Nucleotide sequence of the phosphinothricin N-acetyltransferase gene from Streptomyces viridochromogenes Tü494 and its expression in Nicotiana tabacum

Wohlleben, Wolfgang; Arnold, Walter; Broer, Inge; Hillemann, Doris; Strauch, Eckhard; Pühler, Alfred

doi:10.1016/0378-1119(88)90101-1

Cited by 256 publications

(121 citation statements)

References 31 publications

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“…However, PAT cannot restrain the activity of glutamine synthetase. Thus, PAT can induce transgenic crops to become resistant to herbicide, and the toxicity caused by glufosinate is eliminated [26]. S. hygroscopicus, which are a part of the biosphere, are widespread in nature.…”

Section: Discussionmentioning

confidence: 99%

Effects of bar-transgenic rice on the intestinal microflora of the mice (<i>Mus musculus</i>)

Liu¹,

Huang²,

Sun³

et al. 2012

AJMB

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Microbial molecular ecology approaches were used to the effects of Bar-transgenic rice on Intestinal Microflora of the Mice (Mus musculus). Kunming mice (Mus musculus) of 100 SPF-grade (20 ± 2 g), half of which were male and the other half female, were randomly divided into five groups with four replications per group and five mice per replication to assess the safety of Bar-transgenic rice. Five diets meeting or exceeding the minimum nutrient requirement was fed for 180 days. After 90 days, parental generation (P) was bred to produce the first filial generation (F 1 ). Each generation was fed for 180 days. On the 180 th day, five mice from each group were randomly sampled, and their intestinal contents were collected for DNA isolation. The V3 region of the 16S rDNA was amplified by polymerase chain reaction (PCR) and analyzed via denaturing gradient gel electrophoresis (DGGE). The resulting PCR-DGGE band number (bacterial species) was counted, and the banding patterns were analyzed by calculating the Sorenson's pairwise similarity coefficients (Cs), an index used to measure bacterial species found among all samples. The sequence analysis of bands was performed to identify the intestinal predominant microflora of the mice. The intergroup Cs values of the samples across all groups did not differ (P > 0.05) from each other. The effect of Bar-transgenic rice on the intestinal microflora of the mice was considered insignificant.

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Section: Discussionmentioning

confidence: 99%

Effects of bar-transgenic rice on the intestinal microflora of the mice (<i>Mus musculus</i>)

Liu¹,

Huang²,

Sun³

et al. 2012

AJMB

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“…The gene encoding the acetylating enzyme has been isolated from Streptomyces hygroscopicus (Thompson et al, 1987) and from S. viridochromogenes (Wohlleben et al, 1988). It has been referred to as bar (for bialaphos resistance) and PAT gene, respectively.…”

Section: Mechanisms For Conferring Herbicide Tolerance In Cropsmentioning

confidence: 99%

Herbicide Tolerant Food Legume Crops: Possibilities and Prospects

Singh¹,

Yadav²

2012

Herbicides - Properties, Synthesis and Control of Weeds

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“…Inhibition of the enzyme results in ammonia accumulation and disruption of chloroplast and finally cell death due to photosynthesis inhibition (Lindsey, 1992;OECD, 1999). In plant transformation studies, as herbicide resistance selection marker gene, pat from S. viridochromogenes (Wohlleben et al, 1988) and bar gene from S. hygroscopicus (bialophos resistance; Thompson et al, 1987) encoding the enzyme phosphinothricin N-acetyltransferase (PAT) are extensively used for resistance to PPT. PAT converts PPT to a non-herbicidal acetylated form by transferring the acetyl group from acetyl CoA to the free amino group of PPT (Miki & McHugh, 2004).…”

Section: Selection Systems a Critical Stepmentioning

confidence: 99%

Recent Advances in Fruit Species Transformation

Akdemir¹,

Gago²,

Gallego³

et al. 2012

Transgenic Plants - Advances and Limitations

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Nucleotide sequence of the phosphinothricin N-acetyltransferase gene from Streptomyces viridochromogenes Tü494 and its expression in Nicotiana tabacum

Cited by 256 publications

References 31 publications

Effects of bar-transgenic rice on the intestinal microflora of the mice (<i>Mus musculus</i>)

Effects of bar-transgenic rice on the intestinal microflora of the mice (<i>Mus musculus</i>)

Herbicide Tolerant Food Legume Crops: Possibilities and Prospects

Recent Advances in Fruit Species Transformation

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Nucleotide sequence of the phosphinothricin N-acetyltransferase gene from Streptomyces viridochromogenes Tü494 and its expression in Nicotiana tabacum

Cited by 256 publications

References 31 publications

Effects of bar-transgenic rice on the intestinal microflora of the mice (&lt;i&gt;Mus musculus&lt;/i&gt;)

Effects of bar-transgenic rice on the intestinal microflora of the mice (&lt;i&gt;Mus musculus&lt;/i&gt;)

Herbicide Tolerant Food Legume Crops: Possibilities and Prospects

Recent Advances in Fruit Species Transformation

Contact Info

Product

Resources

About

Effects of bar-transgenic rice on the intestinal microflora of the mice (<i>Mus musculus</i>)

Effects of bar-transgenic rice on the intestinal microflora of the mice (<i>Mus musculus</i>)