Nucleotide sequence of the<i>gyrA</i>gene of<i>Arcobacter</i>species and characterization of human ciprofloxacin-resistant clinical isolates

Abdelbaqi, Khalil; Ménard, Armelle; Prouzet‐Mauléon, Valérie; Bringaud, Frédéric; Lehours, Philippe; Mégraud, Françis

doi:10.1111/j.1574-695x.2006.00208.x

Cited by 67 publications

(53 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…For PCR amplification of the gyrA gene (encoding subunit A of DNA gyrase), genomic DNA was extracted from strain CL-S1 T and A. halophilus DSM 18005 T (Donachie et al, 2005) by using the AccuPrep genomic DNA extraction kit. To amplify the gyrA gene, we first employed PCR by using two sets of degenerate primers (Abdelbaqi et al, 2007). However, no PCR product was obtained from the genomic DNA of either strain CL-S1 T or A. halophilus DSM 18005 T in our study (data not shown).…”

mentioning

confidence: 85%

Arcobacter marinus sp. nov.

Kim

Hwang

Cho

2010

International Journal of Systematic and Evolutionary Microbiology

View full text Add to dashboard Cite

A slightly curved, rod-shaped marine bacterium, designated strain CL-S1 T , was isolated from near Dokdo, an island in the East Sea, Korea. Cells were Gram-negative and grew well under either aerobic or microaerobic conditions. Analyses of the 16S rRNA and gyrA gene sequences of strain CL-S1 T revealed an affiliation with the genus Arcobacter within the class Epsilonproteobacteria. Phylogenetic analyses based on 16S rRNA and gyrA gene sequences showed that strain CL-S1 T formed a robust clade with Arcobacter halophilus LA31B T , with sequence similarities of 96.1 and 88.2 %, respectively. DNA-DNA relatedness between strain CL-S1 T and A. halophilus DSM 18005 T was 44 %, indicating that they represent genomically distinct species. Strain CL-S1 T grew optimally at 30-37 6C, at pH 7 and in the presence of 3-5 % NaCl. The dominant cellular fatty acids were iso-C 15 : 0 2-OH and/or C 16 : 1 v7c (28.4 %), C 16 : 0 (26.2 %) and C 18 : 1 v7c (22.3 %). The DNA G+C content of strain CL-S1 T was 28 mol%. Strain CL-S1 T differed phenotypically from A. halophilus LA31B T based on its ability to grow aerobically at 10 6C and inability to grow under anaerobic conditions. Based on the data presented, strain CL-S1 T is considered to represent a novel species of the genus Arcobacter, for which the name Arcobacter marinus sp. nov. is proposed. The type strain is CL-S1 T (5KCCM 90072 T 5JCM 15502 T ).The genus Arcobacter belongs to the family Campylobacteraceae, which also contains the genera Campylobacter and Sulfurospirillum ( (Neill et al., 1985;Kiehlbauch et al., 1991;Vandamme et al., 1992) and are recognized as potential emerging human pathogens (Mansfield & Forsythe, 2000). A. nitrofigilis was isolated from Spartina alterniflora roots in a salt marsh (McClung et al., 1983). A. halophilus and A. cibarius were isolated from a hypersaline lagoon (Donachie et al., 2005) and the skin of a broiler carcass (Houf et al., 2005), respectively. In the present study, a novel strain affiliated with the genus Arcobacter was isolated and subjected to a polyphasic taxonomic analysis.Seaweeds and a starfish were collected and added to a surface seawater sample (50 ml) taken from the vicinity of Dokdo, an island in the East Sea, Korea. The mixture was maintained at 4 u C for 15 days. The mixture was then homogenized by using a blender and an aliquot (50 ml) was spread onto MY medium (Bouchotroch et al., 2001). The culture was incubated aerobically at 30 u C for 2 weeks. Strain CL-S1 T was isolated and subsequently purified on fresh marine agar 2216 (MA; Difco). Strain CL-S1 T grew well on MA or on saline blood agar [SBA; per litre distilled water: 40 g blood agar base (BBL), 50 ml sheep blood, 30 g NaCl] at 37 u C under either aerobic or microaerobic conditions. The novel strain was preserved in marine broth 2216 (MB; Difco) supplemented with 30 % (v/v) glycerol at 280 u C.For 16S rRNA gene amplification by PCR, DNA was extracted from a single colony by a boiling method (Englen & Kelley, 2000). The crude extracts served as the DNA template for...

show abstract

mentioning

confidence: 85%

Arcobacter marinus sp. nov.

Kim

Hwang

Cho

2010

International Journal of Systematic and Evolutionary Microbiology

View full text Add to dashboard Cite

show abstract

“…30°C, 24-72 h, mO 2 plating medium. This is the only method that has been validated for fecal specimens, by evaluating the recovery of arcobacters from artificially contaminated fecal samples (76).…”

Section: Isolation and Detectionmentioning

confidence: 99%

Taxonomy, Epidemiology, and Clinical Relevance of the Genus Arcobacter

2011

View full text Add to dashboard Cite

SUMMARY The genus Arcobacter , defined almost 20 years ago from members of the genus Campylobacter , has become increasingly important because its members are being considered emergent enteropathogens and/or potential zoonotic agents. Over recent years information that is relevant for microbiologists, especially those working in the medical and veterinary fields and in the food safety sector, has accumulated. Recently, the genus has been enlarged with several new species. The complete genomes of Arcobacter butzleri and Arcobacter nitrofigilis are available, with the former revealing diverse pathways characteristic of free-living microbes and virulence genes homologous to those of Campylobacter. The first multilocus sequence typing analysis showed a great diversity of sequence types, with no association with specific hosts or geographical regions. Advances in detection and identification techniques, mostly based on molecular methods, have been made. These microbes have been associated with water outbreaks and with indicators of fecal pollution, with food products and water as the suspected routes of transmission. This review updates this knowledge and provides the most recent data on the taxonomy, species diversity, methods of detection, and identification of these microbes as well as on their virulence potential and implication in human and animal diseases.

show abstract

“…PCR, multiplex PCR, real-time PCR, and multiplex real-time PCR for detecting Arcobacter species were developed, targeting the 16S or 23S rRNA, rpoB-rpoC, and gyrA genes (24)(25)(26). Loop-mediated isothermal amplification (LAMP) was recently developed for onthe-spot inspections (27).…”

mentioning

confidence: 99%

Comparison of Conventional PCR, Multiplex PCR, and Loop-Mediated Isothermal Amplification Assays for Rapid Detection of Arcobacter Species

et al. 2014

View full text Add to dashboard Cite

This study aimed to develop a loop-mediated isothermal amplification (LAMP) method for the rapid detection of Arcobacter species. Specific primers targeting the 23S ribosomal RNA gene were used to detect Arcobacter butzleri, Arcobacter cryaerophilus, and Arcobacter skirrowii. The specificity of the LAMP primer set was assessed using DNA samples from a panel of Arcobacter and Campylobacter species, and the sensitivity was determined using serial dilutions of Arcobacter species cultures. LAMP showed a 10-to 1,000-fold-higher sensitivity than multiplex PCR, with a detection limit of 2 to 20 CFU per reaction in vitro. Whereas multiplex PCR showed cross-reactivity with Campylobacter species, the LAMP method developed in this study was more sensitive and reliable than conventional PCR or multiplex PCR for the detection of Arcobacter species.

show abstract

Nucleotide sequence of thegyrAgene ofArcobacterspecies and characterization of human ciprofloxacin-resistant clinical isolates

Cited by 67 publications

References 27 publications

Arcobacter marinus sp. nov.

Arcobacter marinus sp. nov.

Taxonomy, Epidemiology, and Clinical Relevance of the Genus Arcobacter

Comparison of Conventional PCR, Multiplex PCR, and Loop-Mediated Isothermal Amplification Assays for Rapid Detection of Arcobacter Species

Contact Info

Product

Resources

About