Nucleotide sequence of the cDNA encoding silk gland elongation factor 1α

Kamiie, Katsuyoshi; Taira, Hideharu; Ooura, H; Kakuta, Asako; Matsumoto, Shogo; Ejiri, Shin-ichiro; Katsumata, Teizo

doi:10.1093/nar/21.3.742

Cited by 20 publications

(18 citation statements)

References 12 publications

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“…Sequences of COI and COII were aligned against sequences from Dro- 266 sophila yakuba (Clary and Wolstenholme, 1985). Sequences of EF-1␣ were aligned against one of Bombyx mori (Kamiie et al, 1993). Alignments were automated using Sequencher 3.0.…”

Section: Methodsmentioning

confidence: 99%

Phylogeny of Bicyclus (Lepidoptera: Nymphalidae) Inferred from COI, COII, and EF-1α Gene Sequences

Monteiro

Pierce

2001

Molecular Phylogenetics and Evolution

198

125

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Section: Methodsmentioning

confidence: 99%

Phylogeny of Bicyclus (Lepidoptera: Nymphalidae) Inferred from COI, COII, and EF-1α Gene Sequences

Monteiro

Pierce

2001

Molecular Phylogenetics and Evolution

198

125

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“…1 C). Elongation factor In (positions 841 -1150; Kamiie et al, 1993) was also used as a probe. Hybridization and stripping of blots were performed according to the manufacturer's manual (Amersham).…”

Section: Methodsmentioning

confidence: 99%

Tissue‐Specific and Stage‐Specific Expression of Two Silkworm Ecdysone Receptor Isoforms

Kamimura

Tomita

Kiuchi

et al. 1997

European Journal of Biochemistry

105

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Previously, we isolated a cDNA clone for the ecdysone receptor B1 isoform of the silkworm, Bombyx mori (BmEcR-Bl). Here we report the cloning of a cDNA that encodes the Bombyx ecdysone receptor A isoform (BmEcR-A) and mRNA expression of the two BmEcR isoforms during molting and metamorphosis. At larval-pupal transformation, mRNA expression of BmEcR-B 1 was predominant in most tissues examined, including three larval tissues (midgut, epidermis, and fat body) and the wing imaginal disc. The anterior silk gland was the only tissue where BmEcR-A was predominant. These expression patterns were different from observations demonstrated in Drosophila. In the anterior silk gland, both EcR isoforms were expressed synchronously during the fifth larval instar, while expression of the A isoform preceded that of the B1 isoform by two days in the fourth instar. Precedence of BmEcR-A during the fourth instar and synchronization of both isoforms during the fifth instar were also observed in the middle and posterior silk glands, suggesting that transcription of BmEcR in the silk gland is regulated differently in these two instars. In the cultured anterior silk glands of day 0 of the fifth instar, transcription of BmEcR-A and BmEcR-B1 was induced dose dependently by more than 5 ng/ml 20-hydroxyecdysone. BmEcR-A and BmEcR-B1 mRNAs were induced within 2 h and 1 h, respectively, of the addition of 20-hydroxyecdysone. These results suggest that the increase of BmEcR mRNAs during the fifth instar is induced in vivo by a small increase in ecdysteroids.Keywords: ecdysone receptor; Bombyx mori; ecdysteroid ; silk gland ; isoform.Steroid hormones coordinate a wide array of developmental and physiological processes in higher organisms, through binding with receptor proteins to regulate the stage-specific and tissue-specific transcription of target genes. In insects, ecdysteroids, particularly 20-hydroxyecdysone, are the key steroid hormones. 20-Hydroxyecdysone plays a central role in the orchestration of development during molts and metamorphosis. Ashburner et al. (1974) proposed a model for 20-hydroxyecdysone action based on puffing responses of the polytene chromosomes in the Drosophila salivary gland. In this model, 20-hydroxyecdysone binds with an ecdysone receptor protein and directly activates the transcription of a small set of early genes, which then activate many late genes. Recent molecular analyses characterized the ecdysone receptor gene and some of the early genes, most of which are putative transcriptional factors as predicted by Ashburner's model (reviewed in Henrich and Brown, 1995;Thummel, 1995). The ecdysone receptor (EcR) identified from Drosophila is a member of the nuclear receptor superfamily and has three isoforms (A, B1, and B2) with common DNA and

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“…Relative expression is based on the means of WT (+20E) as 100. a For +20E, discs were cultured with 2 µg/ml 20E for 12 h. fected by ecdysteroid titer in wing discs during spinning stage (Quan et al 1998), although we could not detect the increase in β-tubulin mRNA by 20E in cultured wing discs. On the other hand, the mRNA of elongation factor-lα (EF-lα; Kamiie et al 1993) was less abundant in fl wing discs than in WT discs.…”

Section: Non-ecdysteroid-regulated Genesmentioning

confidence: 99%

Expression of ecdysteroid-regulated genes is reduced specifically in the wing discs of the wing-deficient mutant (fl) of Bombyx mori

Matsuoka

Fujiwara

2000

Dev Gene Evol

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The wing-deficient mutant, flügellos (fl), of the silkworm, lacks four wings in the pupa and the adult. Previous studies have suggested that the fl wing discs lose responsiveness to ecdysteroid during metamorphosis. To test this hypothesis at the molecular level we compared the expression of 12 genes when the wing discs from the wild-type (WT) and fl larvae were cultured in the presence or absence of 20-hydroxyecdysone (20E). Most of the genes tested here, ecdysteroid-inducible (EcR-A, -B1, and E75) and noninducible genes (actin A3, beta-tubulin, apterous (ap), USP, and BHR38) were normally expressed in the fl wing discs. However, the amounts of mRNAs of two ecdysteroid-inducible genes, BHR3 (early-late gene; Bombyx homologue to DHR3 and MHR3) and Urbain (wing-specific late gene), were reduced to about 50% and 20% of WT in the cultured fl wing discs, respectively. We analyzed developmental profiles of these mRNAs during metamorphosis. They also demonstrated decreased BHR3 and Urbain mRNA 2 days after the onset of wandering. This reduction in transcription of BHR3 in the fl mutant was observed only in the wing disc, not in the testis and fatbody. These results imply that the aberrant expression of the fl gene affects the downstream pathway of ecdysteroid signaling specifically in the wing discs and thus leads to a deficiency in wing formation.

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Nucleotide sequence of the cDNA encoding silk gland elongation factor 1α

Cited by 20 publications

References 12 publications

Phylogeny of Bicyclus (Lepidoptera: Nymphalidae) Inferred from COI, COII, and EF-1α Gene Sequences

Phylogeny of Bicyclus (Lepidoptera: Nymphalidae) Inferred from COI, COII, and EF-1α Gene Sequences

Tissue‐Specific and Stage‐Specific Expression of Two Silkworm Ecdysone Receptor Isoforms

Expression of ecdysteroid-regulated genes is reduced specifically in the wing discs of the wing-deficient mutant (fl) of Bombyx mori

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