this review, five (31.25%) were published with the only goal of analyzing, by means of immunohistochemistry, the epidermis of the normal external auditory meatus 1,4,5,8,16 . The other 11 studies (68.75%) were used only as control sample to assess the immunoexpression of cholesteatoma antigens, of the middle or external ear 2,7,8,[10][11][12][13][14][15][16] . Many markers were studied by means of the immunohistochemical methods in the investigations analyzed.…”

“…Nonetheless, with the progression of the studies and a greater understanding of the hyperproliferative characteristics of the EAC, many doubts will be increasingly near explanation such as, for example, the risk of developing middle ear cholesteatoma in patients with tympanic perforation, evaluation of the cholesteatoma aggressiveness or even the risk estimation of recurrence after surgical treatment. Another very much studied marker of hyperproliferation was the Ki-67nuclear antigen, which is a marker associated with cell proliferation and was studied in five papers [8][9][10][11][12] . All the authors found expressions of this marker in the normal external auditory canal; however, only one of the papers discusses where the samples were obtained from.…”

Edinilmiş kolesteatomda Langerhans hücrelerinin yeri: İleriye yönelik kontrollü kör klinik çalışma

“…7 Hücre ço¤almas› yönünde yap›lan çal›flmalarda Ki-67 ile immünohistokimyasal olarak iflaretlenmifl hücrelerin ço¤alma göstergesini (proliferation indeks) belirlemede en önde gelen yöntemlerden birisi oldu¤u tespit edilmifltir. 8,9 Apoptozis ile ilgili yap›lan çal›flmalarda kolesteatom patogenezinde önemli rol oynad›¤›, keratin debrislerin farkl›laflma ve birikiminin bir parças› oldu¤u bildirilmifltir. Kolesteatom epitelinde apoptozisin mekanizmas›n› ve apoptozis kapasitesini bilmek, bu s›k görülen kulak hastal›¤›n›n özgül davran›fllar›n› aç›klamada, hastal›¤›n sald›rganl›¤› hakk›n-da bilgi sahibi olmada ve tekrar etme e¤iliminin tespit etmede önemli bilgilere ›fl›k tutabilir.…”