Nucleotide Sequence of the Tobacco Mosaic Virus (Tomato Strain) Genome and Comparison with the Common Strain Genome1

Abstract

The sequence of about 4,500 nucleotides of the internal part of tobacco mosaic virus (TMV)-tomato strain (L) RNA has been newly determined using cloned cDNAs. Together with the previously determined partial sequences at both ends, the entire sequence of the 6,384 nucleotide genome has been completed. The 130K (1,115 amino acids), 180K (1,615 amino acids), 30K (263 amino acids) and coat protein (158 amino acids) cistrons are located at residues 72-3442, 72-4922, 4906-5700, and 5703-6182 on the genome, respectively. Sequence polymorphism was not observed except for heterogeneity in the length of the A cluster near the 3' end. The homology of the nucleotide sequences of TMV-L and TMV-vulgare, a common strain, is about 80% on average. Remarkable differences between them were found in a part of the N-terminal portion of the 130K/180K protein and the C-terminal portion of the 30K protein. A new method for cDNA cloning was developed by which the cDNA of the 5'-terminus of viral RNA can be cloned efficiently.

The nucleotide sequence of citrus leaf rugose ilarvirus RNA-2

“…The 3' proximal 190 nt of CiLRV RNA-2 were shared with RNA-I and RNA-3 of CiLRV with only slight mismatching (four mismatches with RNA-3 and eight mismatches with RNA-1-unpublished data). This 190 nt region could be folded to form a secondary structure similar to those proposed for A1MV and TSV CiLRV • 64 51 47 48 47 33 32 35 34 34 A1MV 64 • 50 49 52 50 34 35 36 34 32 BMV 51 50 • 85 64 66 38 38 32 35 30 CCMV 47 49 85 • 66 68 41 42 31 38 27 CMV F 48 52 64 66 • 92 38 37 33 34 29 CMVQ 47 50 66 68 92 • 38 37 32 33 29 TMV v 33 34 38 41 38 38 • 89 40 40 35 TMV o 32 35 38 42 37 37 89 • 40 40 36 TRV 35 36 32 31 33 32 40 (1984); CCMV, cowpea chlorotic mottle virus RNA-2, Allison et al (1989); CMVF, cucumber mosaic virus strain Fny RNA-2, Rizzo & Palukaitis (1988); CMVQ, CMV strain Q RNA-2, Rezaian et al (1984); TMVv, tobacco mosaic virus strain vulgare, Goelet et al (1982); TMVo, TMV strain tomato, Ohno et al (1984); TRV, tobacco rattle virus RNA-1, Hamilton et al (1987); PEBV, pea early brown...…”

Nucleotide Sequence of the Genomic RNA of Pepper Mild Mottle Virus, a Resistance-breaking Tobamovirus in Pepper

“…The genome of PMMV-S is 6357 nt long. It shares an overall sequence identity of 69.4% with the RNA of ToMV (Ohno et al, 1984), 68.5% with that of TMV (Goelet et al, 1982) and 64% with TMGMV (Solis & Garcia-Arenal, 1990), the other members of the tobamovirus group whose entire RNA sequences are already known. As shown in Table 1, also, PMMV-S shares a higher degree of amino acid sequence identity with ToMV than with TMV and TMGMV.…”

“…The first one, in the amino part of the protein, has homology with the nsP1 protein of alphaviruses and with the amino part of other proteins implicated in the Ohno et al (1984), Goelet et at. (1982), Meshi et at.…”

“…The complete nucleotide sequence of three other tobamoviruses, tobacco mosaic virus (TMV) (Goelet et al, 1982), tomato mosaic virus (ToMV) (Ohno et al, 1984) and tobacco mild green mottle virus (TMGMV) (Solis & Garcia-Arenal, 1990) have already been reported. The tobamoviral RNA encodes four different proteins: 126K, 183K, 30K and 17.5K, in that order.…”

In vitromutagenesis of the putative replicase genes of tobacco mosaic virus

“…The complete nucleotide sequences of the genomic RNAs of common strain vulgare (2) and tomato strain L (3,4) have been determined and it has been proved that the genomic RNA encodes three non-structural proteins (130K, 180K, 30K proteins) and the coat protein. The 130K and 180K proteins have a common N-terminus (5).…”