Nucleotide sequence of the<i>Streptococcus pneumoniae ung</i>gene encoding uracil-DNA glycosylase

Méjean, Vincent; Rives, I; CIaverys, J.-P.

doi:10.1093/nar/18.22.6693

Cited by 20 publications

(8 citation statements)

References 6 publications

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“…4). In the case of the ung gene (Méjean et al, 1990), the BPROM prediction program (Softberry, Inc.) revealed a consensus -10 hexamer, which is located 28 nucleotides upstream of the translation initiation codon, and a near-consensus -10 extension (Fig. 4).…”

Section: Use Of Plasmid Past As a Promoter-probe Vector In S Pneumonmentioning

confidence: 99%

Novel plasmid-based genetic tools for the study of promoters and terminators in Streptococcus pneumoniae and Enterococcus faecalis

Ruiz‐Cruz

Solano-Collado

Espinosa

et al. 2010

Journal of Microbiological Methods

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Promoter-probe and terminator-probe plasmid vectors make possible to rapidly examine whether particular sequences function as promoter or terminator signals in various genetic backgrounds and under diverse environmental stimuli. At present, such plasmid-based genetic tools are very scarce in the Gram-positive pathogenic bacteria Streptococcus pneumoniae and Enterococcus faecalis. Hence, we developed novel promoter-probe and terminator-probe vectors based on the Streptococcus agalactiae pMV158 plasmid, which replicates autonomously in numerous Gram-positive bacteria. As reporter gene, a gfp allele encoding a variant of the green fluorescent protein was used. These genetic tools were shown to be suitable to assess the activity of promoters and terminators (both homologous and heterologous) in S. pneumoniae and E. faecalis. In addition, the promoter-probe vector was shown to be a valuable tool for the analysis of regulated promoters in vivo, such as the promoter of the pneumococcal fuculose kinase gene. These new plasmid vectors will be very useful for the experimental verification of predicted promoter and terminator sequences, as well as for the construction of new inducible-expression vectors. Given the promiscuity exhibited by the pMV158 replicon, these vectors could be used in a variety of Gram-positive bacteria.

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Section: Use Of Plasmid Past As a Promoter-probe Vector In S Pneumonmentioning

confidence: 99%

Novel plasmid-based genetic tools for the study of promoters and terminators in Streptococcus pneumoniae and Enterococcus faecalis

Ruiz‐Cruz

Solano-Collado

Espinosa

et al. 2010

Journal of Microbiological Methods

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“…This cDNA encodes the major nuclear form, as well as the mitochondrial form of the enzyme [12,13]. The UNG-protein shows a striking similarity to analogous enzymes from E. coli [14], Streptococcuspneumoniae [15], animal viruses [16][17][18][19] and Saccharomyces cerevisiae [20]. Although all human cells and tissues investigated have measurable UNG activities, significant interindividual as well as interorgan variation in activities has been observed in extracts from human adult tissues [21,22].…”

Section: Introductionmentioning

confidence: 99%

Structure of the gene for human uracil—DNA glycosylase and analysis of the promoter function

Haug¹,

Skorpen²,

Lund³

et al. 1994

FEBS Letters

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The gene for human uracil-DNA glycosylase (UNG) contains 4 exons and has an approximate size of 13 kb. The promoter is very GC rich and lacks a TATA box. Nested deletions of the promoter demonstrated that two SP1 elements and a putative c-MYC element proximal to the transcription initiation region were sufficient to support some 27% of the promoter activity, while a clone that in addition contained the elements E2F/SPI/CCAAT increased expression to almost 90% of the full-length construct. A region upstream of these elements appears to exert a negative control function.

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“…Recently, uracil-DNA glycosylase from human placenta was purified and partially sequenced (23), the corresponding cDNA (UNG15 and UNG40) was cloned (24), and the gene assigned to chromosome 12 (25). A strong sequence similarity was observed to UDG from E. coli (26), Streptococcus pneumoniae (27), animal viruses (28)(29)(30)(31) and yeast (32), ranging from 41 to 56% identical residues. Unexpectedly, the human protein showed highest similarity to the E. coli protein, and this similarity was 73 % when conservative amino acid substitutions were included.…”

Section: Introductionmentioning

confidence: 99%

Cell cycle regulation andin vitrohybrid arrest analysis of the major human uracil-DNA glycosylase

et al. 1991

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Uracil-DNA glycosylase (UDG) is the first enzyme in the excision repair pathway for removal of uracil in DNA. In vitro transcription/translation of a cloned human cDNA encoding UDG resulted in easily measurable UDG activity. The apparent size of the primary translation product was 34 kD. Two lines of evidence indicated that this cDNA encodes the major nuclear UDG. First, in vitro translation of human fibroblast mRNA isolated from S-phase cells resulted in measurable UDG activity and this UDG translation was specifically inhibited 90% by an anti-sense UDG mRNA transcript. Secondly, cell cycle analysis revealed an 8-12 fold increase in transcript level late in the G1-phase preceding a 2-3 fold increase in total UDG activity in the S-phase. UDG degradation was found to be very slow (T1/2 approximately 30h), therefore, the rate of UDG synthesis could be derived from the rate of UDG accumulation, and was found to correlate temporarily and quantitatively with the transcript level. Inhibitor studies showed that RNA and protein synthesis was required for induction of UDG. However, specific inhibition of DNA replication with aphidicolin indicated that entrance of fibroblasts into the S-phase was not required for UDG accumulation.

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Nucleotide sequence of theStreptococcus pneumoniae unggene encoding uracil-DNA glycosylase

Cited by 20 publications

References 6 publications

Novel plasmid-based genetic tools for the study of promoters and terminators in Streptococcus pneumoniae and Enterococcus faecalis

Novel plasmid-based genetic tools for the study of promoters and terminators in Streptococcus pneumoniae and Enterococcus faecalis

Structure of the gene for human uracil—DNA glycosylase and analysis of the promoter function

Cell cycle regulation andin vitrohybrid arrest analysis of the major human uracil-DNA glycosylase

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