Nucleotide sequence of bacteriophage φX174 DNA

Sanger, F.; Air, Gillian M.; Barrell, Barclay G.; Brown, Nigel L.; Coulson, Alan; Fiddes, John C.; Hutchison, Clyde A.; Slocombe, Patrick M.; Smith, Michael J.

doi:10.1038/265687a0

Cited by 1,737 publications

(812 citation statements)

References 52 publications

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“…Plasmid construction pXL2776 was constructed from pXL2675, described by Wils and Ollivier in 1996 (patent reference FR 9603519): the minimal attB site 7 was inserted between the HindIII and SacI sites, and the attP site (position 27480 to 27860 in the published sequence of Sanger et al 24 ) was inserted between the XbaI and NsiI sites. Thus, pXL2776 contained a multiple cloning site between the attP and attB sites in the same orientation, and a kanamycin expression cassette.…”

Section: Methodsmentioning

confidence: 99%

Minicircle: an improved DNA molecule for in vitro and in vivo gene transfer

et al. 1999

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Minicircles are a new form of supercoiled DNA molecule for enhancer/promoter. Comparing maximal differences, these nonviral gene transfer which have neither bacterial origin of minicircles gave 2.5 to 5.5 times more reporter gene replication nor antibiotic resistance marker. They are thus activity than the unrecombined plasmid in the NIH3T3 cell smaller and potentially safer than the standard plasmids line and rabbit smooth muscle cells. Moreover, injection in currently used in gene therapy. They were obtained in E.vivo into mouse cranial tibial muscle, or human head and coli by att site-specific recombination mediated by the neck carcinoma grafted in nude mice resulted in 13 to 50 phage integrase, which was used to excise the times more reporter gene expression with minicircles than expression cassette from the unwanted plasmid with the unrecombined plasmid or larger plasmids. Histosequences. We produced two minicircles containing the logical analysis in muscle showed there were more transluciferase or ␤-galactosidase gene under the control of fected myofibers with minicircles than with unrecombined the strong human cytomegalovirus immediate-early plasmid.

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Section: Methodsmentioning

confidence: 99%

Minicircle: an improved DNA molecule for in vitro and in vivo gene transfer

et al. 1999

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“…Combination of the maps of Fig. 4 and the genetic map of 1$X174 (Weisbeek et al, 1976;Sanger et al, 1977) and the start of gene E of G4. The outer fragment map represents the 4X174 AluI map, the next map is the genetic map of +X174.…”

Section: Discussionmentioning

confidence: 99%

“…The physical-genetic map of +X174 phage has been well established (Weisbeek et al, 1976;Sanger et al, 1977) and the present study was undertaken to align the G4 and $X174 restriction enzyme cleavage maps and to obtain information on the position and order of the genes of G4 phage on the physical map.…”

Section: Introductionmentioning

confidence: 99%

Comparison of the G4 and φX174 phage genomes by electron microscopy

et al. 1979

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The order of G4 Haemophilus in$uenzae Rd (HindII) DNA fragments was mapped by electron microscopy. G4 Hind11 DNA fragments were annealed to 4x1'74 viral singlestranded DNA circles and found to partially hybridize. Eight to eighteen percent of the 4x1'74 DNA circles had a G4 DNA fragment attached, depending upon the fragment and the time of annealing. The G4 Hind11 restriction enzyme cleavage map was aligned with the $X174 restriction enzyme cleavage map by analyzing under the electron microscope partial heteroduplex molecules consisting of +X174 single-stranded viral DNA circles annealed with 4X174 Arthrobacter luteus (AluI) DNA fragments as markers and G4 Hind11 DNA fragments. These results indicate that the gene order of the bacteriophages G4 and 1$X174 is the same. Despite the high frequency of 4X174/G4 heteroduplex DNA molecules observed under the electron microscope, heterologous marker rescue experiments with G4 DNA fragments and 1$X174 mutant viral DNA strand circles were not successful, except in the case of a +X174 amber mutation in gene E (am3).

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“…Genomics emerged with the sequencing of the first complete genome, bacteriophage jX174, by Fredrick Sanger in 1977, spanning approximately 5 kb encompassing just 10 genes (Sanger et al, 1977). Since then genomic sequencing has rapidly progressed and complete genomes are now known for many The paradigm shift in approach to animal breeding.…”

Section: Genomicsmentioning

confidence: 99%

The integration of ‘omic’ disciplines and systems biology in cattle breeding

Berry

Meade

Mullen

et al. 2011

Animal

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Enormous progress has been made in the selection of animals, including cattle, for specific traits using traditional quantitative genetics approaches. Nevertheless, considerable variation in phenotypes remains unexplained, and therefore represents potential additional gain for animal production. In addition, the paradigm shift in new disciplines now being applied to animal breeding represents a powerful opportunity to prise open the 'black box' underlying the response to selection and fully understand the genetic architecture controlling the traits of interest. A move away from traditional approaches of animal breeding toward systems approaches using integrative analysis of data from the 'omic' disciplines represents a multitude of exciting opportunities for animal breeding going forward as well as providing alternatives for overcoming some of the limitations of traditional approaches such as the expressed phenotype being an imperfect predictor of the individual's true genetic merit, or the phenotype being only expressed in one gender or late in the lifetime of an animal. This review aims to discuss these opportunities from the perspective of their potential application and contribution to cattle breeding. Harnessing the potential of this paradigm shift also poses some new challenges for animal scientists -and they will also be discussed.

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Nucleotide sequence of bacteriophage φX174 DNA

Cited by 1,737 publications

References 52 publications

Minicircle: an improved DNA molecule for in vitro and in vivo gene transfer

Minicircle: an improved DNA molecule for in vitro and in vivo gene transfer

Comparison of the G4 and φX174 phage genomes by electron microscopy

The integration of ‘omic’ disciplines and systems biology in cattle breeding

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