Nucleotide sequence analysis of two nuclear inclusion body and coat protein genes of a sweet potato feathery mottle virus severe strain (SPFMV-S) genomic RNA

Mori, Masaki; Sakai, Jun‐ichi; Kimura, Takashi; Usugi, Tomio; Hayashi, Toshimitsu; Hanada, Kaoru; Nishiguchi, Masamichi

doi:10.1007/bf01322674

Cited by 25 publications

(5 citation statements)

References 29 publications

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“…Most sweet potatoes with different symptoms reacted to SPFMV antiserum; however, there was little serological variation. This is consistent with the findings that amino acid and nucleotide sequences of CP genes are highly homologous among SPFMV-S, RC and C (Mori et al 1995); however, the amino acid sequence of the CP gene was not highly homologous in SPFMV-T and the other two strains (Sakai et al 1995). Nevertheless, resistance to the T strain was also displayed by the transgenic sweet potato.…”

Section: Discussionsupporting

confidence: 89%

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Evaluation of resistance to complex infection of SPFMVs in transgenic sweet potato

Okada

Saito

2008

Breed. Sci.

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Viral diseases of the sweet potato (Ipomoea batatas L. (Lam)) are highly prevalent and often cause serious damage to the plants, particularly the severe strain of the sweet potato feathery mottle virus (SPFMV-S) that causes 'obizyo-sohi' disease in Japan. To confer viral resistance to SPFMV using current biotechnology, a transgenic sweet potato has been developed by introducing SPFMV-S coat protein (CP) genes, which have shown significant resistance to SPFMV-S; however, ecological distribution in the field of SPFMVs is unclear. This study investigated the ecological distribution of SPFMVs in field-infected sweet potato, and virus resistance nearer to the field was evaluated in transgenic sweet potato. SPFMV was not capable of independent infection; rather, infection by SPFMVs was coincidentally complex under natural conditions. Furthermore, infection was by a dominant strain between complex infecting SPFMVs, and was differentiated by the area of infection. Transgenic sweet potato plants expressing the SPFMV-S CP gene were challenged by graft inoculation with field-infected SPFMVs. All of the transgenic sweet potato plants were highly protected in the long term against SPFMV complex infection compared to control plants. These results suggest that transgenic sweet potato can acquire resistance to SPFMVs in the field.

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Section: Discussionsupporting

confidence: 89%

“…Total RNA was extracted from the leaves by the cetyltrimethylammonium bromide method and reverse transcription was performed using primers designed in NIb-CP region of the Japanese isolates of SPFMV-O (Mori et al 1994), S (Mori et al 1995) and T (Sakai et al 1995). RT-PCR was performed using a modified method of .…”

Section: Introductionmentioning

confidence: 99%

Evaluation of resistance to complex infection of SPFMVs in transgenic sweet potato

Okada

Saito

2008

Breed. Sci.

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“…Plasmid construction DNA corresponding to the 5¢ 200 or 400 bp regions of CP of SPFMV were generated by polymerase chain reaction (PCR) from a cDNA clone, pVC1 (Mori et al 1995), using the specific forward primer, SPFMV.CP-F, 5¢-GGCCGGATCCAACAACAATGtctagtgaacgtactgaattcaaagatgcggga-3¢ and the reverse primers, SPFMV.CP-R200, 5¢-GCAGATCTGAGCTCgattccttctccttgctagtgtc-3¢ and SPFMV.CP-R400, 5¢-GCAGATCTGAGCTCgagtcgaccgggtgtttgcaacc-3¢. Letters in lower case represent CP sequence of SPFMV (accession number D 86371; 9652-9684 nt, 9828-9851 nt, 10028-10051 nt for SPFMVCP-F, SPFMV.CP-R200 and SPFMV.CP-R400, respectively).…”

Section: Plant Materialsmentioning

confidence: 99%

Analysis of transitive RNA silencing after grafting in transgenic plants with the coat protein gene of Sweet potato feathery mottle virus

et al. 2006

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We have previously reported the graft transmission of target specificity for RNA silencing using transgenic Nicotiana benthamiana plants expressing the coat protein gene (CP, including the 3' non-translated region) of Sweet potato feathery mottle virus. Transgenic plants carrying the 5' 200 and 400 bp regions of CP were newly produced. From these plants, two silenced and two non-silenced lines were selected to investigate the manifestation of transitive RNA silencing by graft experiments. Non-silenced scions carrying the entire transgene were grafted onto either 5' or 3' silencing inducer rootstocks. When non-silenced scions were grafted onto 5' silencing inducer rootstocks, RNA silencing was induced in the non-silenced scions and spread toward the 3' region of the transgene mRNA. Similarly, when non-silenced scions were grafted onto 3' silencing inducer rootstocks, RNA silencing was induced in the non-silenced scions, but was restricted to the 3' region of the transgene and did not spread to the 5' region. In addition, results from crossing experiments, involving non-silenced and 3' silencing inducer plants, confirmed the above finding. This indicates that RNA silencing spreads in the 5'-3' direction, not in the 3'-5' direction, along the transgene mRNA.

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“…We have obtained transgenic Kokei 14 plants possessing the coat protein gene of sweet potato feathery mottle virus [21], a severe pathogen of Kokei 14 in Japan. The A. tumefaciens-mediated gene transfer system using embryogenic callus may be useful as a routine method for the genetic modification of sweet potato.…”

Section: Discussionmentioning

confidence: 99%

Transgenic Plant Production from Embryogenic Callus of Sweet Potato (Ipomoea batatas (L.) Lam.) Using Agrobacterium tumefaciens

Otani¹,

Shimada²,

Kimura³

et al. 1998

Plant Biotechnology

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Nucleotide sequence analysis of two nuclear inclusion body and coat protein genes of a sweet potato feathery mottle virus severe strain (SPFMV-S) genomic RNA

Cited by 25 publications

References 29 publications

Evaluation of resistance to complex infection of SPFMVs in transgenic sweet potato

Evaluation of resistance to complex infection of SPFMVs in transgenic sweet potato

Analysis of transitive RNA silencing after grafting in transgenic plants with the coat protein gene of Sweet potato feathery mottle virus

Transgenic Plant Production from Embryogenic Callus of Sweet Potato (Ipomoea batatas (L.) Lam.) Using Agrobacterium tumefaciens

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