Nucleosphaerium tuckeri nov. gen. nov. sp. — A new freshwater filose amoeba without motile form in a new family nucleariidae (Filosea: Aconchulinida) feeding by ingestion only

Cann, J.P.; Page, F. Zeb

doi:10.1016/s0003-9365(79)80034-2

Cited by 19 publications

(19 citation statements)

References 12 publications

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“…The amoeba belongs to the genus Nuclearia, the type genus of the family Nucleariidae, which was originally assigned to the class Filosea by Cann and Page (1979). Based on molecular phylogenetic analyses, nucleariid amoebae are a sister group of Fungi (Brown et al 2009) belonging to the diverse group of Opisthokonta (Liu et al 2009;Steenkamp et al 2006;Zettler et al 2001).…”

Section: Introductionmentioning

confidence: 99%

“…One typical feature of some Nuclearia species is the excretion of extracellular polymeric substances (EPS) that form a mucous sheath around the cell -already observed by Artari (1889). A positive periodic acid-schiff (PAS) staining of the EPS, indicating its polysaccharide character, was shown for N. radians (described as Nucleosphaerium tuckeri by Cann and Page (1979)), for N. delicatula (Cann 1986) and for N. simplex (Pernin 1976). The EPS can be colonized by ectosymbiotic bacteria that are arranged regularly a few micrometres from the cell membrane (Cann 1986;Cann and Page 1979;Patterson 1984).…”

Section: Introductionmentioning

confidence: 99%

“…So far, endosymbiotic bacteria have been detected in two nucleariid species. N. radians harbours endosymbionts that are surrounded by a peribacterial membrane in the cytoplasm (Cann and Page 1979), and the amphizoic N. pattersoni has a rickettsial endosymbiont (Dyková et al 2003).…”

Section: Introductionmentioning

confidence: 99%

“…Besides being already a complex community of three microorganisms, several nucleariid amoebae have a peculiar feeding behaviour with respect to prey ingestion and selection (Artari 1889;Cann 1986;Cann and Page 1979). These Nuclearia species can ingest toxic filamentous cyanobacteria as their sole source of food.…”

Section: Introductionmentioning

confidence: 99%

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Ménage-à-trois: The Amoeba Nuclearia sp. from Lake Zurich with its Ecto- and Endosymbiotic Bacteria

Dirren

Salcher

Blom

et al. 2014

Protist

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We present a fascinating triad relationship between a eukaryotic amoeba and its two bacterial symbionts. The morphological characteristics of the amoeba allowed for a confident assignment to the genus Nuclearia (Opisthokonta, Nucleariidae), but species identification resulted in an ambiguous result. Sequence analysis indicated an affiliation to the species N. thermophila, however, several morphological features contradict the original description. Amoebal isolates were cultured for several years with their preferred food source, the microcystin-producing harmful cyanobacterium Planktothrix rubescens. Symbioses of the amoeba with ecto-and endosymbiotic bacteria were maintained over this period. Several thousand cells of the ectosymbiont are regularly arranged inside a layer of extracellular polymeric substances produced by the amoeba. The ectosymbiont was identified as Paucibacter toxinivorans (Betaproteobacteria), which was originally isolated by enrichment with microcystins. We found indications that our isolated ectosymbiont indeed contributed to toxin-degradation. The endosymbiont (Gammaproteobacteria, 15-20 bacteria per amoeba) is enclosed in symbiosomes inside the host cytoplasm and represents probably an obligate symbiont. We propose the name "Candidatus Endonucleariobacter rarus" for this bacterium that was neither found free-living nor in a symbiotic association. Nucleariidae are uniquely suited model organisms to study the basic principles of symbioses between opisthokonts and prokaryotes. 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 We present a fascinating triad relationship between a eukaryotic amoeba and its two bacterial

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Ménage-à-trois: The Amoeba Nuclearia sp. from Lake Zurich with its Ecto- and Endosymbiotic Bacteria

Dirren

Salcher

Blom

et al. 2014

Protist

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“…Nucleosphaerium which has a spherical body with radiating filose pseudopodia is also similar to the Nuclearia sp. The organism described by CANN and PAGE (15) has an enveloping mucus sheath, composed of three layers seen after the cells are fixed in 200 osmium tetroxide. No such mucilaginous coat was observed in the Nuclearia sp.…”

Section: Discussionmentioning

confidence: 99%

Light and electron microscope observations and prey specificities of an algophorous amoeba from Japanese freshwater.

Yamamoto

Suzuki

1984

J. Gen. Appl. Microbiol.

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Observations on “Vampyrella Penula‐stylodinium Sphaera” and the Ultrastructure of the Reproductive Cyst

Timpano

Pfiester

1986

American J of Botany

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“Vampyrella‐Stylodinium,” an artificial name for a predaceous organism of uncertain taxonomic position, has at least three distinct phases in its life history: the amoeboid phase, both free‐floating and attached; the feeding cyst or immobile phase; and flagellated gymnodinoid swarmers. The orange free‐floating amoeba has unbranched, filose pseudopodia and several contractile vacuoles. When feeding on the filamentous green alga Oedogonium, the pseudopodia shorten and rearrange. After dissolution of part of the Oedogonium cell wall, the amoeba ingests the host protoplast. Then a stalked reproductive cyst may form. This cyst changes color from green to light orange as it matures. At the time of excystment, the cyst has a smooth outer wall, a spinose inner wall, and a well‐delineated phagocytic vacuole. As this vacuole moves from its central position to the cyst's periphery, the walls rupture and 2‐4 amoebulae emerge. With TEM observations, the reproductive cyst is shown to be multinucleate. Each nucleus is eukaryotic in organization and possesses one nucleolus. Mitochondria have tubular cristae and no structures unique to the division Pyrrhophyta are observed. Although this stage of the life history does not have a dinokaryotic nucleus, the gymnodinoid swarmers that can emerge from the reproductive cyst, do. Like other parasites which have been assigned to the division Pyrrhophyta, “Vampyrella‐Stylodinium” does not conform well to the generalized concept of a dinoflagellate.

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Nucleosphaerium tuckeri nov. gen. nov. sp. — A new freshwater filose amoeba without motile form in a new family nucleariidae (Filosea: Aconchulinida) feeding by ingestion only

Cited by 19 publications

References 12 publications

Ménage-à-trois: The Amoeba Nuclearia sp. from Lake Zurich with its Ecto- and Endosymbiotic Bacteria

Ménage-à-trois: The Amoeba Nuclearia sp. from Lake Zurich with its Ecto- and Endosymbiotic Bacteria

Light and electron microscope observations and prey specificities of an algophorous amoeba from Japanese freshwater.

Observations on “Vampyrella Penula‐stylodinium Sphaera” and the Ultrastructure of the Reproductive Cyst

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