Nucleotide sequence of ovine ϰ-casein cDNA

Furet, Jean-Pierre; Mercier, Jean‐Claude; Soulier, Solange; Gaye, Pierre; Hue-Delahaie, Dominique; Vilotte, Jean–Luc

doi:10.1093/nar/18.17.5286

Cited by 23 publications

(9 citation statements)

References 8 publications

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“…The sequence surrounding this ATG matched the Kozak (1991) consensus sequence. This number of potential phosphorylation sites is within the range described in different species: three in the mouse, rat (Thompson et al 1985) and pig (Levine et al 1992), four in the ewe (Furet et al 1990) and eight in the guinea-pig (Hall, 1990). This first ATG was thus presumed to be the initiation codon.…”

Section: Resultssupporting

confidence: 51%

“…When prolactin was introduced into the culture medium for the last 3 days, K-casein mRNAs accumulated markedly. In order to determine whether they corresponded to the full-length transcript, the 5' end of their sequences was compared with se¬ quences already published for K-casein cDNA in other species (Stewart et al 1984;Thompson et al 1985;Furet et al 1990;Hall, 1990;Levine et al 1992). 4a).…”

Section: Resultsmentioning

confidence: 99%

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Characterization of rabbit κ-casein cDNA: control of κ-casein gene expression in vivo and in vitro

Bösze

Devinoy

Puissant

et al. 1993

Journal of Molecular Endocrinology

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The rabbit kappa-casein cDNA was cloned and sequenced. One of the isolated clones included almost the entire 5' end, while another clone corresponded to the 3' end of the cDNA. No polyadenylation site was found and therefore this clone did not harbour the complete cDNA. The amino acid sequence of a full-length protein was deduced from the nucleotide sequence obtained for this partial cDNA. It revealed the presence of a chymosin cleavage site and five potential phosphorylation sites. Rabbit kappa-casein was compared with those already described in other species. The rabbit sequence is closer to the ovine than to the mouse sequence. This result supports the idea that Lagomorpha are not closer to Rodentia than to Artiodactyla. The cDNA described above was used to study kappa-casein gene expression in the rabbit mammary gland. This expression was induced primarily by prolactin in mammary gland organoids and was similar to alpha s1-casein gene expression in vivo. The kappa-casein gene present in the casein gene locus is thus subject to the same regulation as the alpha s1-casein gene, although it has evolved from a fibrinogen gene.

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Section: Resultssupporting

confidence: 51%

Section: Resultsmentioning

confidence: 99%

Characterization of rabbit κ-casein cDNA: control of κ-casein gene expression in vivo and in vitro

Bösze

Devinoy

Puissant

et al. 1993

Journal of Molecular Endocrinology

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“…The order Artiodactyla diverged about 64-68 million years ago, and the subfamily Bovinae and the subfamily Caprinae diverged about 20 million years ago (Novacek 1982). The published sequence of the sheep n-casein cDNA (Furet et al 1990) was 92.7% identical with the bovine sequences in the amino acid coding region. The sequence of the water buffalo K-casein gene, which was determined by the same procedure as described in this paper, was 96.7% identical with the bovine sequences (Chikuni et al 1994b).…”

Section: Direct Sequencing For K-casein Genesmentioning

confidence: 85%

“…2. The gene sequences of the sheep and goat were identical to the cDNA sequences (Furet et al 1990;Coll et al 1993) except for a synonymous substitution at position 78 of the sheep gene and a synonymous polymorphism at position 84 of the goat gene. The pig gene differed slightly from the cDNA sequence (Levine et al 1992) at positions 114 and 222.…”

Section: Direct Sequencing For K-casein Genesmentioning

confidence: 99%

“…and pig and exon 3 of the pig h a v e not been sequenced because these regions were not a m p l i f i e d by the PCR. *The nucleotide sequences for the bovine, water buffalo, sheep, goat, and pig K-casein precursors were reported in the p r e v i o u s papers(Alexander et al 1988;C h i k u n i et aL 1994b;Furet et al 1990;Coll et al 1993; L e v i n e et al1992). P o l y m o r p h i c sites and the different nucleotides from the published sequences are underlined.…”

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confidence: 99%

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Molecular phylogeny based on the ?-casein and cytochrome b sequences in the mammalian suborder Ruminantia

Chikuni

Mori²,

Tabata

et al. 1995

J Mol Evol

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Nucleotide sequences for the kappa-casein precursor proteins have been determined from the genomic DNAs or hair roots of the Ruminantia. The coding regions, exons 2, 3, and 4, were amplified separately via the three kinds of PCRs and then directly sequenced. The primers were designed from the sequence of bovine kappa-casein gene; they were applicable for the amplification of the kappa-casein genes from the 13 species in the Ruminantia except exon 2 of the lesser mouse deer. These results permitted an easy phylogenetic analysis based on the sequences of an autosomal gene. A phylogenetic tree was constructed from the mature kappa-casein sequences and compared with the tree of the cytochrome b genes which were sequenced from the same individuals. The Cervidae (sika deer, Cervus nippon) were separated from the branch of the Bovidae on the tree of kappa-casein genes with a relatively high confidence level of the bootstrap analysis, but included in the branch of the Bovidae on the tree of cytochrome b genes. The kappa-casein tree indicated a monophyly of the subfamily Caprinae, although the internal branches were uncertain in the Caprinae. The tree based on the nucleotide sequences of cytochrome b genes clearly showed the relationships of the closely related species in the genus Capricornis consisting of serow (C. smatorensis), Japanese serow (C. crispus), and Formosan serow (C. swinhoei). These results would be explained by the difference of resolving power between the kappa-casein and the cytochrome b sequences.

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Investigating the genetic polymorphism of sheep milk proteins: a useful tool for dairy production

et al. 2014

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Sheep is the second most important dairy species after cow worldwide, and especially in the Mediterranean and Middle East regions. In some countries, the difficult environmental conditions require a peculiar adaptation and, in these contexts, sheep are able to provide higher quality protein than cattle. In the least-developed countries, the amount of dairy sheep and ovine milk production is progressively increasing. In order to improve dairy productions, in particular those with local connotations, it is necessary to obtain in-depth information regarding milk quality and rheological properties. The genetic polymorphisms of milk proteins are often associated with quantitative and qualitative parameters in milk and are potential candidate markers that should be included in breeding strategies similar to those already available for cattle. Due to the current and growing interest in this topic and considering the large amount of new information, the aim of this study was to review the literature on sheep milk protein polymorphisms with a particular emphasis on recent findings in order to give scientists useful support. Moreover, the effects of different protein variants on milk yield and composition are discussed.

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Nucleotide sequence of ovine ϰ-casein cDNA

Cited by 23 publications

References 8 publications

Characterization of rabbit κ-casein cDNA: control of κ-casein gene expression in vivo and in vitro

Characterization of rabbit κ-casein cDNA: control of κ-casein gene expression in vivo and in vitro

Molecular phylogeny based on the ?-casein and cytochrome b sequences in the mammalian suborder Ruminantia

Investigating the genetic polymorphism of sheep milk proteins: a useful tool for dairy production

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