Nucleotide sequence analysis of DNA

Wü, Ray; Taylor, Ellen

doi:10.1016/0022-2836(71)90105-7

Cited by 241 publications

(37 citation statements)

References 12 publications

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“…Nearest neighbour analysis of the oligonucleotides was carried out by combined digestion with micrococcal nuclease and spleen phosphodiesterase (Worthington Biochemicals Corp., Freehold, New Jersey, USA [4].…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Nucleotide sequence adjacent to polyadenylic acid in globin messenger RNA

Proudfoot¹,

Brownlee²

1974

FEBS Letters

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Section: Methodsmentioning

confidence: 99%

“…This was achieved by omitting one or more of the deoxyribonucleoside triphosphates from the reaction, thus restricting the elongation of the primer to a few bases [4]. The initial approach was to incubate mRNA in the presence of one labelled triphosphate.…”

Section: Sequence Studiesmentioning

confidence: 99%

Nucleotide sequence adjacent to polyadenylic acid in globin messenger RNA

Proudfoot¹,

Brownlee²

1974

FEBS Letters

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“…An obvious enzyme to choose for copying DNA was DNA polymerase, which had already been used by Wu & Kaiser in 1969 (44) to determine the sequence of the "sticky ends" of bacteriophage A. This dodecanucleotide sequence was in fact the first bit of DNA to be sequenced (45). An elegant approach to specific digestion of DNA, which could be com bined with a copying procedure , was suggested by Berg , Fancher, & Cham berlin in 1963 (46).…”

Section: Dnamentioning

confidence: 99%

Sequences, Sequences, and Sequences

Sanger

1988

Annu. Rev. Biochem.

188

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“…Elegant work on determination of the sequence of the 5' single-stranded ends has been done by Wu et al. (6,7) and by Murray and Murray (2). Information on the 3'-termini sequences comes primarily from the work of Weigel et al (8) and our own effort (ref.…”

mentioning

confidence: 99%

Alignment of Two DNA Helices: A Model for Recognition of DNA Base Sequences by the Termini-Generating Enzymes of Phage λ, 186, and P2

Wang

Brezinski

1973

Proc. Natl. Acad. Sci. U.S.A.

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Based on the 3'-and 5'-terminal sequences of DNA of phage X, P2, and 186, a model is proposed for recognition of DNA sequences by enzymes responsible for generation of cohesive ends. Two copies of the cohered ends, either on separate molecules or on a concatemer, are aligned with their helical axes parallel but running in opposite directions. The nicking system is dimeric, with each of the two monomers carrying identical sequencerecognition sites. Two pairs of nicks are introduced into the two aligned DNA molecules by the nicking system. The applicability of this model to other biological processes, such as integration of a viral genome into a host genome and the cutting of concatemeric T7 DNA, is discussed.For several phages, the DNA extracted from mature phage has short, single-stranded ends with complementary base sequences (1). For coliphages, it appears that there are only two classes of cohesive ends, with those of the lambdoid phages (X, 480, 21, 82, 424, and 434) in one class and those of the 186 family (including 186, P2, P4, and 299) in another (1, 2). Within each class base sequences of the ends are identical or very similar. The base sequences of X-type cohesive ends and 186-type cohesive ends are different, and mutual joining does not occur. Shortly after infection, the cohesive ends join and the two single-chain interruptions in the resulting molecule are subsequently sealed by ligase (1). Therefore, in order to regenerate the single-stranded ends before maturation of the phage, whether from a concatemer or from a circular DNA, two staggered single-chain scissions must be introduced at unique points of the DNA molecule. The enzyme or enzymes involved in this process has been named the termini-generating enzyme or Ter for short (3, 4). For X, it appears that the phage gene-A product provides the Ter function, although participation of host functions and X genes between R and A has not been ruled out (5).We studied the problem of DNA sequence recognition involved in the Ter system, aside from interests in this system itself, because the DNA sequences flanking the sites of endonucleolytic nicking are present at the 3' and 5' termini of mature phage DNA; therefore, the base sequences can be determined without the formidable difficulties of determining the sequence of a segment in the middle of a DNA molecule. Elegant work on determination of the sequence of the 5' single-stranded ends has been done by Wu et al. (6,7) and by Murray and Murray (2). Information on the 3'-termini sequences comes primarily from the work of Weigel et al. (8) and our own effort (ref. 9; D. P. Brezinski and J. C. Wang, unpublished). 2667 In this communication, we postulate a model for recognition of DNA base sequences by Ter, based on sequence information. We believe that sequence recognition in the Ter system involves alignment of two copies of the cohered ends with their helical axes parallel but running in opposite directions. We further postulate that the alignment is achieved by a sequence-specific protein s...

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Nucleotide sequence analysis of DNA

Cited by 241 publications

References 12 publications

Nucleotide sequence adjacent to polyadenylic acid in globin messenger RNA

Nucleotide sequence adjacent to polyadenylic acid in globin messenger RNA

Sequences, Sequences, and Sequences

Alignment of Two DNA Helices: A Model for Recognition of DNA Base Sequences by the Termini-Generating Enzymes of Phage λ, 186, and P2

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