Nucleotide sequence of polypyrimidines from cloned mouse DNA as determined by base-specific blockage of exonuclease action

Deugau, K. V.; Mitchel, R. E. J.; Birnboim, H.C.

doi:10.1016/0003-2697(83)90056-8

Cited by 8 publications

(4 citation statements)

References 20 publications

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“…Plasmid Mm 31 representing the B1 repeat family of the mouse genome (23) was provided by Dr. P. Fort. Plasmids pMR 142 and pMR 288, representing the B2 repetition (24,25) and the "evolutionarily conserved" (EC) sequence (24,26), respectively, were a kind gift of Dr. N. Hastie. A representative of the R group of sequences (27), R1/R2, was given to us by Dr H. Zachau.…”

Section: Methodsmentioning

confidence: 99%

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Molecular cloning and characterization of small polydisperse circular DNA from mouse 3T6 cells

et al. 1986

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We have isolated, cloned and analyzed small polydisperse circular (spc) DNA from mouse 3T6 cells. The representation of highly repeated mouse genome sequence families in spcDNA has been examined, and the B1 repeat appears overrepresented in spcDNA by two criteria. The majority of spcDNA clones, however, is made out by as yet uncharacterized middle repetitive sequences. We have investigated the increase in the spcDNA population upon cycloheximide treatment of individual sequences, which are found to amplify differentially.

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Section: Methodsmentioning

confidence: 99%

“…The only significant deviation from the expected number of colonies is for B1, which apparently is overrepresented about 5-fold. The EC prototype sequence (26) contain no sites for the enzymes used. Apparently cloning in flanking sites was sufficient to permit representation of this element near the expected frequency.…”

Section: -10%mentioning

confidence: 99%

Molecular cloning and characterization of small polydisperse circular DNA from mouse 3T6 cells

et al. 1986

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“…FAS3C lies between FAS3B and FAS8 (Fig. 6) Polypyrimidine tracts are common in the genomic DNA of vertebrates (Deugau et aL, 1983) (Fig. 8).…”

Section: Regulation Of Fas In the Uropygial Glandmentioning

confidence: 99%

Structure and regulation of the avian gene for fatty acid synthase

Goodridge¹,

Carpenter²,

Goldman³

et al. 1989

Reprod. Nutr. Dévelop.

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“…Especially the simple sequence GT/CA has attracted some interest, because it is an alternating purine/pyrimidine sequence which has the potential to form Z-DNA (20,22,23). But also polypyrimidine sequences, which may be simple sequences (24) have been shown to be interspersed repetitive components of eukaryotic genomes (25). We therefore undertook a systematical search for simple sequences in eukaryotes.…”

Section: Introductionmentioning

confidence: 99%

Simple sequences are ubiquitous repetitive components of eukaryotic genomes

1984

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Simple sequences are stretches of DNA which consist of only one, or a few tandemly repeated nucleotides, for example poly (dA) X poly (dT) or poly (dG-dT) X poly (dC-dA). These two types of simple sequence have been shown to be repetitive and interspersed in many eukaryotic genomes. Several other types have been found by sequencing eukaryotic DNA. In this report we have undertaken a systematical survey for simple sequences. We hybridized synthetical simple sequence DNA to genome blots of phylogenetically different organisms. We found that many, probably even all possible types of simple sequence are repetitive components of eukaryotic genomes. We propose therefore that they arise by common mechanisms namely slippage replication and unequal crossover and that they might have no general function with regards to gene expression. This latter inference is supported by the fact that we have detected simple sequences only in the metabolically inactive micronucleus of the protozoan Stylonychia, but not in the metabolically active macronucleus which is derived from the micronucleus by chromosome diminution.

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Nucleotide sequence of polypyrimidines from cloned mouse DNA as determined by base-specific blockage of exonuclease action

Cited by 8 publications

References 20 publications

Molecular cloning and characterization of small polydisperse circular DNA from mouse 3T6 cells

Molecular cloning and characterization of small polydisperse circular DNA from mouse 3T6 cells

Structure and regulation of the avian gene for fatty acid synthase

Simple sequences are ubiquitous repetitive components of eukaryotic genomes

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