Nucleotide sequence analysis of a chloramphenicol-resistance determinant from Agrobacterium tumefaciens and identification of its gene product

Tennigkeit, J.; Matzura, Hans

doi:10.1016/0378-1119(91)90112-o

Cited by 49 publications

(47 citation statements)

References 15 publications

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“…The cat gene itself has no homology to other cat genes, with the exception of the recently described chromosomal cat gene of A. tumefaciens (45). Use (46).…”

Section: Discussionmentioning

confidence: 99%

“…Downie (14) 5. Amino acid similarities between the plasmid-specified CAT protein of Tn2424 and chromosomally specified CAT proteins from P. aeruginosa (18,40) and A. tumefaciens (45) and partial sequences of presumptive CAT proteins from B. sphaericus (30) and S. aureus (1). The last two sequences are unidentified reading frames adjacent to other antibiotic resistance genes which were the objects of the publications cited.…”

Section: Agaccttcgtcctgagatccgagctcctagtcgcgagttgcagcgacggcatcgtcggctmentioning

confidence: 99%

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The chloramphenicol acetyltransferase gene of Tn2424: a new breed of cat

Parent

Roy

1992

J Bacteriol

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We have sequenced the gene coding for the chloramphenicol acetyltransferase of Tn2424 of plasmid NR79. This gene codes for a protein of 23,500 Da, and the derived protein sequence is similar to those of the chromosomal chloramphenicol acetyltransferases of Agrobacterium tumefaciens and Pseudomonas aeruginosa and of unidentified open reading frames, which may encode chloramphenicol acetyltransferases, adjacent to the ermG macrolide-lincosamide-streptogramin resistance gene of Bacillus sphaericus and the vgb virginiamycin resistance gene of Staphylococcus aureus. Weaker similarity to the LacA (thiogalactoside acetyltransferase) and CysE (serine acetyltransferase) proteins of Escherichia coli and the NodL protein of Rhizobium leguminosarum is also observed. There is no significant similarity to any other chloramphenicol acetyltransferase genes, such as that of Tn9. The Tn2424 cat gene is part of a 4.5-kb region which also contains the aacA1a aminoglycoside-6'-N-acetyltransferase gene; Tn2424 is similar to Tn21 except for the presence of this region. Sequences flanking the cat gene are typical of those flanking other genes inserted into pVS1-derived "integrons" by a site-specific recombinational mechanism.

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“…The cat gene itself has no homology to other cat genes, with the exception of the recently described chromosomal cat gene of A. tumefaciens (45). Use (46).…”

Section: Discussionmentioning

confidence: 99%

Section: Agaccttcgtcctgagatccgagctcctagtcgcgagttgcagcgacggcatcgtcggctmentioning

confidence: 99%

The chloramphenicol acetyltransferase gene of Tn2424: a new breed of cat

Parent

Roy

1992

J Bacteriol

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“…These proteins include, among others, WbeO from V. cholerae (accession S28479 ; Stroeher et al, 1995), WbbJ from E. coli K-12 (accession P37750 ; Yao & Valvano, 1994), CapG from Staphylococcus aureus (accession P39856 ; Lin et al, 1994), NodL from Rhizobium (Sinorhizobium) meliloti (accession P28266 ; Baev & Kondorosi, 1992) and Cat4 from Agrobacterium tumefaciens (accession P23364 ; Tennigkeit & Matzura, 1991). The proteins from this family are involved in the transfer of acetyl groups from acetylCoA and share a region of homology along the Cterminal 50-70 amino acids (Fig.…”

Section: Figmentioning

confidence: 99%

Genetic organization of the O7-specific lipopolysaccharide biosynthesis cluster of Escherichia coli VW187 (O7:K1) The GenBank accession number for the DNA sequence reported in this paper is AF125322.

1999

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In previous studies the authors cloned and characterized the DNA sequence of the regions at both ends of the O7-specific lipopolysaccharide (LPS) biosynthesis cluster of Escherichia coli VW187 (O7 :K1), and identified the biosynthetic genes for dTDP-rhamnose and GDP-mannose, as well as one of the candidate glycosyltransferases. In this work the complete DNA sequence of a 69 kb intervening region is presented. Seven new ORFs were identified. All the functions required for the synthesis and transfer of the O7 LPS were assigned on the basis of complementation experiments of transposon insertion mutants, and amino acid sequence homology to proteins involved in LPS synthesis of other bacteria. Of the seven ORFs, two encoded membrane proteins that were homologous to the O-antigen translocase (Wzx) and polymerase (Wxy), two were involved in the biosynthesis of dTDP-Nacetylviosamine, and the remaining three showed homologies to sugar transferases. The O antigen chain length regulator gene wzz was also identified in the vicinity of the O7 polysaccharide cluster. O7-specific DNA primers were designed and tested for serotyping of O7 E. coli strains.

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“…Similar recombinations were found to occur in pGSA1252 constructs containing shorter sense and antisense sequences (150 bp long; data not shown). pGSA1252 has a chloramphenicol-resistant selection marker, and many Agrobacterium strains such as C58 (Huang et al, 2006), GV3101 (Pitino et al, 2011;Zhang et al, 2011), and A136 and BG53 (Rogers et al, 2002) that are routinely used for A. thaliana transformation have been reported to possess an inducible resistance mechanism against this antibiotic (Tennigkeit and Matzura, 1991), suggesting that chloramphenicol selection causes recombination with many Agrobacterium strains (Rogers et al, 2002;Bent, 2006;Szakasits et al, 2007). Therefore, we decided to use a vector with a kanamycin-selectable marker, pMDC32, since kanamycin is an appropriate antibiotic for selection of Agrobacterium (Bent, 2006;Szakasits et al, 2007).…”

Section: Development Of Transgenic Arabidopsis Plants and Verificatiomentioning

confidence: 99%

“…The procedure is relatively complex and time consuming, and problems with plasmid instability related to antibiotic selection have been reported. For example, many Agrobacterium strains, such as C58 (Huang et al, 2006), GV3101 (Pitino et al, 2011;Zhang et al, 2011), and A136 and BG53 (Rogers et al, 2002) that are routinely used for Arabidopsis thaliana transformation possess an inducible resistance mechanism against chloramphenicol (Tennigkeit and Matzura, 1991), suggesting that effective selection with this antibiotic is not possible with many Agrobacterium strains (Rogers et al, 2002;Bent, 2006;Szakasits et al, 2007). This response also leads to promiscuous recombination and plasmid instability (Ballester et al, 1986(Ballester et al, , 1989.…”

Section: Introductionmentioning

confidence: 99%

Development of an improved RNA interference vector system for Agrobacterium-mediated plant transformation

Toprak

Coutu²,

Baldwin³

et al. 2014

Turk J Biol

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Plant-mediated RNA interference (RNAi) has shown a great potential in pest control and requires i) subcloning of sense/ antisense regions in compatible vectors, ii) transfer of the silencing cassette into a binary vector, iii) transformation of Agrobacterium tumefaciens with desired binary plasmids, and iv) transformation of plants with Agrobacterium. The procedure is long and should ensure plasmid backbone stability; however, plasmid recombination due to antibiotic selection is a common problem. pGSA1252 is an RNAi silencing binary vector allowing direct cloning of hairpin structure; however, it possesses a chloramphenicol selection marker leading to plasmid recombination in various Agrobacterium strains. To solve this selection marker/Agrobacterium compatibility problem and to shorten the cloning process, we developed a new RNAi vector system containing the RNAi cassette of pGSA1252 in a plant expression vector, pMDC32, which has kanamycin as the selection marker. A T7 RNA polymerase promoter was also incorporated adjacent to the multiple cloning site, allowing for in vitro dsRNA synthesis. This vector was tested by transforming Arabidopsis thaliana with 4 different dsRNA constructs specific to insect midgut genes: insect intestinal mucin 1/4, peritrophic matrix protein 1, chitin deacetylase 1, and chitin synthase-B. This improved system shows no recombination and shortens the entire cloning procedure.

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Nucleotide sequence analysis of a chloramphenicol-resistance determinant from Agrobacterium tumefaciens and identification of its gene product

Cited by 49 publications

References 15 publications

The chloramphenicol acetyltransferase gene of Tn2424: a new breed of cat

The chloramphenicol acetyltransferase gene of Tn2424: a new breed of cat

Genetic organization of the O7-specific lipopolysaccharide biosynthesis cluster of Escherichia coli VW187 (O7:K1) The GenBank accession number for the DNA sequence reported in this paper is AF125322.

Development of an improved RNA interference vector system for Agrobacterium-mediated plant transformation

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