Nucleotide sequence of the mouse .alpha.1-acid glycoprotein gene 1

Abstract

In a previous paper we presented evidence for the existence of at least two alpha 1-acid glycoprotein (AGP) genes in the mouse. One of the cDNA clones characterized in those studies was used to isolate several unique AGP genomic clones. In these studies we present the complete sequence of one of the mouse AGP genes. The sequence analysis includes 595 base pairs (bp) 5' to the site of initiation of transcription and 135 bp 3' to the polyadenylation signal. This mouse AGP gene, designated AGP-1, has six exons, a structure similar to those of the AGP genes in rats and humans. Analysis of the sequence has revealed a number of potential regulatory sites. These include a run of alternating purine-pyrimidine bases [(GT)N] at +2890 to +2945, flanked by three potential glucocorticoid receptor binding sites within intron 5. Two of these TGTTCT at +3069 to +3074 and +3082 to +3087 flank the (GT)n track at its 3' end, and one, which is oriented in the opposite direction (AGAACA), at +2771 to +2776 flanks the track at its 5' end. A longer version of the glucocorticoid receptor site, GGGTACAATGTGTCCT, has been located in the 5' flanking region of the gene (-94 to -79); the sequence AGAACA is another potential glucocorticoid receptor site oriented in the opposite direction and located at -127 to -122. This entire region, from -146 to -42, in the mouse has a strong homology (approximately 85%) to the 5' flanking region of the rat AGP gene, which contains a 78-bp fragment (-120 to -42) that represents the minimal sequence required for glucocorticoid regulation.(ABSTRACT TRUNCATED AT 250 WORDS)

Molecular Mechnisms of Liver‐Specific Albumin and α‐Fetoprotein Gene Regulation: A Review

“…The consensus sequences used were C/EBP, TGTGG(AT)(AT)(AT)G (43): HNF-1, GTTAATnATTAAC (3,9): HNF-3, TATTGA(CT)TT(AT)G (15); HNF-4, GGCAAGGTTCAT (15). The genomic sequences used were mouse AFP (92), rat AFP (58,5960), human AFP (73), mouse albumin (40), rat albumin (59), human albumin (86), human and mouse a,-antitrypsin (32), human and mouse transthyretin (1 7), mouse AFP (14), mouse C-reactive protein (63), and mouse serum amyloid A gene 1 to -94 were required for the specific efficient direction of tissue specific transcription in the differentiated hepatoma cell line H411. To improve the efficiency of signal expression, lzban and Papaconstantinou (40) conducted transient expression analyses with a series of expression vectors composed of the Moloney murine leukemia virus enhancer which was linked to a series of deleted mouse albumin promoter segments and to the CAT reporter gene.…”

“…Other liver-specific genes, such a,-acid glycoprotein (AGP) (14), serum amyloid A (SAA) (51), and C-reactive protein (CRP) (63), lack binding sites for these liver-specific factors. AGP, SAA, and CRP are members of a large family of liver-specific genes, the acute phase reactant genes (APR), whose major site of synthesis is the liver, although other cell types such as macrophages, keratinocytes and fibroblasts synthesize these proteins (27).…”

“…The consensus sequences used were C/EBP, TGTGG(AT)(AT)(AT)G (43): HNF-1, GTTAAT-nATTAAC (3,9): HNF-3, TATTGA(CT)TT(AT)G (15); HNF-4, GGCAAGGTTCAT (15). The genomic sequences used were mouse AFP (92), rat AFP (58,5960), human AFP (73), mouse albumin (40), rat albumin (59), human albumin (86), human and mouse a,-antitrypsin (32), human and mouse transthyretin (1 7), mouse AFP (14), mouse C-reactive protein (63), and mouse serum amyloid A gene 1, 2, 3, (51). the albumin gene, Gorski et a/.…”

Evidence for Posttranscriptional Regulation of C/EBPα and C/EBPβ Isoform Expression during the Lipopolysaccharide-Mediated Acute-Phase Response†

“…By using the oligonucleotide corresponding to the C/EBPbinding site of the AGP-1 promoter (APRE), we have demonstrated a major shift in the DNA-binding activities from C/EBP␣ to C/EBP␤ during the response to LPS (2,3,23).…”

Orosomucoid typing of apes (family Pongidae) by isoelectric focusing: Among primates do only humans have two functional orosomucoid loci?

“…The cDNAs corresponding to two types of ORM have been cloned and sequenced (Cooper and Papaconstantinou, 1986;Cooper et al, 1987;Lee et aL, 1989). A wild mouse strain, Mus caroli, which diverged from both inbred mice and rats 0.5-1 x 10 7 years ago, was revealed to have as many as eight distinct ORM genes in the haploid genome (Prowse and Baumann, 1990).…”