Nucleotide sequence of the purM gene encoding 5'-phosphoribosyl-5-aminoimidazole synthetase of Escherichia coli K12.

Smith, John L.; Daum, H A

doi:10.1016/s0021-9258(18)67432-0

Cited by 50 publications

(4 citation statements)

References 35 publications

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“…pJS119. Plasmid pJSl 19 (Figure 1A) was constructed by two successive subclonings of restriction fragments that covered the nucleotide sequence 732-2746 [the numbering scheme refers to the published sequence (Smith & Daum, 1986 and 1987)] which removes the purMN promoter and purR binding site. The first restriction fragment subcloned was a 186-bp HinP\ fragment (nucleotide 732-919) that had been treated with T4 DNA polymerase to create blunt ends and cloned into the Smal site of M13mpl8 (Yanisch-Perron et al, 1985).…”

Section: Construction Of Air Synthase and Gar Tfase Expressionmentioning

confidence: 99%

“…pJS167. This plasmid was created by a series of manipulations equivalent to the deletion of the purM coding region for AIR (Smith & Daum, 1986). During the intermediate steps in the construction of the expression vector, clonings were carried out in such a way as to maintain expression via the lac promoter on the host plasmids.…”

Section: Construction Of Air Synthase and Gar Tfase Expressionmentioning

confidence: 99%

“…The synthesized DNA fragment containing the purN translational initiation site was then transferred to the plasmid pJSl 17 via the PpuMl and BamHl sites to form plasmid pJS193. The former contains a 187-bp sequence (nucleotides 732-919) (Smith & Daum, 1986, 1987 that is part of the purM /purN operon. The remainder of the purN structural gene was added as a 747-bp Sspl DNA fragment derived from pJS18.…”

Section: Construction Of Air Synthase and Gar Tfase Expressionmentioning

confidence: 99%

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Subcloning, characterization, and affinity labeling of Escherichia coli glycinamide ribonucleotide transformylase

Inglese¹,

Dl²,

Shiau³

et al. 1990

Biochemistry

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Glycinamide ribonucleotide transformylase (GAR TFase; EC 2.1.2.2) has been purified 70-fold to apparent homogeneity from Escherichia coli harboring an expression vector encoding the purN gene product, GAR TFase. The protein is a monomer of Mr 23,241 and catalyzes a single reaction. Steady-state kinetic parameters for the enzyme have been obtained. The structural requirements for cofactor utilization have been investigated and found to parallel those of the multifunctional avian enzyme. The enzyme was inactivated with the affinity label N10-(bromoacetyl)-5,8-dideazafolate in a stoichiometric and active-site-specific manner. The ionization state of the cofactor analogue in the enzyme-cofactor complex appears to require the dissociation of the proton at N3 of the pyrimidine within the complex.

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Section: Construction Of Air Synthase and Gar Tfase Expressionmentioning

confidence: 99%

Section: Construction Of Air Synthase and Gar Tfase Expressionmentioning

confidence: 99%

Section: Construction Of Air Synthase and Gar Tfase Expressionmentioning

confidence: 99%

See 1 more Smart Citation

Subcloning, characterization, and affinity labeling of Escherichia coli glycinamide ribonucleotide transformylase

Inglese¹,

Dl²,

Shiau³

et al. 1990

Biochemistry

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show abstract

“…Among the sets of downregulated genes, 24 genes were downregulated under all the 5 conditions, 14 of which are involved in purine or pyrimidine biosynthesis. Interestingly, nine of the ten genes involved in the purine biosynthetic pathway (all but purF), were directly repressed by the regulator PurR [32][33][34][35][36][37][38][39][40].…”

mentioning

confidence: 99%

Gene Networks and Pathways Involved in Escherichia coli Response to Multiple Stressors

et al. 2022

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Stress response helps microorganisms survive extreme environmental conditions and host immunity, making them more virulent or drug resistant. Although both reductionist approaches investigating specific genes and systems approaches analyzing individual stress conditions are being used, less is known about gene networks involved in multiple stress responses. Here, using a systems biology approach, we mined hundreds of transcriptomic data sets for key genes and pathways involved in the tolerance of the model microorganism Escherichia coli to multiple stressors. Specifically, we investigated the E. coli K-12 MG1655 transcriptome under five stresses: heat, cold, oxidative stress, nitrosative stress, and antibiotic treatment. Overlaps of transcriptional changes between studies of each stress factor and between different stressors were determined: energy-requiring metabolic pathways, transport, and motility are typically downregulated to conserve energy, while genes related to survival, bona fide stress response, biofilm formation, and DNA repair are mainly upregulated. The transcription of 15 genes with uncharacterized functions is higher in response to multiple stressors, which suggests they may play pivotal roles in stress response. In conclusion, using rank normalization of transcriptomic data, we identified a set of E. coli stress response genes and pathways, which could be potential targets to overcome antibiotic tolerance or multidrug resistance.

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The production of bacterial cellulose in Gluconacetobacter xylinus regulated by luxR overexpression of quorum sensing system

Zhang

Liu

et al. 2021

Appl Microbiol Biotechnol

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Nucleotide sequence of the purM gene encoding 5'-phosphoribosyl-5-aminoimidazole synthetase of Escherichia coli K12.

Cited by 50 publications

References 35 publications

Subcloning, characterization, and affinity labeling of Escherichia coli glycinamide ribonucleotide transformylase

Subcloning, characterization, and affinity labeling of Escherichia coli glycinamide ribonucleotide transformylase

Gene Networks and Pathways Involved in Escherichia coli Response to Multiple Stressors

The production of bacterial cellulose in Gluconacetobacter xylinus regulated by luxR overexpression of quorum sensing system

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