Nucleotide sequence of the coat protein gene of a strain of clover yellow vein virus from New Zealand: conservation of a stem-loop structure in the 3′ region of potyviruses

Bryan, Gregory T.; Gardner, Richard C.; Forster, R. L. S.

doi:10.1007/bf01314631

Cited by 26 publications

(17 citation statements)

References 51 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Elle se termine par la queue poly(A) codée par le génome viral (Dolja et Carrington, 1992). Cette région 3'NTR est impliquée dans l'initiation de la synthèse du brin négatif d'ARN (Bryan et al, 1992 ;Dolja et Carrington, 1992), ainsi que dans la sévérité de l'expression des symptômes (Rodriguez-Cerezo et al, 1991 (Frenkel et al, 1989). Les Le rôle des structures «tige-boucle» de la région 3'NTR a été démontré dans les interactions spécifiques entre l'ARN viral et la protéine capsidiaire chez le phage R17 (Carey et al, 1983), dans la transactivation de la protéine «tat» chez le virus de l'immunodéficience humaine (HIV) (Feng et Holland, 1988), et dans l'induction des symptômes caractéristiques de l'infection virale chez le TVMV (Rodriguez-Cerezo et al, 1991).…”

Section: Introductionunclassified

Polymorphisme des régions capside et 3'NTR de 3 isolats tunisiens du virus Y de la pomme de terre (PVY)

Fakhfakh¹,

Makni²,

Robaglia

et al. 1995

Agronomie

View full text Add to dashboard Cite

Section: Introductionunclassified

Polymorphisme des régions capside et 3'NTR de 3 isolats tunisiens du virus Y de la pomme de terre (PVY)

Fakhfakh¹,

Makni²,

Robaglia

et al. 1995

Agronomie

View full text Add to dashboard Cite

“…Comparison of coat protein sequences also showed that the amino terminal region of that of C1YVV [5,44,45] spanning amino acid residues 18 to 30 (Fig. 4) does not show any sequence identity with the corresponding coat protein region of strains of BYMV whose sequences have been determined to date [-6, 17, 43, 44], suggesting that the epitopes of the C1YVV-specific MAbs 1, 2, 4, and 13 are located in this region.…”

Section: Discussionmentioning

confidence: 95%

“…A comparison of coat protein amino acid sequences from the surface-exposed, amino terminal region of strains of BYMV and C1YVV [5,6,17,[43][44][45] shows that the coat proteins of these two viruses have 11 of the first 17 amino-terminal amino acid residues identical (Fig. 4).…”

Section: Discussionmentioning

confidence: 99%

“…Therefore, some workers considered BYMV, C1YVV, and PMV to be strains of the same virus [21], whereas others considered them to be different viruses [3,28]. Nucleotide sequences of the coat protein gene and the 3' noncoding regions of RNA from four strains of BYMV [6,17,43,44] and three strains of C1YVV [5,44,45] have recently been determined. The sequences fell into distinct clusters indicating that BYMV and C1YVV are distinct potyviruses [44].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Characterisation and epitope analysis of monoclonal antibodies to virions of clover yellow vein and Johnsongrass mosaic potyviruses

Hewish

Xiao

Mishra

et al. 1993

Archives of Virology

View full text Add to dashboard Cite

Mouse monoclonal antibodies (MAbs) against the Australian B strain of clover yellow vein (ClYVV-B) and the JG strain of Johnsongrass mosaic (JGMV) potyviruses were produced, characterised and the epitopes with which they reacted were deduced. Using intact particles of ClYVV a total of ten MAbs were obtained which reacted strongly with ClYVV-B in an enzyme-linked immunosorbent assay and Western blots. Four of these MAbs (1, 2, 4, and 13) were found to be ClYVV-specific, as they reacted with all five ClYVV strains from Australia and the U.S.A. but not with 11 strains of bean yellow mosaic (BYMV), pea mosaic (PMV), and white lupin mosaic (WLMV) viruses which, together with ClYVV, form the BYMV subgroup of potyvirses. These MAbs failed to react with eight other potyvirus species, including six which infect legumes like the viruses in the BYMV subgroup. The ClYVV MAb 10 was found to be BYMV subgroup-specific. It reacted strongly with 15 of the 16 strains of viruses in the subgroup and gave no reaction with eight other potyviruses. The other five ClYVV MAbs reacted with varying degrees of specificity with the BYMV subgroup viruses and also with other potyviruses. Eight of the ClYVV MAbs (1, 2, 4, 5, 13, 17, 21, and 22) reacted with the intact coat proteins only and not with the truncated (minus amino terminus) coat protein of ClYVV suggesting that the epitopes for these MAbs are located in the surface-exposed, amino-terminal region of the ClYVV coat protein. Comparison of published coat protein sequences of BYMV and ClYVV isolates indicated that the epitopes for the four ClYVV-specific MAbs may be in the amino-terminal region spanning amino acid residues 18 to 30, whereas those for the other four MAbs may be located in the first 17 amino-terminal amino acid residue region. The epitopes that reacted with BYMV subgroup-specific MAb 10 and MAb 30 which reacted with 20 of the 24 potyvirus isolates, are probably located in the core region of ClYVV coat protein as these MAbs reacted with the intact as well as truncated coat protein of ClYVV. Analysis, in Western blot immunoassay, of 17 MAbs raised against virions of JGMV revealed that only two MAbs (1-25 and 4-30) were JGMV-specific, whereas others displayed varying degrees of specificity to different potyviruses.(ABSTRACT TRUNCATED AT 400 WORDS)

show abstract

“…3). The 3% non-coding region of both viruses contains the consensus sequences GAGG (position 1029-1032 and 1032-1035 in SPLV-CH and SPLV-T, respectively) and CCTC (position 1038-1041 and 1041-1044 in SPLV-CH and SPLV-T, respectively) separated by five nucleotides, which could form a stem-loop structure possibly having a role in replication (Bryan et al, 1992). Nucleotide sequence identity between SPLV-CH and -T in the 3% non-coding region was 97.0%.…”

Section: Sequence Analysis Of Splv-ch and -T Pcr Productsmentioning

confidence: 99%

Evidence for the assignment of two strains of SPLV to the genus Potyvirus based on coat protein and 3′ non-coding region sequence data

1997

View full text Add to dashboard Cite

The use of potyvirus-specific primers and subsequent application of the RACE procedure allowed the cloning of the 3% terminal 1088 nucleotides of the genomic RNA of the Taiwan isolate of sweetpotato latent virus (SPLV-T) and the 3% genomic 1085 nucleotides of a SPLV-like virus from China (SPLV-CH). The sequence of an internal part of the presumptive nuclear inclusion b gene was also determined for both isolates. Detailed sequence analyses revealed the presence of consensus motifs which indicated that SPLV-CH and SPLV-T should be regarded as members of the genus Poty6irus. Multiple sequence alignments and phylogenetic analyses were also performed and unambiguously assessed these isolates as strains of a distinct Poty6irus. SPLV was not related to other potyviruses infecting sweetpotato nor to any other sequenced virus. From the presence of the DAG box, SPLV-CH is expected to be a typical aphid transmitted Poty6irus whereas a conceivable explanation is proposed for the non-aphid transmission of SPLV-T. © 1997 Elsevier Science B.V.

show abstract

Nucleotide sequence of the coat protein gene of a strain of clover yellow vein virus from New Zealand: conservation of a stem-loop structure in the 3′ region of potyviruses

Cited by 26 publications

References 51 publications

Polymorphisme des régions capside et 3'NTR de 3 isolats tunisiens du virus Y de la pomme de terre (PVY)

Polymorphisme des régions capside et 3'NTR de 3 isolats tunisiens du virus Y de la pomme de terre (PVY)

Characterisation and epitope analysis of monoclonal antibodies to virions of clover yellow vein and Johnsongrass mosaic potyviruses

Evidence for the assignment of two strains of SPLV to the genus Potyvirus based on coat protein and 3′ non-coding region sequence data

Contact Info

Product

Resources

About