Nucleotide sequence and translation of satellite tobacco mosaic virus RNA

Mirkov, T. Erik; Mathews, D. M.; Plessis, D.H. Du; Dodds, J. A.

doi:10.1016/0042-6822(89)90361-9

Cited by 71 publications

(54 citation statements)

References 31 publications

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“…The 240 3'-terminal nucleotides of STMV RNA exhibit considerable sequence similarity with the Y-terminal part of TMGMV and U1-TMV RNAs. However, such a similarity was not seen (Mirkov et al, 1989) between the coding regions of STMV and tobamoviral RNAs. Also, in contrast to the rod-shaped structure of TMV, STMV has a spherical morphology.…”

mentioning

confidence: 89%

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Similarities between the secondary structure of satellite tobacco mosaic virus and tobamovirus RNAs

Gultyaev

Batenburg

Pleij

1994

Journal of General Virology

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confidence: 89%

“…Its genome consists of ssRNA of 1059 nucleotides (Mirkov et al, 1989). STMV is also maintained by other tobamoviruses, including tobacco mosaic virus (U 1-TMV), although these helpers are less efficient.…”

mentioning

confidence: 99%

Similarities between the secondary structure of satellite tobacco mosaic virus and tobamovirus RNAs

Gultyaev

Batenburg

Pleij

1994

Journal of General Virology

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“…The structure of satellite tobacco mosaic virus (STMV), a T = 1 icosahedral particle of 17 nm diameter, has a singlestranded (ss) RNA genome of 1058 nucleotides [1]. Secondary structure prediction [2,3] indicates possible base pairing approaching 70%, a feature shared by other satellite viruses and satellite RNAs [4][5][6].…”

Section: Introductionmentioning

confidence: 99%

Satellite tobacco mosaic virus RNA: structure and implications for assembly

Larson

McPherson

2001

Current Opinion in Structural Biology

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“…The five viruses whose RNA we extracted and visualized are poliovirus, [15][16][17] TYMV, [18][19][20] brome mosaic virus (BMV), [21][22][23] STMV, 3,4,[24][25][26][27] which are all icosahedral viruses, and tobacco mosaic virus (TMV), [28][29][30][31] which is a helical, rod-shaped virus. We included TMV because its RNA molecules do not share the secondary or tertiary structural requirements of those of the icosahedral viruses.…”

Section: Introductionmentioning

confidence: 99%

Atomic Force Microscopy Analysis of Icosahedral Virus RNA

Kuznetsov

Daijogo

Zhou

et al. 2005

Journal of Molecular Biology

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Single-stranded genomic RNAs from four icosahedral viruses (poliovirus, turnip yellow mosaic virus (TYMV), brome mosaic virus (BMV), and satellite tobacco mosaic virus (STMV)) along with the RNA from the helical tobacco mosaic virus (TMV) were extracted using phenol/chloroform. The RNAs were imaged using atomic force microscopy (AFM) under dynamic conditions in which the RNA was observed to unfold. RNAs from the four icosahedral viruses initially exhibited highly condensed, uniform spherical shapes with diameters consistent with those expected from the interiors of their respective capsids. Upon incubation at 26 8C, poliovirus RNA gradually transformed into chains of globular domains having the appearance of thick, irregularly segmented fibers. These ultimately unwound further to reveal segmented portions of the fibers connected by single strands of RNA of 0.5-1 nm thickness. Virtually the same transformations were shown by TYMV and BMV RNA, and with heating, the RNA from STMV. Upon cooling, the chains of domains of poliovirus RNA and STMV RNA condensed and re-formed their original spherical shapes. TMV RNAs initially appeared as single-stranded threads of 0.5-1.0 nm diameter but took on the structure of the multidomain chains upon further incubation at room temperature. These ultimately condensed into short, thick chains of larger domains. Our observations suggest that classical extraction of RNA from icosahedral virions produces little effect on overall conformation. As tertiary structure is lost however, it is evident that secondary structural elements are arranged in a sequential, linear fashion along the polynucleotide chain. At least in the case of poliovirus and STMV, the process of tertiary structure re-formation from the linear chain of secondary structural domains proceeds in the absence of protein. RNA base sequence, therefore, may be sufficient to encode the conformation of the encapsidated RNA even in the absence of coat proteins.

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Nucleotide sequence and translation of satellite tobacco mosaic virus RNA

Cited by 71 publications

References 31 publications

Similarities between the secondary structure of satellite tobacco mosaic virus and tobamovirus RNAs

Similarities between the secondary structure of satellite tobacco mosaic virus and tobamovirus RNAs

Satellite tobacco mosaic virus RNA: structure and implications for assembly

Atomic Force Microscopy Analysis of Icosahedral Virus RNA

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