Nucleotide sequence of acceptor site and termini of integrated avian endogenous provirus ev1: Integration creates a 6 bp repeat of host DNA

Hishinuma, Fumio; DeBona, P; Astrin, Susan M.; Skalka, A M

doi:10.1016/0092-8674(81)90280-4

Cited by 131 publications

(86 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…LTRs contain important regulatory signals for the promotion, initiation, and processing of viral mRNAs and may play a role in the integration of proviral DNA into the host chromosome (3). Furthermore, nucleotide sequence analyses show that LTRs possess structural features characteristic of transposable elements found in bacteria, yeast, and Drosophila (4)(5)(6)(7)(8)(9)(10)(11).In several vertebrate species, DNA copies of type C retroviruses have been identified as genetically stable integral components of chromosomal DNA (12). Such endogenous proviruses have been shown to be vertically transmitted, can be mapped to specific chromosomal loci, and, in some cases, may be expressed as infectious retroviruses (12).…”

mentioning

confidence: 99%

Endogenous murine leukemia proviral long terminal repeats contain a unique 190-base-pair insert.

Khan¹,

Martin²

1983

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

We have determined the nucleotide sequence in the U3-R regions of the long terminal repeat (LTR) associated with NFS-Th-I xenotropic murine leukemia virus (MuLV) DNA The RNA genome of type C retroviruses contains terminally redundant sequences (R) that are located adjacent to segments unique to the 5' (U5) and 3' (U3) termini (1). After infection, the viral RNA is reverse transcribed into double-stranded DNA, which subsequently becomes integrated into cellular chromosomal DNA (2, 3). Both the unintegrated and integrated copies of proviral DNA contain terminally duplicated sequences derived from each end of the viral genome forming a structure (U3-R-U5) referred to as the long terminal repeat (LTR). LTRs contain important regulatory signals for the promotion, initiation, and processing of viral mRNAs and may play a role in the integration of proviral DNA into the host chromosome (3). Furthermore, nucleotide sequence analyses show that LTRs possess structural features characteristic of transposable elements found in bacteria, yeast, and Drosophila (4)(5)(6)(7)(8)(9)(10)(11).In several vertebrate species, DNA copies of type C retroviruses have been identified as genetically stable integral components of chromosomal DNA (12). Such endogenous proviruses have been shown to be vertically transmitted, can be mapped to specific chromosomal loci, and, in some cases, may be expressed as infectious retroviruses (12). We Because we had previously shown that the LTRs associated with endogenous MuLV proviruses could be distinguished from the LTR segments associated with known infectious viruses (13) and since the numerous copies of these elements present in mammalian chromosomal DNA could potentially regulate the expression of adjacent cellular genes, we determined the nucleotide sequences in the U3 R regions of five endogenous LTRs. The results of these sequence analyses and comparisons with LTRs associated with infectious xenotropic, ecotropic, and MCF proviruses are presented.MATERIALS AND METHODS Recombinant DNA Clones. The Pst I/Sma I segment derived from the U3-R region of the LTRs associated with the endogenous BALB/c MuLV proviral DNA clones B-56, B-73, B-14 (5' LTRs), and B-34 (3' LTR) (13) and with NFS-Th-1 xenotropic DNA cloned from MuLV-infected cells (14) The publication costs ofthis article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. §1734 solely to indicate this fact.

show abstract

mentioning

confidence: 99%

Endogenous murine leukemia proviral long terminal repeats contain a unique 190-base-pair insert.

Khan¹,

Martin²

1983

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

show abstract

“…In rat cells, Rous sarcoma virus (Hughes et al, 1981) and MMTV (Majors & Varmus, 1981) generate 6 bp repeats, Mo-MuLV causes a 4 bp repeat (Shoemaker et al, 1981), and SNV causes a 5 bp repeat. Furthermore, in chickens ev-1 has a 6 bp direct repeat (Hishinuma et al, 1981). Thus from virus to virus in one cell type the repeat size is different, but for one virus, SNV, in two cell types the repeat size is the same.…”

Section: Sequence Of Dna Near Unoccupied Integration Sitementioning

confidence: 99%

“…Structural and sequence analysis of the LTRs of several different retroviruses (Dhar et al, 1980;Ju & Skalka, 1980;Reddy et al, 1980;Sutcliffe et al, 1980;Van Beveren et al, 1980;Yamamoto et al, 1980;Donehower et al, 1981 ;Swanstrom et al, 1981) and the observation that proviruses generate short direct repeats of host DNA upon insertion Van Beveren et al, 1982;Hishinuma et al, 1981 ;Hughes et al, 1981 ;Majors & Varmus, 1981 ;Shoemaker et al, 1981) have revealed a remarkable resemblance between retroviruses and transposable elements (Calos & Miller, 1980). It has been proposed that the mechanism of integration of retroviruses may be similar to transposition and that retroviruses evolved from cellular movable genetic elements .…”

Section: Introductionmentioning

confidence: 99%

Cellular DNA Surrounding Integration Sites of an Avian Retrovirus

Fitts

Temin

1983

Journal of General Virology

View full text Add to dashboard Cite

267 SUMMARYThe size of the direct repeats of cellular DNA next to a spleen necrosis virus (SNV) provirus from infected rat cells and the nature of the cellular DNA surrounding SNV integration sites in chicken DNA were studied. A five-base pair repeat, ATTTT, was observed at the SNV-rat cell junctions. Five of ten SNV proviruses from chicken cells were flanked by unique DNA and five others were flanked by repetitive DNA. No large rearrangements of cellular DNA at SNV integration sites were observed. A clone of uninfected chicken DNA containing the presumptive unoccupied integration site for one SNV provirus was obtained and part of it was sequenced. A small substitution, approximately 60 nucleotides, was observed at the site of virus DNA insertion. Its significance is not known.

show abstract

“…All of these elements are a few kilobases (kb) long, they are flanked by either direct or inverted terminal repeats and they generate short duplications in target DNA as a consequence of integration (e.g. 4,5,6). Some of the eukaryotic transposons and retroviral proviruses have further similarities.…”

Section: Introductionmentioning

confidence: 99%

Expression of Ty-lacZfusions inSaccharomyces cerevisiae

et al. 1984

View full text Add to dashboard Cite

We have determined the nucleotide sequence of about 520 bp spanning the 5' delta regions (Figure 1) of two Tyl elements. There is an open reading frame running out of the deltas for at least 180 nucleotides into the internal region of each element. The functional significance of these open reading frames has been tested by fusing them to a defective E.coli lacZ gene. Expression of B-galactosidase in yeast transformants containing these fusions shows that Tyl elements contain functional translation signals.

show abstract

Nucleotide sequence of acceptor site and termini of integrated avian endogenous provirus ev1: Integration creates a 6 bp repeat of host DNA

Cited by 131 publications

References 41 publications

Endogenous murine leukemia proviral long terminal repeats contain a unique 190-base-pair insert.

Endogenous murine leukemia proviral long terminal repeats contain a unique 190-base-pair insert.

Cellular DNA Surrounding Integration Sites of an Avian Retrovirus

Expression of Ty-lacZfusions inSaccharomyces cerevisiae

Contact Info

Product

Resources

About