1991
DOI: 10.1128/jb.173.1.101-107.1991
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Nucleotide sequence of the glpD gene encoding aerobic sn-glycerol 3-phosphate dehydrogenase of Escherichia coli K-12

Abstract: Aerobic sn-glycerol 3-phosphate dehydrogenase, encoded by the glpD gene of Escherichia coli, is a cytoplasmic membrane-associated respiratory enzyme. The nucleotide sequence of glpD was determined. An open reading frame of 501 codons was preceded by a consensus Shine-Dalgarno sequence. The proposed translational start and reading frame of glpD were confirmed by determining the nucleotide sequence across the fusion joint of a glpD-lacZ translational fusion. The predicted molecular weight, 56,750, corresponds we… Show more

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Cited by 62 publications
(34 citation statements)
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“…GylC shares 49% amino acid identity to GlpF of B. subtilis (5). GylA and GylB exhibit 52 and 32% identity to glycerol kinase GlpK and glycerol-3-phosphate dehydrogenase GlpD of E. coli, respectively (4,26). GylX shares similarity with proteins of unknown function present in other actinomycetes and archaea.…”
Section: Resultsmentioning
confidence: 93%
“…GylC shares 49% amino acid identity to GlpF of B. subtilis (5). GylA and GylB exhibit 52 and 32% identity to glycerol kinase GlpK and glycerol-3-phosphate dehydrogenase GlpD of E. coli, respectively (4,26). GylX shares similarity with proteins of unknown function present in other actinomycetes and archaea.…”
Section: Resultsmentioning
confidence: 93%
“…Stringent two-tiered regulation of this pathway is by means of both transcriptional activation and membrane localization, which ensures GlpD is fully active only when it is associated with the cytoplasmic membrane or when reconstituted with phospholipids or amphiphiles in vitro. These mechanisms ensure that cellular energy needs are met while lipid biosynthesis is not disrupted (1)(2)(3)(4)(5)11). To structurally characterize this important enzyme, we have determined the structures of GlpD in its native form and cocrystallized with substrate analogues, product, and UQ analogues (Tables 1 and 2).…”
mentioning
confidence: 99%
“…The membraneassociated glycerol-3-phosphate dehydrogenase (GlpD) is one of the key flavin-linked primary dehydrogenases of the respiratory electron transport chain and serves an essential function for aerobic growth on glycerol (1)(2)(3). GlpD catalyzes the oxidation of G3P to dihydroxyacetone phosphate (DHAP), with concurrent reduction of flavin adenine dinucleotide (FAD) to FADH 2 , and passes electrons on to ubiquinone (UQ) and ultimately to oxygen or nitrate (Fig.…”
mentioning
confidence: 99%
“…The genes of this regulon are organized in at least five operons which map in three separate regions on the chromosome (13). The glpFK operon encodes a membrane diffusion facilitator for glycerol and a cytoplasmic glycerol kinase (37), the glpTQ operon encodes a membrane-associated G3P permease and a periplasmic glycerophosphodiester phosphodiesterase (8,34), the glpACB operon (4) encodes anaerobic G3P dehydrogenase, the glpD gene encodes aerobic G3P dehydrogenase (1), and glpEG encodes proteins with unknown functions (22). By genetic (5) and physical means (21,22,37), it has conclusively been demonstrated that each operon is negatively regulated by a single repressor encoded by glpR, which maps adjacent to and is divergently transcribed from glpD (21,22).…”
mentioning
confidence: 99%