Nucleotide sequence of infectious bursal disease virus genome segment A delineates two major open reading frames

Spies, U.; Müller, Hermann; Becht, H.

doi:10.1093/nar/17.19.7982

Cited by 64 publications

(41 citation statements)

References 3 publications

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“…Sequence data were aligned by the computer program DNASIS (Pharmacia). As an alternative technique sequences inserted into the pUC vector were determined as described (Spies et al, 1989). This protocol and the asymmetric PCR technique confirmed the results obtained by direct PCR sequencing.…”

Section: Methodsmentioning

confidence: 60%

“…The forward primer for reverse transcription and PCR corresponded to base pairs 65 to 82 upstream of the initiation codon of the VP2 gene, and the backward primer covered the positions 1473 to 1456 in the VP4 gene immediately adjacent to the VP2 region. These numbers of base pairs correspond to the positions proposed by Spies et al (1989) and Hudson et al (1986). For the sequencing reactions two additional pairs of primers were chosen: no.…”

Section: Methodsmentioning

confidence: 99%

“…In some experiments asymmetric PCR was employed (lnnis et al, 1988;Innis & Gelfand, 1990). Positive controls contained eDNA spanning the entire VP2 gene of wild-type Cu-1 (Spies et al, 1989;Bayliss et al, 1990).…”

Section: Methodsmentioning

confidence: 99%

“…(Spies et al, 1989;Bayliss et al, 1990). This P C R amplification p r o d u c t furnished the template for the direct establishment of the nucleotide sequence o f the five escape m u t a n t s and o f the serotype II strain 23/82.…”

Section: Preparation Of Cdna Of the Vp2 Genementioning

confidence: 99%

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The genetic basis for the antigenicity of the VP2 protein of the infectious bursal disease virus

Schnitzler

Bernstein

Müller

et al. 1993

Journal of General Virology

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111

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The genomic region coding for the antigenic structure responsible for the induction of neutralizing antibodies was localized in the central variable region of the VP2 gene by comparing the nucleotide sequence of five escape mutants derived from the standard infectious bursal disease virus strain Cu-1. Exchange of a single amino acid at one of the prominent hydrophilic parts of this region proved to be sufficient for altering the neutralizing properties. The reactivity of neutralizing antibodies with peptides expressed in vitro encompassing both hydrophilic areas suggests that the entire variable region is engaged in the formation of this conformationdependent antigenic site. VP2-specific, non-neutralizing monoclonal antibodies directed against the sequencedependent epitope of the serotype I strain Cu-1 and the serotype II strain 23/82 cross-reacted with peptides located towards the carboxy terminus of VP2; no reaction occurred with peptides derived from the aminoterminal side adjacent to the variable region.

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Section: Methodsmentioning

confidence: 60%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Preparation Of Cdna Of the Vp2 Genementioning

confidence: 99%

See 2 more Smart Citations

The genetic basis for the antigenicity of the VP2 protein of the infectious bursal disease virus

Schnitzler

Bernstein

Müller

et al. 1993

Journal of General Virology

Self Cite

111

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“…IBDV segment A was shown to encompass one large open reading frame (ORF) which encodes a 110 kDa primary translation product. This precursor polyprotein is processed into three mature viral proteins (VP), VP2, VP3 and VP4 (Hudson et al, 1986;Spies et al, 1989). VP2 and VP3 are the major structural proteins of IBDV, whereas VP4 most likely represents a putative virus-encoded protease (Azad et al, 1987;Jagadish et aL, 1988 (Azad et al, 1985).…”

Section: Infectious Bursal Disease Virus (Ibdv) Causes An Immunosupprmentioning

confidence: 99%

Identification of a novel viral protein in infectious bursal disease virus-infected cells

Mundt¹,

Beyer²,

Müller

1995

Journal of General Virology

213

106

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Characterisation of an Indonesian very virulent strain of infectious bursal disease virus

et al. 2002

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An Indonesian very virulent (vv) strain of infectious bursal disease virus (IBDV), designated Tasik94, was characterised both in vivo and at the molecular level. Inoculation of Tasik94 into 5-week-old specific-pathogen-free (SPF) chickens resulted in 100% morbidity and 45% mortality. The complete nucleotide and predicted amino acid sequences of genomic segments A and B were determined. Across each of the three deduced open reading frames (ORFs), Tasik94 shared the greatest nucleotide homology to Dutch vv strain D6948. Phylogenetic analyses were performed using 15 full-length polyprotein sequences and a total of 105 VP2 hypervariable region sequences from geographically and pathogenically diverse strains. In each case, Tasik94 grouped closely with vv strains, particularly those from Europe. The deduced VP1, VP2, VP3, VP4 and VP5 protein sequences of Tasik94 were aligned with those from published strains and putative virulence determinants were identified in VP2, VP3 and VP4. Alignment of additional protein sequences across the VP2 hypervariable region confirmed that residues Ile[242], Ile[256] and Ile[294] were highly-conserved amongst vv strains, and may account for their enhanced virulence.

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Nucleotide sequence of infectious bursal disease virus genome segment A delineates two major open reading frames

Cited by 64 publications

References 3 publications

The genetic basis for the antigenicity of the VP2 protein of the infectious bursal disease virus

The genetic basis for the antigenicity of the VP2 protein of the infectious bursal disease virus

Identification of a novel viral protein in infectious bursal disease virus-infected cells

Characterisation of an Indonesian very virulent strain of infectious bursal disease virus

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