2010
DOI: 10.1016/j.ejps.2010.09.013
|View full text |Cite
|
Sign up to set email alerts
|

Nucleoside transporter expression profiles in human cardiac tissue show striking individual variability with overall predominance of hENT1

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
2
1
1
1

Citation Types

1
4
0

Year Published

2011
2011
2021
2021

Publication Types

Select...
5
1

Relationship

2
4

Authors

Journals

citations
Cited by 6 publications
(5 citation statements)
references
References 39 publications
1
4
0
Order By: Relevance
“…These data suggest that a similar mechanism of ethanol-mediated inhibition of ENT1 exists in multiple cell types. Ethanol-specific inhibition of ENT1 uptake (rather than ENT2) in cardiomyocytes is also consistent with previous studies showing that ENT2, which is expressed in much lower amounts than ENT1 in HL-1 cells [5], mouse, and human heart tissue [3,30], is insensitive to ethanol [8]. Ethanol is thought to interact with ENT1 at a specific site in the protein as has been described for other ethanol-sensitive membrane proteins such as the N-methyl-D-aspartate receptors, gammaamino butyric acid receptors, serotonin receptors, calciumand voltage-activated potassium channels (BK Ca ), and ligand-gated ion channels [22,31,32].…”
Section: Discussionsupporting
confidence: 76%
“…These data suggest that a similar mechanism of ethanol-mediated inhibition of ENT1 exists in multiple cell types. Ethanol-specific inhibition of ENT1 uptake (rather than ENT2) in cardiomyocytes is also consistent with previous studies showing that ENT2, which is expressed in much lower amounts than ENT1 in HL-1 cells [5], mouse, and human heart tissue [3,30], is insensitive to ethanol [8]. Ethanol is thought to interact with ENT1 at a specific site in the protein as has been described for other ethanol-sensitive membrane proteins such as the N-methyl-D-aspartate receptors, gammaamino butyric acid receptors, serotonin receptors, calciumand voltage-activated potassium channels (BK Ca ), and ligand-gated ion channels [22,31,32].…”
Section: Discussionsupporting
confidence: 76%
“…ENT2 has a higher affinity for inosine than ENT1 (Belt and Noel, 1985) but we show here it can transport adenosine in the absence of ENT1 although it has been reported to have a lower affinity for adenosine as a substrate (Kiss et al, 2000). While ENT2 is present at much lower levels than ENT1 in mouse and human heart tissue Marvi et al, 2010), these data demonstrate that this isoform, particularly in the absence of ENT1, has the capacity to influence purine nucleoside levels in cardiomyocytes and its contribution to purinergic signaling in the cardiovasculature should be taken into account when considering the role of nucleoside uptake in purinergic responses. Inosine and adenosine can be formed from IMP hydrolysis (Willems et al, 2006) and IMP levels are significantly elevated in ENT1-null cardiomyocytes in response to hypoxic challenge suggesting that the presence and activity of ENT1 and ENT2 can have effects on extracellular pools of nucleotides far upstream of its immediate substrate.…”
Section: Discussionmentioning
confidence: 61%
“…Nucleoside analogs are routinely used in conjunction with platinum-based chemotherapeutics, such as cisplatin, and have been shown to have an enhanced effect when compared to individual treatments for a wide range of cancers (e.g., pancreatic, breast, non-small-cell lung cancers) (166169). ENT inhibitors are also used to treat epilepsy and various cardiac conditions that require use of antiplatelet agents, calcium channel blockers, and vasodilators (31, 32, 81, 104). …”
Section: Physiological Roles Of Ent Functionmentioning
confidence: 99%