Nucleotide and deduced amino acid sequence of stp: The bacteriophage T4 anticodon nuclease gene

Chapman, Daphne; Morad, Ilan; Kaufmann, Gabriel; Gait, Michael J.; Jorissen, Lori; Snyder, Larry

doi:10.1016/0022-2836(88)90320-8

Cited by 18 publications

(7 citation statements)

References 23 publications

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“…Rtp6 is a short protein of 30 aa that is highly similar to the 27-residue Stp protein of T4 (3,22,57) and related phages (79% identity over 24 residues). Rtp6 is the first Stp homolog outside the T4-like phages.…”

Section: Vol 188 2006mentioning

confidence: 99%

The Genome of the Novel Phage Rtp, with a Rosette-Like Tail Tip, IsHomologous to the Genome of Phage T1

et al. 2006

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A new Escherichia coli phage, named Rtp, was isolated and shown to be closely related to phage T1. Electron microscopy revealed that phage Rtp has a morphologically unique tail tip consisting of four leaf-like structures arranged in a rosette, whereas phage T1 has thinner, flexible leaves that thicken toward the ends. In contrast to T1, Rtp did not require FhuA and TonB for infection. The 46.2-kb genome of phage Rtp encodes 75 open reading frames, 47 of which are homologous to phage T1 genes. Like phage T1, phage Rtp encodes a large number of small genes at the genome termini that exhibit no sequence similarity to known genes. Six predicted genes larger than 300 nucleotides in the highly homologous region of Rtp are not found in T1. Two predicted HNH endonucleases are encoded at positions different from those in phage T1. The sequence similarity of rtp37, -38, -39, -41, -42, and -43 to equally arranged genes of lambdoid phages suggests a common tail assembly initiation complex. Protein Rtp43 is homologous to the J protein, which determines host specificity. Since the two proteins differ most in the C-proximal area, where the binding site to the LamB receptor resides in the J protein, we propose that Rtp43 contributes to Rtp host specificity. Lipoproteins similar to the predicted lipoprotein Rtp45 are found in a number of phages (encoded by cor genes) in which they prevent superinfection by inactivating the receptors. We propose that, similar to the proposed function of the phage T5 lipoprotein, Rtp45 prevents inactivation of Rtp by adsorption to its receptor during cells lysis. Rtp52 is a putative transcriptional regulator, for which 10 conserved inverted repeats were identified upstream of genes in the Rtp genome. In contrast, the much larger E. coli genome has only one such repeat sequence.Phages form the largest group of "organisms" on earth and display a huge genetic diversity. To date, 228 phage genomes have been sequenced and the 252 sequenced bacterial genomes contain many prophages and phage-like elements. Horizontal gene transfer between phages and their hosts largely contributes to the genetic variety of the phages and their hosts. During evolution, phages endowed bacteria with new functions enabling them to occupy new environmental niches. Phage genomics provides data for tracing phage and bacterial evolution. It is therefore desirable to determine the sequences of as many phages as possible.Escherichia coli and a selected group of E. coli phages paved the way into molecular biology. Among the phages was T1 (1, 29), whose genome sequence was, however, not determined until 2004, over 60 years after its discovery (75). The phage T1 genome encodes 77 open reading frames (ORFs), 37 of which are homologous to described phage ORFs. Functions have been assigned to 27 ORFs. The largest functional group consists of tail proteins. Twenty-two ORFs encoding putative proteins of fewer than 100 amino acid (aa) residues lie predominantly close to the termini. These putative proteins display no sequence similarity to...

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Section: Vol 188 2006mentioning

confidence: 99%

The Genome of the Novel Phage Rtp, with a Rosette-Like Tail Tip, IsHomologous to the Genome of Phage T1

et al. 2006

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“…Pnk and Rnl1 are dispensable for T4 growth on common E. coli laboratory strains but required on a rare host encoding the optional locus prr (pnk and rnl1 restriction) (Depew & Cozzarelli, 1974;Sirotkin et al, 1978;Runnels et al, 1982;Jabbar & Snyder, 1984). Mutating a minuscule T4 orf termed stp (suppressor of three-prime phosphatase) abrogates prr restriction (Depew & Cozzarelli, 1974;Depew et al, 1975;Chapman et al, 1988;Penner et al, 1995). These facts reinforced the notion that Pnk and Rnl1 cooperate in RNA nick repair.…”

Section: Prrc -A Potential Phage-excluding Tool Counteracted By Trna mentioning

confidence: 93%

RloC: A Translation-Disabling tRNase Implicated in Phage Exclusion During Recovery from DNA Damage

Kaufmann¹,

Davidov²,

Steinfels-Kohn³

et al. 2011

DNA Repair

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“…The inhibitory components constitute a type IC DNA restriction-modification enzyme termed Ecoprrl (4-6). The small T4-encoded polypeptide Stp alleviates Ecoprrl restriction (7) and also activates ACNase (8,9). The study of ACNase may provide insights into the evolution ofmechanisms ofphage exclusion and protein-catalyzed RNA splicing.…”

Section: Introductionmentioning

confidence: 99%

Cleavage of the HIV replication primer tRNALys,3 in human cells expressing bacterial anticodon nuclease

Shterman

Elroy‐Stein

Morad

et al. 1995

Nucleic Acids Research

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Nucleotide and deduced amino acid sequence of stp: The bacteriophage T4 anticodon nuclease gene

Cited by 18 publications

References 23 publications

The Genome of the Novel Phage Rtp, with a Rosette-Like Tail Tip, IsHomologous to the Genome of Phage T1

The Genome of the Novel Phage Rtp, with a Rosette-Like Tail Tip, IsHomologous to the Genome of Phage T1

RloC: A Translation-Disabling tRNase Implicated in Phage Exclusion During Recovery from DNA Damage

Cleavage of the HIV replication primer tRNALys,3 in human cells expressing bacterial anticodon nuclease

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