Nucleotide Sequence and Secondary Structure of Pome Fruit Viroids From Dapple Apple Diseased Apples, Pear Rusty Skin Diseased Pears and Apple Scar Skin Symptomless Pears

Zhu, Shuifang; Hadidi, A.; Xicai, Yang; Hammond, R. W.; Hansen, A. J.

doi:10.17660/actahortic.1995.386.80

Cited by 23 publications

(10 citation statements)

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“…Later it was suggested that dapple apple disease is caused by a variant of ASSVd (Hadidi et al, 1991). Two other diseases, pear rusty skin (Chen et al, 1986) and Japanese pear fruit dimple (Osaki et al, 1996) have also been attributed to ASSVd molecular variants (Zhu et al, 1995). In the last 20 years, ASSVd has been reported in pear (Pyrus communis, Pyrus pyrifolia), wild apple (Malus sylvestris) and wild pear (Pyrus amygdaliformis; Kyriakopoulou et al, 2001Kyriakopoulou et al, , 2003Koganezawa et al, 2003;Boubourakas et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

Identification of the herbaceous host range of Apple scar skin viroid and analysis of its progeny variants

et al. 2013

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Apple scar skin viroid (ASSVd) is the type member of the genus Apscaviroid of the family Pospiviroidae and is known to cause scarring and dappling of apple fruits. The herbaceous host range of ASSVd was identified using an infectious dimeric clone. Among 13 plant species inoculated with the viroid, nine were found to be systemic hosts and three served as indicator hosts. The viroid could be transferred to these hosts through agroinoculation, rubbing of dimeric RNA transcripts, dimeric DNA plasmids and through sap inoculation. Single-strand confirmation polymorphism (SSCP) analysis of the progeny obtained after inoculation of the infectious ASSVd clone showed the presence of four sequence variants (V1, V2, V3 and V4) which was also confirmed by sequencing. Two sequence variants (V1 and V3) were identified as dominant, representing 47Á72 and 36Á36% of the total progeny, respectively. The V4 sequence variant was similar to the wild type and occurred at a frequency of only 4Á5% of the total progeny.

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Section: Introductionmentioning

confidence: 99%

Identification of the herbaceous host range of Apple scar skin viroid and analysis of its progeny variants

et al. 2013

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“…This disease was initially attributed to the same causal agent of apple scar skin disease [ 55 ] and, after molecular characterization, to the same viroid [ 38 ]. More specifically, ASSVd variants of 331 nt were found to be closely associated with dapple apple symptoms [ 34 ]. Successful systemic infection of apple and pear seedlings was obtained by agroinoculation of a dimeric head-to-tail cDNA of ASSVd isolated from fruits affected by dapple apple disease [ 35 ].…”

Section: Viroid Diseases Caused By Apple Scar Skin Viroid (Assvd)mentioning

confidence: 99%

Viroid Diseases in Pome and Stone Fruit Trees and Koch’s Postulates: A Critical Assessment

Serio

Ambrós

Shimmen

et al. 2018

Viruses

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Composed of a naked circular non-protein-coding genomic RNA, counting only a few hundred nucleotides, viroids—the smallest infectious agents known so far—are able to replicate and move systemically in herbaceous and woody host plants, which concomitantly may develop specific diseases or remain symptomless. Several viroids have been reported to naturally infect pome and stone fruit trees, showing symptoms on leaves, fruits and/or bark. However, Koch’s postulates required for establishing on firm grounds the viroid etiology of these diseases, have not been met in all instances. Here, pome and stone fruit tree diseases, conclusively proven to be caused by viroids, are reviewed, and the need to pay closer attention to fulfilling Koch’s postulates is emphasized.

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“…250-400 nucleotides(nt) RNA , (Diener, 1987;Di Serio , 1996;Hashimoto Koganezawa, 1987). ASSVd , , , (Hashimoto Koganezawa, 1987;Zhu , 1995). ASSVd 1935 (Ohtsuka, 1938), , , , (Cambell Sparks, 1976;Diener, 1987;Millikan Martin, 1956;Yamaguchi Yanase, 1976).…”

Section: Apple Mosaic Virus Apple Chlorotic Leaf Spot Virus Apple Smentioning

confidence: 99%

Phylogenetic Analysis of Apple scar skin viroid Isolates in Korea

Cho¹,

Kim²,

Kil³

et al. 2015

Research in Plant Disease

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To identify genome sequences of Apple scar skin viroid (ASSVd) isolates in Korea, the field survey was performed from 'Hongro' apple orchards located in eight sites in South Korea (Bongwha, Cheongsong, Dangjin, Gimchoen, Muju, Mungyeong, Suwon, and Yeongwol). ASSVd was detected by RT-PCR and PCR fragments were cloned into cloning vector. Full-length viral genomes of eight ASSVd isolates were sequenced and compared with 21 isolates reported previously from Korea, India, China, Japan and Greece. Eight isolates in this study showed 92.2-99.7% nucleotide sequence identities with those reported previously. Phylogenetic analysis showed that seven isolates reported in this study belong to the same group distinct from other groups.

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Nucleotide Sequence and Secondary Structure of Pome Fruit Viroids From Dapple Apple Diseased Apples, Pear Rusty Skin Diseased Pears and Apple Scar Skin Symptomless Pears

Cited by 23 publications

References 0 publications

Identification of the herbaceous host range of Apple scar skin viroid and analysis of its progeny variants

Identification of the herbaceous host range of Apple scar skin viroid and analysis of its progeny variants

Viroid Diseases in Pome and Stone Fruit Trees and Koch’s Postulates: A Critical Assessment

Phylogenetic Analysis of Apple scar skin viroid Isolates in Korea

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