Nucleotide sequence of a human gene for glutathione peroxidase

Ishida, Koichi; Morino, Tomio; Takagi, Kenkichi; Sukenaga, Yoshikazu

doi:10.1093/nar/15.23.10051

Cited by 50 publications

(21 citation statements)

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“…Mammalian glutathione peroxidase [36,37] and E. coli formate dehydrogenase [38] both contain a UGA codon interrupting the ORF and encoding the Se-Cys found in the active site of both enzymes. Se-Cys is cotranslationally incorporated into proteins [38,39] and it has been shown that the Se-Cys backbone in the glutathione peroxidase arises from Ser [40].…”

Section: Nonsense Suppression Via Misreadingmentioning

confidence: 99%

Stop making sense or Regulation at the level of termination in eukaryotic protein synthesis

Valle

Morch

1988

FEBS Letters

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Section: Nonsense Suppression Via Misreadingmentioning

confidence: 99%

Stop making sense or Regulation at the level of termination in eukaryotic protein synthesis

Valle

Morch

1988

FEBS Letters

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“…This enzyme is encoded by the GPx1 gene, which is located at chromosome 3p21 and contains two exons within a 1.42 kb region [25]. A transition of C to T (rs:1050450) is known to exist at nucleotide 594 in exon 2 of the GPx1 gene, corresponding to an amino acid change from proline (Pro) to leucine (Leu) at codon 198 (Pro198Leu) [26]. Selenium enhances GPX1 expression and increases cellular antioxidant capacity.…”

Section: Introductionmentioning

confidence: 99%

The role of Cat -262C/T, GPX1 Pro198Leu and Sod1+35A/C gene polymorphisms in a development of primary open-angle glaucoma in a Polish population

Malinowska¹,

Kowalski²,

Szaflik³

et al. 2016

pjp

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The development of glaucoma may be connected with a long-term exposure to oxidative stress caused by free radical (ROS). The main aim of this work was an analysis of associations of Cat-262C/T, GPX1 Pro198Leu and SOD1 35 A/C gene polymorphisms of antioxidant enzymes with a risk of open-angle glaucoma (POAG) in a Polish population. DNA samples collected from 209 patients with POAG and 191 healthy controls were used in this study. Polymorphisms were determined by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). We found that the +35A/C polymorphisms of SOD1 were not associated with a risk of POAG. We found that C/T genotype (1-GPX1Pro198Leu, 2-Cat C262T) is associated with increased risk of open angle glaucoma (1-OR = 2.24; 95% CI: 1.46-3.44; p = 0.0001, 2-OR = 2.16; 95% CI: 1.35-3.34; p = 0.001). We also found that T/T genotype is a risk factor for progression of POAG (1-OR = 3.86; 95% Cl: 1.36-10.96; p = 0.007, 2-OR = 6.37; 95% CI: 1.39-29.28; p = 0.007). Finally our data suggest that gene polymorphisms of GPX1 Pro198Leu and CAT C262T may have a protective role in the development of primary open-angle glaucoma in a Polish population.

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“…Selenium presents as selenocysteine (Sty) in some seleno-proteins, such as cellular glutathione peroxidase (GPx) [2], plasma glutathione peroxidase 133, S'-iodothyronine monodeiodinase (S'DI) [4], plasma seleno-protein (SeP) [S], and phospholipid hydroperoxide glutathione peroxidase (PHGPx) [6]. We previously reported the sequences of human cellular GPx cDNA and genomic DNA [7,8]. The Sty codon on mRNAs of these proteins is UGA which also functions as a stop codon.…”

Section: Introductionmentioning

confidence: 99%

“…However, it is possible that a key structure for recognition of the Sty UGA codon presents in the frame of mRNAs of mammalian selenoproteins and is located near the Sty UGA codon. In [20], Human GPx genes used were the cDNA and gcnomic DNA previously reported [7,8]. Genomic DNA and cDNA of GPx were subcloned in pcD [21] or pSV [22] which contain the SV40 ori and early promoter.…”

Section: Introductionmentioning

confidence: 99%

The 5′ untranslated region of the human cellular glutathione peroxidase gene is indispensable for its expression in COS‐7 cells

et al. 1992

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WC studied the expression of the human cellular glutathionc peroxidase (GPx) gene, from which a key enzyme containing sclenocysteine (Sty) at the active site is produced. Expression of some human GPx gene mutants in COS-7 cells revealed that the 5' untranslated region (utr) was necessary for expression of the GPx gene, since mutant genes having 10 base pairs (bps) at the S'utr (the complete had 311 bps) expressed GPx at very low levels. The genes with 31 I or 405 bps at the S'utr wcrc better expressed than those having 257 bps. The GPx gene having 133 bps at the 3'utr (80 bps shorter than the entire length) was highly expressed. This deletion did not influence expression. We constructed some mutants in which 3 bases were altered at the upstream region of the Sty UGA codon i. *. the frame ,of the GPx Ben.+ by site-directed mutagenesis. GPx expression decreased but the expression was restored. Therefore, the upstream region of the in-frame Sty codon was nL?t essential in the Sty decoding mechanisms. Finally, the S'utr was essential for the expression of GPx gene. However, the deletion of a part of the 3'utr and the siw-directed mutation upstream of the Sty codon did not show drastic effects on the expression.

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Nucleotide sequence of a human gene for glutathione peroxidase

Cited by 50 publications

References 1 publication

Stop making sense or Regulation at the level of termination in eukaryotic protein synthesis

Stop making sense or Regulation at the level of termination in eukaryotic protein synthesis

The role of Cat -262C/T, GPX1 Pro198Leu and Sod1+35A/C gene polymorphisms in a development of primary open-angle glaucoma in a Polish population

The 5′ untranslated region of the human cellular glutathione peroxidase gene is indispensable for its expression in COS‐7 cells

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