Nucleotide Sequence-Based Analysis for Determining the Molecular Epidemiology of
            <i>Penicillium marneffei</i>

Lasker, Brent A.

doi:10.1128/jcm.00567-06

Cited by 16 publications

(14 citation statements)

References 58 publications

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“…The only locus that showed significant variation was MP1, which is in line with the results observed in the present study, that only the MP1 homologues, but not the 11 housekeeping genes, showed significant variation. In the present study, the region of the MP1 gene used was from nucleotide 57 to 626, whereas in the other study (21), the region used was from nucleotide 508 to 957, using strain PM4 as the reference. This is probably the explanation of the higher variation in the MP1 gene in the present study.…”

Section: Discussionmentioning

confidence: 96%

“…Using two times the number of human isolates, we found that the present MLST system appears to be more discriminative than another MLST system recently published for molecular typing of P. marneffei (21). In the recently published article on nucleotide sequence-based analysis of P. marneffei isolates, nine gene loci were sequenced (21).…”

Section: Discussionmentioning

confidence: 99%

“…In the recently published article on nucleotide sequence-based analysis of P. marneffei isolates, nine gene loci were sequenced (21). Among these nine loci, five showed sequence variations.…”

Section: Discussionmentioning

confidence: 99%

“…MPI and PMATPase were used in the MLST scheme of Candida albicans; PK, GDH, and PGM were used in the multilocus enzyme electrophoresis scheme of C. albicans; CCR and TOP2 were known housekeeping genes of Penicillium verruculosum and Talaromyces flavus, respectively; and rNDP reductase, GltS, RNR, and PacC were genes found in our random exploration of the P. marneffei genome project (29,30,33,47). None of these seven genes was the same as the four housekeeping genes chosen for the recently published nucleotide sequence-based system for typing P. marneffei (21). The 14 lineage-specific genes include MP1 and its homologues that belong to a novel mannoprotein superfamily.…”

Section: Methodsmentioning

confidence: 99%

“…As multilocus sequence typing (MLST) is well known to be highly reproducible and discriminative for bacteria and has also been used for a number of fungal pathogens, we started to develop such a typing system for P. marneffei 3 years ago. Recently, Lasker has published a nucleotide sequence-based system for typing P. marneffei (21). In this article, we report the difficulty in using housekeeping genes and the subsequent success in using lineage-specific loci-a set of homologous genes belonging to a novel mannoprotein superfamily-for MLST in P. marneffei.…”

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confidence: 99%

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MP1 Homologue-Based Multilocus Sequence System for Typing the Pathogenic Fungus Penicillium marneffei : a Novel Approach Using Lineage-Specific Genes

et al. 2007

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A highly reproducible and discriminative typing system is essential for better understanding of the epidemiology of Penicillium marneffei, the most important thermal dimorphic fungus causing respiratory, skin, and systemic mycosis in Southeast Asia. The sequences of 11 housekeeping genes were identical among 10 strains of P. marneffei, but those of MP1 and its 13 homologues, a novel superfamily of mannoproteins in the subdivision Pezizomycotina of Ascomycetes, mostly species of Penicillium and Aspergillus, showed significant variations. Therefore, a multilocus sequence typing (MLST) system for P. marneffei was constructed using MP1

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Section: Discussionmentioning

confidence: 96%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

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MP1 Homologue-Based Multilocus Sequence System for Typing the Pathogenic Fungus Penicillium marneffei : a Novel Approach Using Lineage-Specific Genes

et al. 2007

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Penicilliosis

2011

Fundamental Medical Mycology

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Aspergillus , Talaromyces , and Penicillium

Chen,

Meyer,

Sorrell

et al. 2023

ClinMicroNow

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The genus Aspergillus is classified in the family Trichocomaceae of the Ascomycota. The majority of human infections due to known Aspergillus species are caused by A. fumigatus , followed by A. flavus , A. terreus , and A. niger , with recognition that these designations represent complexes of closely related species. Aspergillus species are environmental saprophytes that thrive in decaying vegetation and are readily cultured from soil, water, certain foods, and air. Aspergillus DNA can be detected in clinical specimens either by nucleic acid amplification methods specifically targeting Aspergillus or by panfungal PCR assays followed by DNA sequencing for species identification. Penicillium sensu lato is associated with two teleomorphic genera: Eupenicillium and Talaromyces . Assays have been developed to detect antibodies to Talaromyces marneffei , Talaromyces marneffei antigen, or both in serum and other body fluids in various test formats, including immunodiffusion, indirect immunofluorescence, and enzyme‐linked immunosorbent assay.

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Nucleotide Sequence-Based Analysis for Determining the Molecular Epidemiology of Penicillium marneffei

Cited by 16 publications

References 58 publications

MP1 Homologue-Based Multilocus Sequence System for Typing the Pathogenic Fungus Penicillium marneffei : a Novel Approach Using Lineage-Specific Genes

MP1 Homologue-Based Multilocus Sequence System for Typing the Pathogenic Fungus Penicillium marneffei : a Novel Approach Using Lineage-Specific Genes

Penicilliosis

Aspergillus , Talaromyces , and Penicillium

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