Nucleotidase and DNase activities in Brazilian snake venoms

Sales, Paulo Bruno Valadão; Santoro, Marcelo Larami

doi:10.1016/j.cbpc.2007.08.003

Cited by 40 publications

(26 citation statements)

References 52 publications

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“…Liberated orthophosphate was estimated by adding 600 ml of the colouring agent (81.1 mM ascorbic acid, 3 mM ammonium molybdate and 0.5 M H 2 SO 4 ) and 300 ml of deionised water (Sales and Santoro, 2008). Absorbance at 820 nm was monitored to estimate orthophosphate.…”

Section: Enzyme Assaysmentioning

confidence: 99%

“…All data points were obtained from three independent experiments. 5 0 -nucleotidase, ADPase and ATPase activities were determined from liberation of orthophosphate from the substrates AMP, ADP and ATP respectively (Sales and Santoro, 2008). Briefly, 50 ml of the enzyme solution was incubated with 50 ml of 1 mM of the substrates as applicable in 100 mM glycine-NaOH, pH 8.9 at 37 C for 30 min.…”

Section: Enzyme Assaysmentioning

confidence: 99%

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Phosphodiesterase from Daboia russelli russelli venom: Purification, partial characterization and inhibition of platelet aggregation

Mitra

Bhattacharyya

2014

Toxicon

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Section: Enzyme Assaysmentioning

confidence: 99%

Section: Enzyme Assaysmentioning

confidence: 99%

Phosphodiesterase from Daboia russelli russelli venom: Purification, partial characterization and inhibition of platelet aggregation

Mitra

Bhattacharyya

2014

Toxicon

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“…The venom of Loxosceles spiders is a complex mixture of protein and peptide toxins with a molecular mass profile ranging from 1 to 40 kDa [5]. To date, several molecules in the Loxosceles spider crude venoms have been described, including alkaline phosphatase [5,10], 5’-ribonucleotide phosphohydrolase [5], sulfated nucleosides [11], hyaluronidase [5,12,13,14], fosfolipases-D [5,15,16,17], metalloproteases, serine proteases [12,13,18,19,20,21,22] and insecticide toxins [23]. Table 1 contains a brief collection of main features from proteic toxins described in Loxosceles genus.…”

Section: The Loxosceles Venomsmentioning

confidence: 99%

Brown Spider (Loxosceles genus) Venom Toxins: Tools for Biological Purposes

Chaim

Trevisan-Silva

Chaves-Moreira

et al. 2011

Toxins

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Venomous animals use their venoms as tools for defense or predation. These venoms are complex mixtures, mainly enriched of proteic toxins or peptides with several, and different, biological activities. In general, spider venom is rich in biologically active molecules that are useful in experimental protocols for pharmacology, biochemistry, cell biology and immunology, as well as putative tools for biotechnology and industries. Spider venoms have recently garnered much attention from several research groups worldwide. Brown spider (Loxosceles genus) venom is enriched in low molecular mass proteins (5–40 kDa). Although their venom is produced in minute volumes (a few microliters), and contain only tens of micrograms of protein, the use of techniques based on molecular biology and proteomic analysis has afforded rational projects in the area and permitted the discovery and identification of a great number of novel toxins. The brown spider phospholipase-D family is undoubtedly the most investigated and characterized, although other important toxins, such as low molecular mass insecticidal peptides, metalloproteases and hyaluronidases have also been identified and featured in literature. The molecular pathways of the action of these toxins have been reported and brought new insights in the field of biotechnology. Herein, we shall see how recent reports describing discoveries in the area of brown spider venom have expanded biotechnological uses of molecules identified in these venoms, with special emphasis on the construction of a cDNA library for venom glands, transcriptome analysis, proteomic projects, recombinant expression of different proteic toxins, and finally structural descriptions based on crystallography of toxins.

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“…It is related to blood‐feeding because of apyrase's ability to hydrolyse adenosine diphosphate (ADP), a key component of platelet aggregation. Recently, ADPases were also found in Brazilian snake venoms (Sales & Santoro, 2008).…”

mentioning

confidence: 99%

Insights into the venom composition of the ectoparasitoid wasp Nasonia vitripennis from bioinformatic and proteomic studies

Graaf

Aerts

Brunain

et al. 2010

Insect Molecular Biology

179

237

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With the Nasonia vitripennis genome sequences available, we attempted to determine the proteins present in venom by two different approaches. First, we searched for the transcripts of venom proteins by a bioinformatic approach using amino acid sequences of known hymenopteran venom proteins. Second, we performed proteomic analyses of crude N. vitripennis venom removed from the venom reservoir, implementing both an off-line two-dimensional liquid chromatography matrix-assisted laser desorption/ionization time-of-flight (2D-LC-MALDI-TOF) mass spectrometry (MS) and a two-dimensional liquid chromatography electrospray ionization Founer transform ion cyclotron resonance (2D-LC-ESI-FT-ICR) MS setup. This combination of bioinformatic and proteomic studies resulted in an extraordinary richness of identified venom constituents. Moreover, half of the 79 identified proteins were not yet associated with insect venoms: 16 proteins showed similarity only to known proteins from other tissues or secretions, and an additional 23 did not show similarity to any known protein. Serine proteases and their inhibitors were the most represented. Fifteen nonsecretory proteins were also identified by proteomic means and probably represent so-called ‘venom trace elements’. The present study contributes greatly to the understanding of the biological diversity of the venom of parasitoid wasps at the molecular level.

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Nucleotidase and DNase activities in Brazilian snake venoms

Cited by 40 publications

References 52 publications

Phosphodiesterase from Daboia russelli russelli venom: Purification, partial characterization and inhibition of platelet aggregation

Phosphodiesterase from Daboia russelli russelli venom: Purification, partial characterization and inhibition of platelet aggregation

Brown Spider (Loxosceles genus) Venom Toxins: Tools for Biological Purposes

Insights into the venom composition of the ectoparasitoid wasp Nasonia vitripennis from bioinformatic and proteomic studies

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